Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/144543 |
Resumo: | A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage. |
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Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virusZika virusRubella virusIsolationGenotype A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/144543Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e15Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e15Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e151678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/144543/138868Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessOliveira, Maria Isabel deNamiyama, Gislene MitsueCabral, Gabriela BastosFerreira, João LeandroTaniwaki, NoemiAfonso, Ana Maria SardinhaLima, Isabella RilloBrigido, Luís Fernando Macedo de2018-07-12T16:41:33Zoai:revistas.usp.br:article/144543Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:43.927274Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
title |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
spellingShingle |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus Oliveira, Maria Isabel de Zika virus Rubella virus Isolation Genotype |
title_short |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
title_full |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
title_fullStr |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
title_full_unstemmed |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
title_sort |
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus |
author |
Oliveira, Maria Isabel de |
author_facet |
Oliveira, Maria Isabel de Namiyama, Gislene Mitsue Cabral, Gabriela Bastos Ferreira, João Leandro Taniwaki, Noemi Afonso, Ana Maria Sardinha Lima, Isabella Rillo Brigido, Luís Fernando Macedo de |
author_role |
author |
author2 |
Namiyama, Gislene Mitsue Cabral, Gabriela Bastos Ferreira, João Leandro Taniwaki, Noemi Afonso, Ana Maria Sardinha Lima, Isabella Rillo Brigido, Luís Fernando Macedo de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Maria Isabel de Namiyama, Gislene Mitsue Cabral, Gabriela Bastos Ferreira, João Leandro Taniwaki, Noemi Afonso, Ana Maria Sardinha Lima, Isabella Rillo Brigido, Luís Fernando Macedo de |
dc.subject.por.fl_str_mv |
Zika virus Rubella virus Isolation Genotype |
topic |
Zika virus Rubella virus Isolation Genotype |
description |
A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/144543 |
url |
https://www.revistas.usp.br/rimtsp/article/view/144543 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/144543/138868 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e15 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e15 Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e15 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951652109058048 |