Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31068 |
Resumo: | Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi. |
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Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi In vitro and in situ activation of the complement system by the fungus Lacazia loboi Complement systemLacazia loboiJorge Lobo's diseaseImmunofluorescenceSkin lesions Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi. Considerando que não existe nenhum estudo avaliando a participação do sistema complemento (SC) na doença de Jorge Lobo e sua atividade sobre o fungo Lacazia loboi, realizamos o presente trabalho. Os fungos foram obtidos dos coxins plantares de camundongos BALB/c com índice de viabilidade de 48% e, em seguida, foram incubados com pool de soro inativado de pacientes ou com solução salina estéril (SSE) por 30 min, a 37 ºC. Os tubos foram incubados, por 2 h, com pool de soro AB+ sem inativar, inativado, diluído em EGTA-MgCl2 e EDTA. A viabilidade do L. loboi foi avaliada e a suspensão fúngica foi citocentrifugada. As lâminas foram submetidas à técnica de imunofluorescência empregando o anticorpo anti-C3 humano. Os resultados revelaram que 98% dos fungos ativaram o SC pela via alternativa e que não houve diferença significante na viabilidade do L. loboi após ativação do SC. Em paralelo, cortes histológicos congelados de 11 pacientes foram avaliados quanto à presença de C3 e IgG, pela técnica de imunofluorescência. Foram encontrados depósitos de C3 e de IgG na parede dos fungos em 100% e 91% das lesões avaliadas, respectivamente. Os resultados sugerem que o SC e as imunoglobulinas poderiam contribuir nos mecanismos de defesa do hospedeiro contra o L. loboi. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2007-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31068Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 2 (2007); 97-101 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 2 (2007); 97-101 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 2 (2007); 97-101 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31068/32952Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVilani-Moreno, Fátima ReginaMozer, ÉrikaSene, Ana Márcia Guedes deFerasçoli, Margarete de OliveiraPereira, Tânia CristinaMiras, Márcia GarciaSouza, Gláucia Heloísa de PaulaBelone, Andréa de Faria Fernandes2012-07-07T19:00:09Zoai:revistas.usp.br:article/31068Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:46.161722Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi In vitro and in situ activation of the complement system by the fungus Lacazia loboi |
title |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
spellingShingle |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi Vilani-Moreno, Fátima Regina Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions |
title_short |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_full |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_fullStr |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_full_unstemmed |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_sort |
Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
author |
Vilani-Moreno, Fátima Regina |
author_facet |
Vilani-Moreno, Fátima Regina Mozer, Érika Sene, Ana Márcia Guedes de Ferasçoli, Margarete de Oliveira Pereira, Tânia Cristina Miras, Márcia Garcia Souza, Gláucia Heloísa de Paula Belone, Andréa de Faria Fernandes |
author_role |
author |
author2 |
Mozer, Érika Sene, Ana Márcia Guedes de Ferasçoli, Margarete de Oliveira Pereira, Tânia Cristina Miras, Márcia Garcia Souza, Gláucia Heloísa de Paula Belone, Andréa de Faria Fernandes |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vilani-Moreno, Fátima Regina Mozer, Érika Sene, Ana Márcia Guedes de Ferasçoli, Margarete de Oliveira Pereira, Tânia Cristina Miras, Márcia Garcia Souza, Gláucia Heloísa de Paula Belone, Andréa de Faria Fernandes |
dc.subject.por.fl_str_mv |
Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions |
topic |
Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions |
description |
Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31068 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31068 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31068/32952 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 2 (2007); 97-101 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 2 (2007); 97-101 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 2 (2007); 97-101 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951646205575168 |