Fecal specimens preparation methods for PCR diagnosis of human taeniosis

Detalhes bibliográficos
Autor(a) principal: Nunes, Cáris Maroni
Data de Publicação: 2006
Outros Autores: Lima, Luiz Gustavo Ferraz, Manoel, Camila Santos, Pereira, Rodrigo Norberto, Nakano, Mauro Massaharu, Garcia, José Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30968
Resumo: Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.
id IMT-1_9c214627d90d883eb7ba4b779343b6b3
oai_identifier_str oai:revistas.usp.br:article/30968
network_acronym_str IMT-1
network_name_str Revista do Instituto de Medicina Tropical de São Paulo
repository_id_str
spelling Fecal specimens preparation methods for PCR diagnosis of human taeniosis Métodos de preparação de amostras de fezes para diagnóstico de teníase humana através da PCR Taenia saginataDNA extractionPolymerase chain reaction (PCR)Fecal specimens Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®. Com o intuito de utilizar a Reação em Cadeia pela Polimerase (PCR) como método de diagnóstico diferencial da teníase humana, avaliaram-se alguns protocolos de preparação e extração de DNA de ovos de Taenia saginata presentes em amostras de fezes de paciente naturalmente infectado. O DNA obtido após extração com fenol/clorofórmio/álcool isoamílico ou DNAzol® teve que ser purificado antes da PCR para que fosse possível a amplificação dos fragmentos de 170 pb e 600 pb desejados. Com o kit QIAmp DNA stool mini kit® tal purificação não foi necessária. Os melhores resultados foram observados após o tratamento prévio das amostras com pérolas de vidro, tanto quando da utilização de fenol/clorofórmio/álcool isoamílico, quando de DNAzol® ou QIAmp DNA stool mini kit®. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30968Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 No. 1 (2006); 45-47 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 Núm. 1 (2006); 45-47 Revista do Instituto de Medicina Tropical de São Paulo; v. 48 n. 1 (2006); 45-47 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30968/32852Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessNunes, Cáris MaroniLima, Luiz Gustavo FerrazManoel, Camila SantosPereira, Rodrigo NorbertoNakano, Mauro MassaharuGarcia, José Fernando2015-07-22T18:59:08Zoai:revistas.usp.br:article/30968Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:41.244925Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Fecal specimens preparation methods for PCR diagnosis of human taeniosis
Métodos de preparação de amostras de fezes para diagnóstico de teníase humana através da PCR
title Fecal specimens preparation methods for PCR diagnosis of human taeniosis
spellingShingle Fecal specimens preparation methods for PCR diagnosis of human taeniosis
Nunes, Cáris Maroni
Taenia saginata
DNA extraction
Polymerase chain reaction (PCR)
Fecal specimens
title_short Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_full Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_fullStr Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_full_unstemmed Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_sort Fecal specimens preparation methods for PCR diagnosis of human taeniosis
author Nunes, Cáris Maroni
author_facet Nunes, Cáris Maroni
Lima, Luiz Gustavo Ferraz
Manoel, Camila Santos
Pereira, Rodrigo Norberto
Nakano, Mauro Massaharu
Garcia, José Fernando
author_role author
author2 Lima, Luiz Gustavo Ferraz
Manoel, Camila Santos
Pereira, Rodrigo Norberto
Nakano, Mauro Massaharu
Garcia, José Fernando
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, Cáris Maroni
Lima, Luiz Gustavo Ferraz
Manoel, Camila Santos
Pereira, Rodrigo Norberto
Nakano, Mauro Massaharu
Garcia, José Fernando
dc.subject.por.fl_str_mv Taenia saginata
DNA extraction
Polymerase chain reaction (PCR)
Fecal specimens
topic Taenia saginata
DNA extraction
Polymerase chain reaction (PCR)
Fecal specimens
description Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30968
url https://www.revistas.usp.br/rimtsp/article/view/30968
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30968/32852
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 No. 1 (2006); 45-47
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 48 Núm. 1 (2006); 45-47
Revista do Instituto de Medicina Tropical de São Paulo; v. 48 n. 1 (2006); 45-47
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951645667655680

Registros relacionados