Co-infection between influenza virus and flagellated bacteria

Detalhes bibliográficos
Autor(a) principal: Mancini, Dalva Assunção Portari
Data de Publicação: 2005
Outros Autores: Mendonça, Rita Maria Zucatelli, Dias, Andrea Luppi Fernandes, Mendonça, Ronaldo Zucatelli, Pinto, José Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30938
Resumo: Trypsin is required in the hemagglutinin (HA) cleavage to in vitro influenza viruses activation. This HA cleavage is necessary for virus cell entry by receptor-mediated endocytosis. Bacteria in the respiratory tract are potential sources of proteases that could contribute to the cleavage of influenza virus in vivo. From 47 samples collected from horses, pigs, and from humans, influenza presence was confirmed in 13 and these samples demonstrated co-infection of influenza with flagellated bacteria, Stenotrophomonas maltophilia from the beginning of the experiments. Despite treatment with antibiotics, the bacteria remained resistant in several of the co-infected samples (48.39%). These bacteria, considered opportunistic invaders from environmental sources, are associated with viral infections in upper respiratory tract of hosts. The protease (elastase), secreted by Stenotrophomonas maltophilia plays a role in the potentiation of influenza virus infection. Proteolytic activity was detected by casein agar test. Positive samples from animals and humans had either a potentiated influenza infectivity or cytopathic effect (CPE) in MDCK and NCI H292 cells, Stenotrophomonas maltophilia were always present. Virus and bacteria were observed ultrastructurally. These in vitro findings show that microbial proteases could contribute to respiratory complications by host protease activity increasing inflammation or destroying endogenous cell protease inhibitors.
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spelling Co-infection between influenza virus and flagellated bacteria Co-infecção entre vírus influenza e bactéria flagelada Stenotrophomonas maltophiliaInfluenza virusCo-infections in vitro Trypsin is required in the hemagglutinin (HA) cleavage to in vitro influenza viruses activation. This HA cleavage is necessary for virus cell entry by receptor-mediated endocytosis. Bacteria in the respiratory tract are potential sources of proteases that could contribute to the cleavage of influenza virus in vivo. From 47 samples collected from horses, pigs, and from humans, influenza presence was confirmed in 13 and these samples demonstrated co-infection of influenza with flagellated bacteria, Stenotrophomonas maltophilia from the beginning of the experiments. Despite treatment with antibiotics, the bacteria remained resistant in several of the co-infected samples (48.39%). These bacteria, considered opportunistic invaders from environmental sources, are associated with viral infections in upper respiratory tract of hosts. The protease (elastase), secreted by Stenotrophomonas maltophilia plays a role in the potentiation of influenza virus infection. Proteolytic activity was detected by casein agar test. Positive samples from animals and humans had either a potentiated influenza infectivity or cytopathic effect (CPE) in MDCK and NCI H292 cells, Stenotrophomonas maltophilia were always present. Virus and bacteria were observed ultrastructurally. These in vitro findings show that microbial proteases could contribute to respiratory complications by host protease activity increasing inflammation or destroying endogenous cell protease inhibitors. Tripsina é necessária na ativação da clivagem do vírus influenza A in vitro. Esta clivagem é importante para entrada do vírus na célula por endocitose mediada pelo receptor celular. Bactérias presentes no trato respiratório são fontes de proteases que podem contribuir na replicação do vírus influenza in vivo. Entre 47 amostras coletadas de cavalos, suínos e humanos, a influenza foi isolada e confirmada em 13 que estavam co-infectadas com bactéria flagelada: Stenotrophomonas maltophilia desde o início destes experimentos. Apesar do tratamento das amostras com antibióticos, as bactérias resistiram em diversas delas (48.39%). A protease (elastase), secretada pela Stenotrophomonas maltophilia, desenvolveu papel decisivo na potencialização da infecção pelo vírus influenza. Essa atividade proteolítica foi detectada pelo teste de ágar-caseína. Amostras positivas para o vírus influenza isolado em animais, bem como em humanos tiveram potencialização da infectividade (ECP) em células MDCK e NCI-H292, sempre que a Stenotrophomonas maltophilia esteve presente. Os referidos microorganismos, bactéria e vírus foram observados ultra-estruturalmente. Esses achados in vitro demonstram como complicações respiratórias podem ocorrer in vivo, através da contribuição de protease microbiana, provocando aumento da inflamação ou destruição dos inibidores celulares de proteases endógenas, nos hospedeiros susceptíveis à influenza. