Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31274 |
Resumo: | Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. BlaSHV, blaTEM and blaCTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment. |
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Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil Beta-lactamases de espectro estendido em Enterobacteriaceae isoladas de Hospital Público no Brasil ESBLEnterobacteriaceaeKlebsiella pneumoniaePulsed Field Gel ElectrophoresisAntimicrobial resistance Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. BlaSHV, blaTEM and blaCTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment. Beta-lactamases de espectro estendido (ESBL) em enterobactérias são reconhecidas mundialmente como um grande problema hospitalar. Neste estudo, 127 Enterobacteriaceae produtoras de ESBL isoladas por um ano, de pacientes internados e ambulatoriais de um hospital público de ensino em São Paulo, Brasil, foram submetidas à análise pela PCR com iniciadores específicos para os genes blaSHV, blaTEM e blaCTX-M. Dos 127 isolados, 96 (75,6%) K. pneumoniae, 12 (9,3%) E. coli, 8 (6,2%) M. morganii, 3 (2,3%) Proteus mirabilis, 2 (1,6%) Klebsiella oxytoca, 2 (1,6%) Providencia rettgeri, 2 (1,6%) Providencia stuartti, 1 (0,8%) Enterobacter aerogenes e 1 (0,8%) Enterobacter cloacae foram identificados como produtores de ESBL. BlaSHV, blaTEM e blaCTX-M foram detectados em 63%, 17,3% e 33,9% das cepas, respectivamente. A genotipagem de K. pneumoniae por eletroforese em campo pulsado revelou quatro padrões moleculares principais e 29 perfis não relacionados. Os resultados da PCR demonstraram alta variedade de grupos de ESBL entre as cepas, em nove espécies diferentes. Os resultados sugerem a disseminação de genes de resistência entre cepas geneticamente diferentes de K. pneumoniae produtoras de ESBL em algumas unidades do hospital, e também que algumas cepas fortemente relacionadas foram identificadas em unidades hospitalares diferentes, sugerindo disseminação clonal no ambiente da instituição. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31274Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 No. 4 (2009); 203-209 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 Núm. 4 (2009); 203-209 Revista do Instituto de Medicina Tropical de São Paulo; v. 51 n. 4 (2009); 203-209 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31274/33158Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessDropa, MilenaBalsalobre, Livia C.Lincopan, NiltonMamizuka, Elsa M.Murakami, ThaysCassettari, Valéria C.Franco, FábioGuida, Stella M.Balabakis, Angelica J.Passadore, Lilian F.Santos, Silvia R.Matté, Glavur R.Matté, Maria H.2012-07-07T19:23:40Zoai:revistas.usp.br:article/31274Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:57.291380Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil Beta-lactamases de espectro estendido em Enterobacteriaceae isoladas de Hospital Público no Brasil |
title |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil |
spellingShingle |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil Dropa, Milena ESBL Enterobacteriaceae Klebsiella pneumoniae Pulsed Field Gel Electrophoresis Antimicrobial resistance |
title_short |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil |
title_full |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil |
title_fullStr |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil |
title_full_unstemmed |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil |
title_sort |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil |
author |
Dropa, Milena |
author_facet |
Dropa, Milena Balsalobre, Livia C. Lincopan, Nilton Mamizuka, Elsa M. Murakami, Thays Cassettari, Valéria C. Franco, Fábio Guida, Stella M. Balabakis, Angelica J. Passadore, Lilian F. Santos, Silvia R. Matté, Glavur R. Matté, Maria H. |
author_role |
author |
author2 |
Balsalobre, Livia C. Lincopan, Nilton Mamizuka, Elsa M. Murakami, Thays Cassettari, Valéria C. Franco, Fábio Guida, Stella M. Balabakis, Angelica J. Passadore, Lilian F. Santos, Silvia R. Matté, Glavur R. Matté, Maria H. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dropa, Milena Balsalobre, Livia C. Lincopan, Nilton Mamizuka, Elsa M. Murakami, Thays Cassettari, Valéria C. Franco, Fábio Guida, Stella M. Balabakis, Angelica J. Passadore, Lilian F. Santos, Silvia R. Matté, Glavur R. Matté, Maria H. |
dc.subject.por.fl_str_mv |
ESBL Enterobacteriaceae Klebsiella pneumoniae Pulsed Field Gel Electrophoresis Antimicrobial resistance |
topic |
ESBL Enterobacteriaceae Klebsiella pneumoniae Pulsed Field Gel Electrophoresis Antimicrobial resistance |
description |
Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. BlaSHV, blaTEM and blaCTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31274 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31274 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31274/33158 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 No. 4 (2009); 203-209 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 Núm. 4 (2009); 203-209 Revista do Instituto de Medicina Tropical de São Paulo; v. 51 n. 4 (2009); 203-209 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
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Instituto de Medicina Tropical (IMT) |
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IMT |
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IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
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Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
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