Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi

Detalhes bibliográficos
Autor(a) principal: Vilani-Moreno, Fátima Regina
Data de Publicação: 2007
Outros Autores: Mozer, Érika, Sene, Ana Márcia Guedes de, Ferasçoli, Margarete de Oliveira, Pereira, Tânia Cristina, Miras, Márcia Garcia, Souza, Gláucia Heloísa de Paula, Belone, Andréa de Faria Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31068
Resumo: Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi.
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spelling Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi In vitro and in situ activation of the complement system by the fungus Lacazia loboi Complement systemLacazia loboiJorge Lobo's diseaseImmunofluorescenceSkin lesions Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi. Considerando que não existe nenhum estudo avaliando a participação do sistema complemento (SC) na doença de Jorge Lobo e sua atividade sobre o fungo Lacazia loboi, realizamos o presente trabalho. Os fungos foram obtidos dos coxins plantares de camundongos BALB/c com índice de viabilidade de 48% e, em seguida, foram incubados com pool de soro inativado de pacientes ou com solução salina estéril (SSE) por 30 min, a 37 ºC. Os tubos foram incubados, por 2 h, com pool de soro AB+ sem inativar, inativado, diluído em EGTA-MgCl2 e EDTA. A viabilidade do L. loboi foi avaliada e a suspensão fúngica foi citocentrifugada. As lâminas foram submetidas à técnica de imunofluorescência empregando o anticorpo anti-C3 humano. Os resultados revelaram que 98% dos fungos ativaram o SC pela via alternativa e que não houve diferença significante na viabilidade do L. loboi após ativação do SC. Em paralelo, cortes histológicos congelados de 11 pacientes foram avaliados quanto à presença de C3 e IgG, pela técnica de imunofluorescência. Foram encontrados depósitos de C3 e de IgG na parede dos fungos em 100% e 91% das lesões avaliadas, respectivamente. Os resultados sugerem que o SC e as imunoglobulinas poderiam contribuir nos mecanismos de defesa do hospedeiro contra o L. loboi. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2007-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31068Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 2 (2007); 97-101 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 2 (2007); 97-101 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 2 (2007); 97-101 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31068/32952Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVilani-Moreno, Fátima ReginaMozer, ÉrikaSene, Ana Márcia Guedes deFerasçoli, Margarete de OliveiraPereira, Tânia CristinaMiras, Márcia GarciaSouza, Gláucia Heloísa de PaulaBelone, Andréa de Faria Fernandes2012-07-07T19:00:09Zoai:revistas.usp.br:article/31068Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:46.161722Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
In vitro and in situ activation of the complement system by the fungus Lacazia loboi
title Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
spellingShingle Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
Vilani-Moreno, Fátima Regina
Complement system
Lacazia loboi
Jorge Lobo's disease
Immunofluorescence
Skin lesions
title_short Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
title_full Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
title_fullStr Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
title_full_unstemmed Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
title_sort Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
author Vilani-Moreno, Fátima Regina
author_facet Vilani-Moreno, Fátima Regina
Mozer, Érika
Sene, Ana Márcia Guedes de
Ferasçoli, Margarete de Oliveira
Pereira, Tânia Cristina
Miras, Márcia Garcia
Souza, Gláucia Heloísa de Paula
Belone, Andréa de Faria Fernandes
author_role author
author2 Mozer, Érika
Sene, Ana Márcia Guedes de
Ferasçoli, Margarete de Oliveira
Pereira, Tânia Cristina
Miras, Márcia Garcia
Souza, Gláucia Heloísa de Paula
Belone, Andréa de Faria Fernandes
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vilani-Moreno, Fátima Regina
Mozer, Érika
Sene, Ana Márcia Guedes de
Ferasçoli, Margarete de Oliveira
Pereira, Tânia Cristina
Miras, Márcia Garcia
Souza, Gláucia Heloísa de Paula
Belone, Andréa de Faria Fernandes
dc.subject.por.fl_str_mv Complement system
Lacazia loboi
Jorge Lobo's disease
Immunofluorescence
Skin lesions
topic Complement system
Lacazia loboi
Jorge Lobo's disease
Immunofluorescence
Skin lesions
description Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi.
publishDate 2007
dc.date.none.fl_str_mv 2007-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31068
url https://www.revistas.usp.br/rimtsp/article/view/31068
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31068/32952
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 2 (2007); 97-101
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 2 (2007); 97-101
Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 2 (2007); 97-101
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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