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30938Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 No. 5 (2005); 275-280 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 Núm. 5 (2005); 275-280 Revista do Instituto de Medicina Tropical de São Paulo; v. 47 n. 5 (2005); 275-280 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30938/32822Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMancini, Dalva Assunção PortariMendonça, Rita Maria ZucatelliDias, Andrea Luppi FernandesMendonça, Ronaldo ZucatelliPinto, José Ricardo2012-07-07T18:38:22Zoai:revistas.usp.br:article/30938Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:39.707406Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Co-infection between influenza virus and flagellated bacteria
Co-infecção entre vírus influenza e bactéria flagelada
title Co-infection between influenza virus and flagellated bacteria
spellingShingle Co-infection between influenza virus and flagellated bacteria
Mancini, Dalva Assunção Portari
Stenotrophomonas maltophilia
Influenza virus
Co-infections in vitro
title_short Co-infection between influenza virus and flagellated bacteria
title_full Co-infection between influenza virus and flagellated bacteria
title_fullStr Co-infection between influenza virus and flagellated bacteria
title_full_unstemmed Co-infection between influenza virus and flagellated bacteria
title_sort Co-infection between influenza virus and flagellated bacteria
author Mancini, Dalva Assunção Portari
author_facet Mancini, Dalva Assunção Portari
Mendonça, Rita Maria Zucatelli
Dias, Andrea Luppi Fernandes
Mendonça, Ronaldo Zucatelli
Pinto, José Ricardo
author_role author
author2 Mendonça, Rita Maria Zucatelli
Dias, Andrea Luppi Fernandes
Mendonça, Ronaldo Zucatelli
Pinto, José Ricardo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mancini, Dalva Assunção Portari
Mendonça, Rita Maria Zucatelli
Dias, Andrea Luppi Fernandes
Mendonça, Ronaldo Zucatelli
Pinto, José Ricardo
dc.subject.por.fl_str_mv Stenotrophomonas maltophilia
Influenza virus
Co-infections in vitro
topic Stenotrophomonas maltophilia
Influenza virus
Co-infections in vitro
description Trypsin is required in the hemagglutinin (HA) cleavage to in vitro influenza viruses activation. This HA cleavage is necessary for virus cell entry by receptor-mediated endocytosis. Bacteria in the respiratory tract are potential sources of proteases that could contribute to the cleavage of influenza virus in vivo. From 47 samples collected from horses, pigs, and from humans, influenza presence was confirmed in 13 and these samples demonstrated co-infection of influenza with flagellated bacteria, Stenotrophomonas maltophilia from the beginning of the experiments. Despite treatment with antibiotics, the bacteria remained resistant in several of the co-infected samples (48.39%). These bacteria, considered opportunistic invaders from environmental sources, are associated with viral infections in upper respiratory tract of hosts. The protease (elastase), secreted by Stenotrophomonas maltophilia plays a role in the potentiation of influenza virus infection. Proteolytic activity was detected by casein agar test. Positive samples from animals and humans had either a potentiated influenza infectivity or cytopathic effect (CPE) in MDCK and NCI H292 cells, Stenotrophomonas maltophilia were always present. Virus and bacteria were observed ultrastructurally. These in vitro findings show that microbial proteases could contribute to respiratory complications by host protease activity increasing inflammation or destroying endogenous cell protease inhibitors.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30938
url https://www.revistas.usp.br/rimtsp/article/view/30938
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30938/32822
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 No. 5 (2005); 275-280
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 Núm. 5 (2005); 275-280
Revista do Instituto de Medicina Tropical de São Paulo; v. 47 n. 5 (2005); 275-280
1678-9946
0036-4665
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
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