Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30663 |
Resumo: | In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively. |
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Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection Caracterização molecular de vírus Dengue tipo 1 e 2 isolados de um paciente com dupla infecção Dengue virusDual infectionRT/PCRE/NS1 gene sequencesPhylogenetic analysis In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively. A cidade de Barretos, com cerca de 105.000 habitantes e situada na região norte do Estado de São Paulo, apresentou, no ano de 2001, importante epidemia de dengue causada por vírus dengue de sorotipos 1 e 2. Nesta epidemia foi detectado um caso de dupla infecção viral em um paciente acometido pela forma clássica da doença. Os vírus foram isolados em cultura de células C6/36 e identificados como vírus dengue 1 e 2 por reações de imunofluorescência e RT-PCR. A análise molecular por seqüenciamento de nucleotídeos da junção dos genes E/NS1 dos vírus em estudo mostrou a presença de regiões bem conservadas quando comparados aos vírus protótipos, observando-se algumas alterações nucleotídicas que em sua maioria originaram mutações silenciosas. Resultados da análise de verossimilhança máxima para a inferência filogenética das seqüências E/NS1 indicaram que os vírus dengue 1 e 2 isolados do paciente pertencem aos genótipos I e III respectivamente. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30663Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 1 (2003); 11-16 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 1 (2003); 11-16 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 1 (2003); 11-16 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30663/32547Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSantos, Cecília Luiza Simões dosBastos, Mariana Aparecida AntunesSallum, Maria Anice MurebRocco, Iray Maria2012-07-07T18:00:05Zoai:revistas.usp.br:article/30663Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:26.118444Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection Caracterização molecular de vírus Dengue tipo 1 e 2 isolados de um paciente com dupla infecção |
title |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection |
spellingShingle |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection Santos, Cecília Luiza Simões dos Dengue virus Dual infection RT/PCR E/NS1 gene sequences Phylogenetic analysis |
title_short |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection |
title_full |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection |
title_fullStr |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection |
title_full_unstemmed |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection |
title_sort |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection |
author |
Santos, Cecília Luiza Simões dos |
author_facet |
Santos, Cecília Luiza Simões dos Bastos, Mariana Aparecida Antunes Sallum, Maria Anice Mureb Rocco, Iray Maria |
author_role |
author |
author2 |
Bastos, Mariana Aparecida Antunes Sallum, Maria Anice Mureb Rocco, Iray Maria |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santos, Cecília Luiza Simões dos Bastos, Mariana Aparecida Antunes Sallum, Maria Anice Mureb Rocco, Iray Maria |
dc.subject.por.fl_str_mv |
Dengue virus Dual infection RT/PCR E/NS1 gene sequences Phylogenetic analysis |
topic |
Dengue virus Dual infection RT/PCR E/NS1 gene sequences Phylogenetic analysis |
description |
In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30663 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30663 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30663/32547 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 1 (2003); 11-16 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 1 (2003); 11-16 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 1 (2003); 11-16 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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