Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28578 |
Resumo: | The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtained when sera were diluted in PBS containing 1% Tween-80 and the slides, containing the antigenic preparation were fixed in cold acetone or stabilized on dried air with silica. The ELISA test was standardized for P. falciparum antibodies and the comparison of the IFA and ELISA showed: a) in P. falciparum prime infected patient the sensitivity was 71% for both tests; b) in P. vivax prime infected patients the sensitivity was 40% for both tests; c) in non prime infected patients with P. falciparum malaria the sensitivity was 100% for both tests; d) in non prime infected patients with P. vivax malaria the sensitivity was 85% for ELISA and 92% for IFA; e) in patients with P. vivax and P. falciparum malaria the sensitivity was 100% for both tests. The specificity was 95% for ELISA and 100% for IFA in non malaria individuals. The results showed that the ELISA test could be an alternative for IFA for IgG antibodies in the serology of malaria. |
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Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys Malária humana: padronização e optimização de testes sorológicos para diagnóstico individual e inquéritos soroepidemiológicos MaláriaIFITeste imunoenzimático ELISA The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtained when sera were diluted in PBS containing 1% Tween-80 and the slides, containing the antigenic preparation were fixed in cold acetone or stabilized on dried air with silica. The ELISA test was standardized for P. falciparum antibodies and the comparison of the IFA and ELISA showed: a) in P. falciparum prime infected patient the sensitivity was 71% for both tests; b) in P. vivax prime infected patients the sensitivity was 40% for both tests; c) in non prime infected patients with P. falciparum malaria the sensitivity was 100% for both tests; d) in non prime infected patients with P. vivax malaria the sensitivity was 85% for ELISA and 92% for IFA; e) in patients with P. vivax and P. falciparum malaria the sensitivity was 100% for both tests. The specificity was 95% for ELISA and 100% for IFA in non malaria individuals. The results showed that the ELISA test could be an alternative for IFA for IgG antibodies in the serology of malaria. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1988-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28578Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 3 (1988); 137-146 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 3 (1988); 137-146 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 3 (1988); 137-146 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28578/30431Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFerreira, Antonio WalterSanchez, Maria Carmen Arroyo2012-07-02T01:04:53Zoai:revistas.usp.br:article/28578Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:18.920854Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys Malária humana: padronização e optimização de testes sorológicos para diagnóstico individual e inquéritos soroepidemiológicos |
title |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys |
spellingShingle |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys Ferreira, Antonio Walter Malária IFI Teste imunoenzimático ELISA |
title_short |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys |
title_full |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys |
title_fullStr |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys |
title_full_unstemmed |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys |
title_sort |
Human malaria: standardization of serologic tests for individual diagnostic and seroepidemiologic surveys |
author |
Ferreira, Antonio Walter |
author_facet |
Ferreira, Antonio Walter Sanchez, Maria Carmen Arroyo |
author_role |
author |
author2 |
Sanchez, Maria Carmen Arroyo |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Ferreira, Antonio Walter Sanchez, Maria Carmen Arroyo |
dc.subject.por.fl_str_mv |
Malária IFI Teste imunoenzimático ELISA |
topic |
Malária IFI Teste imunoenzimático ELISA |
description |
The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtained when sera were diluted in PBS containing 1% Tween-80 and the slides, containing the antigenic preparation were fixed in cold acetone or stabilized on dried air with silica. The ELISA test was standardized for P. falciparum antibodies and the comparison of the IFA and ELISA showed: a) in P. falciparum prime infected patient the sensitivity was 71% for both tests; b) in P. vivax prime infected patients the sensitivity was 40% for both tests; c) in non prime infected patients with P. falciparum malaria the sensitivity was 100% for both tests; d) in non prime infected patients with P. vivax malaria the sensitivity was 85% for ELISA and 92% for IFA; e) in patients with P. vivax and P. falciparum malaria the sensitivity was 100% for both tests. The specificity was 95% for ELISA and 100% for IFA in non malaria individuals. The results showed that the ELISA test could be an alternative for IFA for IgG antibodies in the serology of malaria. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28578 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28578 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28578/30431 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 3 (1988); 137-146 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 3 (1988); 137-146 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 3 (1988); 137-146 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951637755101184 |