Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28619 |
Resumo: | Performance indexes of the peroxidase antibody test were compared to that of the fluorescent antibody test. The peroxidase antibody test had a statistically higher sensitivity and negative predictive value and a higher efficiency than the fluorescent antibody test but its specificity and positive predictive value were within the 95% confidence limits for the values found for the fluorescent antibody test. Such differences did not change when Chagas' disease and visceral leishmaniasis sera were included in index calculations. Statistical analysis showed that the two tests have a substantial degree of agreement but the immunofluorescent test had a specificity index and a positive predictive value equal to 100.0% when Chagas' disease and visceral leishmaniasis sera were not included in the calculations of the performance index; in this instance, a positive test result equals a disclosure of the disease attribute due to the inexistence of false positive results. The enzyme/ protein ratio of the peroxidase conjugate, resulting in heavy or light-labeled conjugates may pose technical problems to its use in serology tests. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência Teste de imunoperoxidase para a sorologia da leishmaniose mueocutânea Mucocutaneous leishmaniasis serologyPerformance indexPeroxidase antibody testFluorescent antibody test Performance indexes of the peroxidase antibody test were compared to that of the fluorescent antibody test. The peroxidase antibody test had a statistically higher sensitivity and negative predictive value and a higher efficiency than the fluorescent antibody test but its specificity and positive predictive value were within the 95% confidence limits for the values found for the fluorescent antibody test. Such differences did not change when Chagas' disease and visceral leishmaniasis sera were included in index calculations. Statistical analysis showed that the two tests have a substantial degree of agreement but the immunofluorescent test had a specificity index and a positive predictive value equal to 100.0% when Chagas' disease and visceral leishmaniasis sera were not included in the calculations of the performance index; in this instance, a positive test result equals a disclosure of the disease attribute due to the inexistence of false positive results. The enzyme/ protein ratio of the peroxidase conjugate, resulting in heavy or light-labeled conjugates may pose technical problems to its use in serology tests. Os parâmetros sorológicos do teste de imunoperoxidase foram comparados aos do teste de imunofluorescência. O teste de imunoperoxidase mostrou ter sensibilidade e valor de predição negativo estatisticamente mais alto que aqueles do teste de imunofluorescência porém, os limites de confiança 95% da especificidade e do valor de predição positivo estavam contidos naqueles encontrados para o teste de imunofluorescência. Tais diferenças se mantiveram quando os cálculos dos índices foram feitos com e sem a inclusão de soros de doença de Chagas ou leishmaniose visceral. A análise estatística mostrou que os dois testes tinham um grau substancial de concordância mas o teste de imunofluorescência tinha um índice de especificidade e o vaior de predição positivo igual a 100,0% quando os soros de Chagas e leishmaniose visceral foram excluídos. Neste caso. o teste positivo se torna o teste diagnóstico da doença em face da não existência de falso-positivos. O conjugado de peroxidase poderá se constituir em fonte de problemas técnicos na sorologia se a relação enzima proteína se afastar das quantidades ótimas de marcação. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1988-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28619Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 6 (1988); 411-414 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 6 (1988); 411-414 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 6 (1988); 411-414 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28619/30472Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCeleste, Beatriz J.Guimarães, M. Carolina S.Corrales L., Edelma Maria2012-07-02T01:07:12Zoai:revistas.usp.br:article/28619Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:21.757610Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência Teste de imunoperoxidase para a sorologia da leishmaniose mueocutânea |
title |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência |
spellingShingle |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência Celeste, Beatriz J. Mucocutaneous leishmaniasis serology Performance index Peroxidase antibody test Fluorescent antibody test |
title_short |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência |
title_full |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência |
title_fullStr |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência |
title_full_unstemmed |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência |
title_sort |
Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência |
author |
Celeste, Beatriz J. |
author_facet |
Celeste, Beatriz J. Guimarães, M. Carolina S. Corrales L., Edelma Maria |
author_role |
author |
author2 |
Guimarães, M. Carolina S. Corrales L., Edelma Maria |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Celeste, Beatriz J. Guimarães, M. Carolina S. Corrales L., Edelma Maria |
dc.subject.por.fl_str_mv |
Mucocutaneous leishmaniasis serology Performance index Peroxidase antibody test Fluorescent antibody test |
topic |
Mucocutaneous leishmaniasis serology Performance index Peroxidase antibody test Fluorescent antibody test |
description |
Performance indexes of the peroxidase antibody test were compared to that of the fluorescent antibody test. The peroxidase antibody test had a statistically higher sensitivity and negative predictive value and a higher efficiency than the fluorescent antibody test but its specificity and positive predictive value were within the 95% confidence limits for the values found for the fluorescent antibody test. Such differences did not change when Chagas' disease and visceral leishmaniasis sera were included in index calculations. Statistical analysis showed that the two tests have a substantial degree of agreement but the immunofluorescent test had a specificity index and a positive predictive value equal to 100.0% when Chagas' disease and visceral leishmaniasis sera were not included in the calculations of the performance index; in this instance, a positive test result equals a disclosure of the disease attribute due to the inexistence of false positive results. The enzyme/ protein ratio of the peroxidase conjugate, resulting in heavy or light-labeled conjugates may pose technical problems to its use in serology tests. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28619 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28619 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28619/30472 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 6 (1988); 411-414 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 6 (1988); 411-414 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 6 (1988); 411-414 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951637827452928 |