Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Carla Grasso
Data de Publicação: 2020
Outros Autores: Luchs, Adriana, Durigon, Edison Luiz, Oliveira, Danielle Bruna Leal de, Silva, Vanessa Barbosa da, Mello, Ralyria Melyria, Afonso, Ana Maria Sardinha, Oliveira, Maria Isabel de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/173403
Resumo: Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positiveHCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.
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spelling Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018Congenital cytomegalovirus infectionReal-time PCRSurveillanceDNA sequencingHuman cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positiveHCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/17340310.1590/s1678-9946202062054Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e541678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/173403/162603https://www.revistas.usp.br/rimtsp/article/view/173403/162604Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFigueiredo, Carla GrassoLuchs, AdrianaDurigon, Edison LuizOliveira, Danielle Bruna Leal deSilva, Vanessa Barbosa daMello, Ralyria MelyriaAfonso, Ana Maria SardinhaOliveira, Maria Isabel de2020-10-26T17:39:25Zoai:revistas.usp.br:article/173403Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:53.369237Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
title Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
spellingShingle Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
Figueiredo, Carla Grasso
Congenital cytomegalovirus infection
Real-time PCR
Surveillance
DNA sequencing
title_short Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
title_full Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
title_fullStr Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
title_full_unstemmed Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
title_sort Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
author Figueiredo, Carla Grasso
author_facet Figueiredo, Carla Grasso
Luchs, Adriana
Durigon, Edison Luiz
Oliveira, Danielle Bruna Leal de
Silva, Vanessa Barbosa da
Mello, Ralyria Melyria
Afonso, Ana Maria Sardinha
Oliveira, Maria Isabel de
author_role author
author2 Luchs, Adriana
Durigon, Edison Luiz
Oliveira, Danielle Bruna Leal de
Silva, Vanessa Barbosa da
Mello, Ralyria Melyria
Afonso, Ana Maria Sardinha
Oliveira, Maria Isabel de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figueiredo, Carla Grasso
Luchs, Adriana
Durigon, Edison Luiz
Oliveira, Danielle Bruna Leal de
Silva, Vanessa Barbosa da
Mello, Ralyria Melyria
Afonso, Ana Maria Sardinha
Oliveira, Maria Isabel de
dc.subject.por.fl_str_mv Congenital cytomegalovirus infection
Real-time PCR
Surveillance
DNA sequencing
topic Congenital cytomegalovirus infection
Real-time PCR
Surveillance
DNA sequencing
description Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positiveHCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/173403
10.1590/s1678-9946202062054
url https://www.revistas.usp.br/rimtsp/article/view/173403
identifier_str_mv 10.1590/s1678-9946202062054
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/173403/162603
https://www.revistas.usp.br/rimtsp/article/view/173403/162604
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54
Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e54
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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