First isolation of dengue 4 in the state of São Paulo, Brazil, 2011

Detalhes bibliográficos
Autor(a) principal: Rocco, Iray Maria
Data de Publicação: 2012
Outros Autores: Silveira, Vivian Regina, Maeda, Adriana Yurika, Silva, Sarai Joaquim dos Santos, Spenassatto, Carine, BISORDI, Ivani, Suzuki, Akemi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31447
Resumo: We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized.
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spelling First isolation of dengue 4 in the state of São Paulo, Brazil, 2011 Primeiro isolamento de Dengue 4 no Estado de São Paulo, Brasil, 2011 Dengue 4Virus isolationRT-PCR We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized. Relatamos o primeiro isolamento do vírus Dengue 4 (DENV-4) no Estado de São Paulo, de dois pacientes residentes em São José do Rio Preto e Paulo de Faria, ambos municípios localizados na região Noroeste do Estado. O isolamento do vírus foi realizado em clone C6/36, linhagem de células de Aedes albopictus seguido por imunofluorescência indireta, realizada com anticorpos monoclonais tipo específicos, que apresentou reação positiva para DENV-4. Os resultados foram confirmados por testes de Nested RT-PCR e RT-PCR em Tempo Real. A introdução do DENV-4 no país, com uma população suscetível a esse vírus e que já convive com a transmissão de outros três sorotipos, aumenta a possibilidade da ocorrência de casos mais graves da doença. Deve ser enfatizada a importância da detecção precoce de casos de dengue, antes que ocorra a propagação do vírus e que surtos importantes aconteçam. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31447Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 No. 1 (2012); 49-51 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 Núm. 1 (2012); 49-51 Revista do Instituto de Medicina Tropical de São Paulo; v. 54 n. 1 (2012); 49-51 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31447/33332Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessRocco, Iray MariaSilveira, Vivian ReginaMaeda, Adriana YurikaSilva, Sarai Joaquim dos SantosSpenassatto, CarineBISORDI, IvaniSuzuki, Akemi2012-07-07T19:44:53Zoai:revistas.usp.br:article/31447Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:07.258237Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
Primeiro isolamento de Dengue 4 no Estado de São Paulo, Brasil, 2011
title First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
spellingShingle First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
Rocco, Iray Maria
Dengue 4
Virus isolation
RT-PCR
title_short First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
title_full First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
title_fullStr First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
title_full_unstemmed First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
title_sort First isolation of dengue 4 in the state of São Paulo, Brazil, 2011
author Rocco, Iray Maria
author_facet Rocco, Iray Maria
Silveira, Vivian Regina
Maeda, Adriana Yurika
Silva, Sarai Joaquim dos Santos
Spenassatto, Carine
BISORDI, Ivani
Suzuki, Akemi
author_role author
author2 Silveira, Vivian Regina
Maeda, Adriana Yurika
Silva, Sarai Joaquim dos Santos
Spenassatto, Carine
BISORDI, Ivani
Suzuki, Akemi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rocco, Iray Maria
Silveira, Vivian Regina
Maeda, Adriana Yurika
Silva, Sarai Joaquim dos Santos
Spenassatto, Carine
BISORDI, Ivani
Suzuki, Akemi
dc.subject.por.fl_str_mv Dengue 4
Virus isolation
RT-PCR
topic Dengue 4
Virus isolation
RT-PCR
description We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31447
url https://www.revistas.usp.br/rimtsp/article/view/31447
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31447/33332
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 No. 1 (2012); 49-51
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 Núm. 1 (2012); 49-51
Revista do Instituto de Medicina Tropical de São Paulo; v. 54 n. 1 (2012); 49-51
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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