Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers

Detalhes bibliográficos
Autor(a) principal: Loría-Cervera, Elsy Nalleli
Data de Publicação: 2024
Outros Autores: Sosa-Bibiano, Erika Ivett, López-Ávila, Karina Beatriz, Oca-Aguilar, Ana Celia Montes de, Moreno-Nava, Marisol Sarahí, Torres-Castro, Jimmy Raymundo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/222565
Resumo: Asymptomatic infection (the absence or inapparent signs and symptoms) has been observed in many endemic areas of leishmaniasis, however, little is known about the parasitological and immunological factors associated with this type of infection. This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, and no correlation was found between the presence of viable parasites and the expression of IFN-γ. This study demonstrates the persistence of Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers from Mexico.
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spelling Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriersLeishmaniasisCutaneous leishmaniasisAsymptomatic infectionImmune responseIFN-γ expressionAsymptomatic infection (the absence or inapparent signs and symptoms) has been observed in many endemic areas of leishmaniasis, however, little is known about the parasitological and immunological factors associated with this type of infection. This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, and no correlation was found between the presence of viable parasites and the expression of IFN-γ. This study demonstrates the persistence of Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers from Mexico.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2024-02-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/22256510.1590/S1678-9946202466013 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e13Revista do Instituto de Medicina Tropical de São Paulo; v. 66 (2024); e13Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e131678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/222565/203075Copyright (c) 2024 Elsy Nalleli Loría-Cervera, Erika Ivett Sosa-Bibiano, Karina Beatriz López-Ávila, Ana Celia Montes de Oca-Aguilar, Marisol Sarahí Moreno-Nava, Jimmy Raymundo Torres-Castrohttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLoría-Cervera, Elsy Nalleli Sosa-Bibiano, Erika Ivett López-Ávila, Karina Beatriz Oca-Aguilar, Ana Celia Montes de Moreno-Nava, Marisol Sarahí Torres-Castro, Jimmy Raymundo 2024-03-20T14:09:30Zoai:revistas.usp.br:article/222565Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2024-03-20T14:09:30Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false
dc.title.none.fl_str_mv Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
title Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
spellingShingle Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
Loría-Cervera, Elsy Nalleli
Leishmaniasis
Cutaneous leishmaniasis
Asymptomatic infection
Immune response
IFN-γ expression
title_short Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
title_full Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
title_fullStr Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
title_full_unstemmed Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
title_sort Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
author Loría-Cervera, Elsy Nalleli
author_facet Loría-Cervera, Elsy Nalleli
Sosa-Bibiano, Erika Ivett
López-Ávila, Karina Beatriz
Oca-Aguilar, Ana Celia Montes de
Moreno-Nava, Marisol Sarahí
Torres-Castro, Jimmy Raymundo
author_role author
author2 Sosa-Bibiano, Erika Ivett
López-Ávila, Karina Beatriz
Oca-Aguilar, Ana Celia Montes de
Moreno-Nava, Marisol Sarahí
Torres-Castro, Jimmy Raymundo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Loría-Cervera, Elsy Nalleli
Sosa-Bibiano, Erika Ivett
López-Ávila, Karina Beatriz
Oca-Aguilar, Ana Celia Montes de
Moreno-Nava, Marisol Sarahí
Torres-Castro, Jimmy Raymundo
dc.subject.por.fl_str_mv Leishmaniasis
Cutaneous leishmaniasis
Asymptomatic infection
Immune response
IFN-γ expression
topic Leishmaniasis
Cutaneous leishmaniasis
Asymptomatic infection
Immune response
IFN-γ expression
description Asymptomatic infection (the absence or inapparent signs and symptoms) has been observed in many endemic areas of leishmaniasis, however, little is known about the parasitological and immunological factors associated with this type of infection. This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, and no correlation was found between the presence of viable parasites and the expression of IFN-γ. This study demonstrates the persistence of Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers from Mexico.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/222565
10.1590/S1678-9946202466013
url https://www.revistas.usp.br/rimtsp/article/view/222565
identifier_str_mv 10.1590/S1678-9946202466013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/222565/203075
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e13
Revista do Instituto de Medicina Tropical de São Paulo; v. 66 (2024); e13
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e13
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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