A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)

Detalhes bibliográficos
Autor(a) principal: ROCHA, Sérgio M.
Data de Publicação: 2002
Outros Autores: SUZUKI, Lisandra A., SILVA, Andréa D.T. da, ARRUDA, Gisele C., ROSSI, Cláudio L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30598
Resumo: Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases. In this article, we present the preliminary results obtained with a latex agglutination test (LAT) for diagnosing neurocysticercosis by detection of antibodies in CSF. A total of 43 CSF samples were assayed by the LAT: 19 CSF samples from patients with neurocysticercosis and 24 CSF samples from patients with other neurologic disorders (neurosyphilis, n = 8; neurotoxoplasmosis, n = 3; viral meningitis, n = 4, chronic headache, n = 9). The LAT exhibited 89.5% sensitivity and 75% specificity. The use of LAT seems to be an additional approach for the screening of neurocysticercosis with advantage of simplicity and rapidity. Further studies could be performed using purified antigens and serum samples.
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spelling A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF) Teste rápido de aglutinação utilizando partículas de látex para a detecção de anticorpos anti-cisticercos em amostras de líquido cefalorraquidiano (LCR) Taenia solium cysticercusLatex agglutination testAntibody detection Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases. In this article, we present the preliminary results obtained with a latex agglutination test (LAT) for diagnosing neurocysticercosis by detection of antibodies in CSF. A total of 43 CSF samples were assayed by the LAT: 19 CSF samples from patients with neurocysticercosis and 24 CSF samples from patients with other neurologic disorders (neurosyphilis, n = 8; neurotoxoplasmosis, n = 3; viral meningitis, n = 4, chronic headache, n = 9). The LAT exhibited 89.5% sensitivity and 75% specificity. The use of LAT seems to be an additional approach for the screening of neurocysticercosis with advantage of simplicity and rapidity. Further studies could be performed using purified antigens and serum samples. Testes diagnósticos simples e rápidos baseados na aglutinação de partículas de látex têm sido utilizados para a pequisa de antígenos ou anticorpos específicos em muitas doenças. No presente trabalho, é descrito um teste de aglutinação em lâmina para a pesquisa de anticorpos contra cisticercos de Taenia solium, utilizando partículas de látex revestidas com um extrato bruto do parasita. Anticorpos anti-cisticercos foram pesquisados em 19 amostras de LCR de pacientes com neurocisticercose e em 24 amostras de LCR de pacientes com outros problemas neurológicos (neurosífilis, n = 8; neurotoxoplasmose, n = 3; meningite viral, n = 4; cefaléia crônica, n = 9). O teste de aglutinação apresentou sensibilidade e especificidade de 89,5% e 75%, respectivamente. O teste de aglutinação para cisticercose idealizado é simples, rápido e barato. Essas características tornam o teste um meio promissor de expansão e simplificação do imunodiagnósico da neurocisticercose. Estudos futuros poderiam testar a sensibilização das partículas de látex com antígenos de cisticercos purificados e a pesquisa de anticorpos em amostras de soros. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2002-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30598Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 1 (2002); 57-58 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 1 (2002); 57-58 Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 1 (2002); 57-58 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30598/32482Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessROCHA, Sérgio M.SUZUKI, Lisandra A.SILVA, Andréa D.T. daARRUDA, Gisele C.ROSSI, Cláudio L.2012-07-07T17:50:53Zoai:revistas.usp.br:article/30598Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:22.836721Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
Teste rápido de aglutinação utilizando partículas de látex para a detecção de anticorpos anti-cisticercos em amostras de líquido cefalorraquidiano (LCR)
title A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
spellingShingle A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
ROCHA, Sérgio M.
Taenia solium cysticercus
Latex agglutination test
Antibody detection
title_short A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
title_full A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
title_fullStr A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
title_full_unstemmed A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
title_sort A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)
author ROCHA, Sérgio M.
author_facet ROCHA, Sérgio M.
SUZUKI, Lisandra A.
SILVA, Andréa D.T. da
ARRUDA, Gisele C.
ROSSI, Cláudio L.
author_role author
author2 SUZUKI, Lisandra A.
SILVA, Andréa D.T. da
ARRUDA, Gisele C.
ROSSI, Cláudio L.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv ROCHA, Sérgio M.
SUZUKI, Lisandra A.
SILVA, Andréa D.T. da
ARRUDA, Gisele C.
ROSSI, Cláudio L.
dc.subject.por.fl_str_mv Taenia solium cysticercus
Latex agglutination test
Antibody detection
topic Taenia solium cysticercus
Latex agglutination test
Antibody detection
description Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases. In this article, we present the preliminary results obtained with a latex agglutination test (LAT) for diagnosing neurocysticercosis by detection of antibodies in CSF. A total of 43 CSF samples were assayed by the LAT: 19 CSF samples from patients with neurocysticercosis and 24 CSF samples from patients with other neurologic disorders (neurosyphilis, n = 8; neurotoxoplasmosis, n = 3; viral meningitis, n = 4, chronic headache, n = 9). The LAT exhibited 89.5% sensitivity and 75% specificity. The use of LAT seems to be an additional approach for the screening of neurocysticercosis with advantage of simplicity and rapidity. Further studies could be performed using purified antigens and serum samples.
publishDate 2002
dc.date.none.fl_str_mv 2002-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30598
url https://www.revistas.usp.br/rimtsp/article/view/30598
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30598/32482
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 1 (2002); 57-58
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 1 (2002); 57-58
Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 1 (2002); 57-58
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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