A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp

Detalhes bibliográficos
Autor(a) principal: Hernández, Francisco
Data de Publicação: 1996
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29370
Resumo: Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.
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spelling A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp Campylobactertechnical assayMicroaerobiosis^i1^spoul Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories. Se inocularon por duplicado 138 muestras de heces de pollos en agar de Blaser. Un juego de las placas se incubó en jarras con sobres generadores de microaerobiosis (CampyPak). Las otras placas se incubaron en bolsas plásticas tipo "Zip-lock" (7 x 8 pulgadas) y la atmósfera microaerofílica se generó inflando la bolsa luego de retener la respiración por 20 seg, desinflándola e inflándola nuevamente (4 veces) y finalmente sellándola. Se aisló Campylobacter de 127 (96.2%) de las muestras incubadas en la jarra y de 129 (98%) de los especimenes incubados en la bolsa. La metodologia propuesta ofrece una posibilidad económica para los investigadores concientes de los costos de operación. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1996-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29370Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 3 (1996); 241-242 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 3 (1996); 241-242 Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 3 (1996); 241-242 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29370/31227Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessHernández, Francisco2012-07-02T01:43:41Zoai:revistas.usp.br:article/29370Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:07.082113Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp
title A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
spellingShingle A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
Hernández, Francisco
Campylobacter
technical assay
Microaerobiosis^i1^spoul
title_short A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
title_full A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
title_fullStr A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
title_full_unstemmed A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
title_sort A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
author Hernández, Francisco
author_facet Hernández, Francisco
author_role author
dc.contributor.author.fl_str_mv Hernández, Francisco
dc.subject.por.fl_str_mv Campylobacter
technical assay
Microaerobiosis^i1^spoul
topic Campylobacter
technical assay
Microaerobiosis^i1^spoul
description Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.
publishDate 1996
dc.date.none.fl_str_mv 1996-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29370
url https://www.revistas.usp.br/rimtsp/article/view/29370
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29370/31227
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 3 (1996); 241-242
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 3 (1996); 241-242
Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 3 (1996); 241-242
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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