Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/222565 |
Resumo: | Asymptomatic infection (the absence or inapparent signs and symptoms) has been observed in many endemic areas of leishmaniasis, however, little is known about the parasitological and immunological factors associated with this type of infection. This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, and no correlation was found between the presence of viable parasites and the expression of IFN-γ. This study demonstrates the persistence of Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers from Mexico. |
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Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriersLeishmaniasisCutaneous leishmaniasisAsymptomatic infectionImmune responseIFN-γ expressionAsymptomatic infection (the absence or inapparent signs and symptoms) has been observed in many endemic areas of leishmaniasis, however, little is known about the parasitological and immunological factors associated with this type of infection. This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, and no correlation was found between the presence of viable parasites and the expression of IFN-γ. This study demonstrates the persistence of Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers from Mexico.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2024-02-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/22256510.1590/S1678-9946202466013 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e13Revista do Instituto de Medicina Tropical de São Paulo; v. 66 (2024); e13Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e131678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/222565/203075Copyright (c) 2024 Elsy Nalleli Loría-Cervera, Erika Ivett Sosa-Bibiano, Karina Beatriz López-Ávila, Ana Celia Montes de Oca-Aguilar, Marisol Sarahí Moreno-Nava, Jimmy Raymundo Torres-Castrohttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLoría-Cervera, Elsy Nalleli Sosa-Bibiano, Erika Ivett López-Ávila, Karina Beatriz Oca-Aguilar, Ana Celia Montes de Moreno-Nava, Marisol Sarahí Torres-Castro, Jimmy Raymundo 2024-03-20T14:09:30Zoai:revistas.usp.br:article/222565Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2024-03-20T14:09:30Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false |
dc.title.none.fl_str_mv |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
title |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
spellingShingle |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers Loría-Cervera, Elsy Nalleli Leishmaniasis Cutaneous leishmaniasis Asymptomatic infection Immune response IFN-γ expression |
title_short |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
title_full |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
title_fullStr |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
title_full_unstemmed |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
title_sort |
Viable Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers |
author |
Loría-Cervera, Elsy Nalleli |
author_facet |
Loría-Cervera, Elsy Nalleli Sosa-Bibiano, Erika Ivett López-Ávila, Karina Beatriz Oca-Aguilar, Ana Celia Montes de Moreno-Nava, Marisol Sarahí Torres-Castro, Jimmy Raymundo |
author_role |
author |
author2 |
Sosa-Bibiano, Erika Ivett López-Ávila, Karina Beatriz Oca-Aguilar, Ana Celia Montes de Moreno-Nava, Marisol Sarahí Torres-Castro, Jimmy Raymundo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Loría-Cervera, Elsy Nalleli Sosa-Bibiano, Erika Ivett López-Ávila, Karina Beatriz Oca-Aguilar, Ana Celia Montes de Moreno-Nava, Marisol Sarahí Torres-Castro, Jimmy Raymundo |
dc.subject.por.fl_str_mv |
Leishmaniasis Cutaneous leishmaniasis Asymptomatic infection Immune response IFN-γ expression |
topic |
Leishmaniasis Cutaneous leishmaniasis Asymptomatic infection Immune response IFN-γ expression |
description |
Asymptomatic infection (the absence or inapparent signs and symptoms) has been observed in many endemic areas of leishmaniasis, however, little is known about the parasitological and immunological factors associated with this type of infection. This study aimed to identify the in vitro expression of IFN-γ in asymptomatic carriers of viable Leishmania parasites. Asymptomatic infection was identified using the Montenegro skin test in an at-risk population from Yucatan, Mexico. Parasite viability was evinced in the blood by 7SL RNA transcripts amplification. The expression of mRNA IFN-γ was analyzed in peripheral blood mononuclear cells stimulated with soluble Leishmania antigen, using RT-qPCR. Parasite viability was observed in 33.3 % (5/15) of asymptomatic subjects. No differences were found in the expression of IFN-γ between asymptomatic and healthy subjects, and no correlation was found between the presence of viable parasites and the expression of IFN-γ. This study demonstrates the persistence of Leishmania parasites in the absence of an in vitro IFN-γ response in asymptomatic carriers from Mexico. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/222565 10.1590/S1678-9946202466013 |
url |
https://www.revistas.usp.br/rimtsp/article/view/222565 |
identifier_str_mv |
10.1590/S1678-9946202466013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/222565/203075 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e13 Revista do Instituto de Medicina Tropical de São Paulo; v. 66 (2024); e13 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e13 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951636656193536 |