Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys

Detalhes bibliográficos
Autor(a) principal: Ferreira, Antonio Walter
Data de Publicação: 1988
Outros Autores: Sanchez, Maria Carmen Arroyo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28578
Resumo: The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtained when sera were diluted in PBS containing 1% Tween-80 and the slides, containing the antigenic preparation were fixed in cold acetone or stabilized on dried air with silica. The ELISA test was standardized for P. falciparum antibodies and the comparison of the IFA and ELISA showed: a) in P. falciparum prime infected patient the sensitivity was 71% for both tests; b) in P. vivax prime infected patients the sensitivity was 40% for both tests; c) in non prime infected patients with P. falciparum malaria the sensitivity was 100% for both tests; d) in non prime infected patients with P. vivax malaria the sensitivity was 85% for ELISA and 92% for IFA; e) in patients with P. vivax and P. falciparum malaria the sensitivity was 100% for both tests. The specificity was 95% for ELISA and 100% for IFA in non malaria individuals. The results showed that the ELISA test could be an alternative for IFA for IgG antibodies in the serology of malaria.
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spelling Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys Malária humana: padronização e optimização de testes sorológicos para diagnóstico individual e inquéritos soroepidemiológicos MaláriaIFITeste imunoenzimático ELISA The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtained when sera were diluted in PBS containing 1% Tween-80 and the slides, containing the antigenic preparation were fixed in cold acetone or stabilized on dried air with silica. The ELISA test was standardized for P. falciparum antibodies and the comparison of the IFA and ELISA showed: a) in P. falciparum prime infected patient the sensitivity was 71% for both tests; b) in P. vivax prime infected patients the sensitivity was 40% for both tests; c) in non prime infected patients with P. falciparum malaria the sensitivity was 100% for both tests; d) in non prime infected patients with P. vivax malaria the sensitivity was 85% for ELISA and 92% for IFA; e) in patients with P. vivax and P. falciparum malaria the sensitivity was 100% for both tests. The specificity was 95% for ELISA and 100% for IFA in non malaria individuals. The results showed that the ELISA test could be an alternative for IFA for IgG antibodies in the serology of malaria. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1988-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28578Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 3 (1988); 137-146 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 3 (1988); 137-146 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 3 (1988); 137-146 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28578/30431Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFerreira, Antonio WalterSanchez, Maria Carmen Arroyo2012-07-02T01:04:53Zoai:revistas.usp.br:article/28578Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:18.920854Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
Malária humana: padronização e optimização de testes sorológicos para diagnóstico individual e inquéritos soroepidemiológicos
title Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
spellingShingle Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
Ferreira, Antonio Walter
Malária
IFI
Teste imunoenzimático ELISA
title_short Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
title_full Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
title_fullStr Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
title_full_unstemmed Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
title_sort Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
author Ferreira, Antonio Walter
author_facet Ferreira, Antonio Walter
Sanchez, Maria Carmen Arroyo
author_role author
author2 Sanchez, Maria Carmen Arroyo
author2_role author
dc.contributor.author.fl_str_mv Ferreira, Antonio Walter
Sanchez, Maria Carmen Arroyo
dc.subject.por.fl_str_mv Malária
IFI
Teste imunoenzimático ELISA
topic Malária
IFI
Teste imunoenzimático ELISA
description The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtained when sera were diluted in PBS containing 1% Tween-80 and the slides, containing the antigenic preparation were fixed in cold acetone or stabilized on dried air with silica. The ELISA test was standardized for P. falciparum antibodies and the comparison of the IFA and ELISA showed: a) in P. falciparum prime infected patient the sensitivity was 71% for both tests; b) in P. vivax prime infected patients the sensitivity was 40% for both tests; c) in non prime infected patients with P. falciparum malaria the sensitivity was 100% for both tests; d) in non prime infected patients with P. vivax malaria the sensitivity was 85% for ELISA and 92% for IFA; e) in patients with P. vivax and P. falciparum malaria the sensitivity was 100% for both tests. The specificity was 95% for ELISA and 100% for IFA in non malaria individuals. The results showed that the ELISA test could be an alternative for IFA for IgG antibodies in the serology of malaria.
publishDate 1988
dc.date.none.fl_str_mv 1988-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28578
url https://www.revistas.usp.br/rimtsp/article/view/28578
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28578/30431
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 3 (1988); 137-146
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 3 (1988); 137-146
Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 3 (1988); 137-146
1678-9946
0036-4665
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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