A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29370 |
Resumo: | Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories. |
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A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp Campylobactertechnical assayMicroaerobiosis^i1^spoul Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories. Se inocularon por duplicado 138 muestras de heces de pollos en agar de Blaser. Un juego de las placas se incubó en jarras con sobres generadores de microaerobiosis (CampyPak). Las otras placas se incubaron en bolsas plásticas tipo "Zip-lock" (7 x 8 pulgadas) y la atmósfera microaerofílica se generó inflando la bolsa luego de retener la respiración por 20 seg, desinflándola e inflándola nuevamente (4 veces) y finalmente sellándola. Se aisló Campylobacter de 127 (96.2%) de las muestras incubadas en la jarra y de 129 (98%) de los especimenes incubados en la bolsa. La metodologia propuesta ofrece una posibilidad económica para los investigadores concientes de los costos de operación. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1996-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29370Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 3 (1996); 241-242 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 3 (1996); 241-242 Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 3 (1996); 241-242 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29370/31227Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessHernández, Francisco2012-07-02T01:43:41Zoai:revistas.usp.br:article/29370Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:07.082113Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp |
title |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp |
spellingShingle |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp Hernández, Francisco Campylobacter technical assay Microaerobiosis^i1^spoul |
title_short |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp |
title_full |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp |
title_fullStr |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp |
title_full_unstemmed |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp |
title_sort |
A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp |
author |
Hernández, Francisco |
author_facet |
Hernández, Francisco |
author_role |
author |
dc.contributor.author.fl_str_mv |
Hernández, Francisco |
dc.subject.por.fl_str_mv |
Campylobacter technical assay Microaerobiosis^i1^spoul |
topic |
Campylobacter technical assay Microaerobiosis^i1^spoul |
description |
Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29370 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29370 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29370/31227 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 3 (1996); 241-242 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 3 (1996); 241-242 Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 3 (1996); 241-242 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951642249297920 |