Fecal specimens preparation methods for PCR diagnosis of human taeniosis

Detalhes bibliográficos
Autor(a) principal: Nunes, Cáris Maroni [UNESP]
Data de Publicação: 2006
Outros Autores: Lima, Luiz Gustavo Ferraz [UNESP], Manoel, Camila Santos [UNESP], Pereira, Rodrigo Norberto [UNESP], Nakano, Mauro Massaharu [UNESP], Garcia, José Fernando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/3527608788.ch6
http://hdl.handle.net/11449/224729
Resumo: Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.
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spelling Fecal specimens preparation methods for PCR diagnosis of human taeniosisDNA extractionFecal specimensPolymerase chain reaction (PCR)Taenia saginataSample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.UNESP Campus Araçatuba Departamento de Apoio, Produção e Saúde Animal, Araçatuba, SPUNESP Campus Araçatuba Departamento de Apoio, Produção e Saúde Animal, Araçatuba, SPUniversidade Estadual Paulista (UNESP)Nunes, Cáris Maroni [UNESP]Lima, Luiz Gustavo Ferraz [UNESP]Manoel, Camila Santos [UNESP]Pereira, Rodrigo Norberto [UNESP]Nakano, Mauro Massaharu [UNESP]Garcia, José Fernando [UNESP]2022-04-28T20:07:38Z2022-04-28T20:07:38Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article45-47http://dx.doi.org/10.1002/3527608788.ch6Revista do Instituto de Medicina Tropical de Sao Paulo, v. 48, n. 1, p. 45-47, 2006.0036-46651678-9946http://hdl.handle.net/11449/22472910.1002/3527608788.ch62-s2.0-33645031866Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de Sao Pauloinfo:eu-repo/semantics/openAccess2024-09-04T19:16:07Zoai:repositorio.unesp.br:11449/224729Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title Fecal specimens preparation methods for PCR diagnosis of human taeniosis
spellingShingle Fecal specimens preparation methods for PCR diagnosis of human taeniosis
Nunes, Cáris Maroni [UNESP]
DNA extraction
Fecal specimens
Polymerase chain reaction (PCR)
Taenia saginata
title_short Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_full Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_fullStr Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_full_unstemmed Fecal specimens preparation methods for PCR diagnosis of human taeniosis
title_sort Fecal specimens preparation methods for PCR diagnosis of human taeniosis
author Nunes, Cáris Maroni [UNESP]
author_facet Nunes, Cáris Maroni [UNESP]
Lima, Luiz Gustavo Ferraz [UNESP]
Manoel, Camila Santos [UNESP]
Pereira, Rodrigo Norberto [UNESP]
Nakano, Mauro Massaharu [UNESP]
Garcia, José Fernando [UNESP]
author_role author
author2 Lima, Luiz Gustavo Ferraz [UNESP]
Manoel, Camila Santos [UNESP]
Pereira, Rodrigo Norberto [UNESP]
Nakano, Mauro Massaharu [UNESP]
Garcia, José Fernando [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Nunes, Cáris Maroni [UNESP]
Lima, Luiz Gustavo Ferraz [UNESP]
Manoel, Camila Santos [UNESP]
Pereira, Rodrigo Norberto [UNESP]
Nakano, Mauro Massaharu [UNESP]
Garcia, José Fernando [UNESP]
dc.subject.por.fl_str_mv DNA extraction
Fecal specimens
Polymerase chain reaction (PCR)
Taenia saginata
topic DNA extraction
Fecal specimens
Polymerase chain reaction (PCR)
Taenia saginata
description Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
2022-04-28T20:07:38Z
2022-04-28T20:07:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/3527608788.ch6
Revista do Instituto de Medicina Tropical de Sao Paulo, v. 48, n. 1, p. 45-47, 2006.
0036-4665
1678-9946
http://hdl.handle.net/11449/224729
10.1002/3527608788.ch6
2-s2.0-33645031866
url http://dx.doi.org/10.1002/3527608788.ch6
http://hdl.handle.net/11449/224729
identifier_str_mv Revista do Instituto de Medicina Tropical de Sao Paulo, v. 48, n. 1, p. 45-47, 2006.
0036-4665
1678-9946
10.1002/3527608788.ch6
2-s2.0-33645031866
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de Sao Paulo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 45-47
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021418745724928

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