Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test

Detalhes bibliográficos
Autor(a) principal: Landgraf, Ilka Maria
Data de Publicação: 1995
Outros Autores: Alkmin, Maria das Graças Adelino, Vieira, Maria de Fátima Paiva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29271
Resumo: Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C.
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spelling Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test Detecção de antígenos bacterianos no líquido cefalorraquidiano através do teste de aglutinação de latex Bacterial meningitisLatex agglutinationBacterial antigen detection in CSF Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C. Oitenta amostras purulentas de líquido cefalorraquidiano (LCR) de pacientes com evidência clínica de meningite foram estudadas empregando-se Kit Directigen de aglutinação de latex (AL) para demonstrar antígeno bacteriano no LCR. Os resultados mostraram que o teste de AL apresentou melhor desempenho diagnóstico do que bacterioscopia através da coloração de Gram, cultura e imunoeletroforese cruzada (IEC) em relação à Neisseria meningitidis grupos B e C, e ao Haemophilus influenzae tipo b, e melhor do que coloração de Gram e cultura quando Streptococcus pneumoniae foi avaliado. A comparação dos resultados com os de cultura mostrou o maior nível de sensibilidade considerando-se N. meningitidis grupo C. Quanto à especificidade, os valores foram satisfatórios para todos os microrganismos testados. O grau de concordância K em relação à IEC exibiu melhores índices K de concordância para N. meningitidis grupos B e C. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29271Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 3 (1995); 257-260 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 3 (1995); 257-260 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 3 (1995); 257-260 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29271/31128Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessLandgraf, Ilka MariaAlkmin, Maria das Graças AdelinoVieira, Maria de Fátima Paiva2012-07-02T01:40:18Zoai:revistas.usp.br:article/29271Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:01.232258Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
Detecção de antígenos bacterianos no líquido cefalorraquidiano através do teste de aglutinação de latex
title Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
spellingShingle Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
Landgraf, Ilka Maria
Bacterial meningitis
Latex agglutination
Bacterial antigen detection in CSF
title_short Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
title_full Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
title_fullStr Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
title_full_unstemmed Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
title_sort Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test
author Landgraf, Ilka Maria
author_facet Landgraf, Ilka Maria
Alkmin, Maria das Graças Adelino
Vieira, Maria de Fátima Paiva
author_role author
author2 Alkmin, Maria das Graças Adelino
Vieira, Maria de Fátima Paiva
author2_role author
author
dc.contributor.author.fl_str_mv Landgraf, Ilka Maria
Alkmin, Maria das Graças Adelino
Vieira, Maria de Fátima Paiva
dc.subject.por.fl_str_mv Bacterial meningitis
Latex agglutination
Bacterial antigen detection in CSF
topic Bacterial meningitis
Latex agglutination
Bacterial antigen detection in CSF
description Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C.
publishDate 1995
dc.date.none.fl_str_mv 1995-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29271
url https://www.revistas.usp.br/rimtsp/article/view/29271
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29271/31128
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 3 (1995); 257-260
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 3 (1995); 257-260
Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 3 (1995); 257-260
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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