First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31447 |
Resumo: | We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized. |
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First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 Primeiro isolamento de Dengue 4 no Estado de São Paulo, Brasil, 2011 Dengue 4Virus isolationRT-PCR We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized. Relatamos o primeiro isolamento do vírus Dengue 4 (DENV-4) no Estado de São Paulo, de dois pacientes residentes em São José do Rio Preto e Paulo de Faria, ambos municípios localizados na região Noroeste do Estado. O isolamento do vírus foi realizado em clone C6/36, linhagem de células de Aedes albopictus seguido por imunofluorescência indireta, realizada com anticorpos monoclonais tipo específicos, que apresentou reação positiva para DENV-4. Os resultados foram confirmados por testes de Nested RT-PCR e RT-PCR em Tempo Real. A introdução do DENV-4 no país, com uma população suscetível a esse vírus e que já convive com a transmissão de outros três sorotipos, aumenta a possibilidade da ocorrência de casos mais graves da doença. Deve ser enfatizada a importância da detecção precoce de casos de dengue, antes que ocorra a propagação do vírus e que surtos importantes aconteçam. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31447Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 No. 1 (2012); 49-51 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 Núm. 1 (2012); 49-51 Revista do Instituto de Medicina Tropical de São Paulo; v. 54 n. 1 (2012); 49-51 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31447/33332Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessRocco, Iray MariaSilveira, Vivian ReginaMaeda, Adriana YurikaSilva, Sarai Joaquim dos SantosSpenassatto, CarineBISORDI, IvaniSuzuki, Akemi2012-07-07T19:44:53Zoai:revistas.usp.br:article/31447Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:07.258237Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 Primeiro isolamento de Dengue 4 no Estado de São Paulo, Brasil, 2011 |
title |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 |
spellingShingle |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 Rocco, Iray Maria Dengue 4 Virus isolation RT-PCR |
title_short |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 |
title_full |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 |
title_fullStr |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 |
title_full_unstemmed |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 |
title_sort |
First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 |
author |
Rocco, Iray Maria |
author_facet |
Rocco, Iray Maria Silveira, Vivian Regina Maeda, Adriana Yurika Silva, Sarai Joaquim dos Santos Spenassatto, Carine BISORDI, Ivani Suzuki, Akemi |
author_role |
author |
author2 |
Silveira, Vivian Regina Maeda, Adriana Yurika Silva, Sarai Joaquim dos Santos Spenassatto, Carine BISORDI, Ivani Suzuki, Akemi |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rocco, Iray Maria Silveira, Vivian Regina Maeda, Adriana Yurika Silva, Sarai Joaquim dos Santos Spenassatto, Carine BISORDI, Ivani Suzuki, Akemi |
dc.subject.por.fl_str_mv |
Dengue 4 Virus isolation RT-PCR |
topic |
Dengue 4 Virus isolation RT-PCR |
description |
We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31447 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31447 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31447/33332 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 No. 1 (2012); 49-51 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 Núm. 1 (2012); 49-51 Revista do Instituto de Medicina Tropical de São Paulo; v. 54 n. 1 (2012); 49-51 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951648425410560 |