Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/173403 |
Resumo: | Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positiveHCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections. |
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Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018Congenital cytomegalovirus infectionReal-time PCRSurveillanceDNA sequencingHuman cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positiveHCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/17340310.1590/s1678-9946202062054Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e541678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/173403/162603https://www.revistas.usp.br/rimtsp/article/view/173403/162604Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFigueiredo, Carla GrassoLuchs, AdrianaDurigon, Edison LuizOliveira, Danielle Bruna Leal deSilva, Vanessa Barbosa daMello, Ralyria MelyriaAfonso, Ana Maria SardinhaOliveira, Maria Isabel de2020-10-26T17:39:25Zoai:revistas.usp.br:article/173403Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:53.369237Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
title |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
spellingShingle |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 Figueiredo, Carla Grasso Congenital cytomegalovirus infection Real-time PCR Surveillance DNA sequencing |
title_short |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
title_full |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
title_fullStr |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
title_full_unstemmed |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
title_sort |
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018 |
author |
Figueiredo, Carla Grasso |
author_facet |
Figueiredo, Carla Grasso Luchs, Adriana Durigon, Edison Luiz Oliveira, Danielle Bruna Leal de Silva, Vanessa Barbosa da Mello, Ralyria Melyria Afonso, Ana Maria Sardinha Oliveira, Maria Isabel de |
author_role |
author |
author2 |
Luchs, Adriana Durigon, Edison Luiz Oliveira, Danielle Bruna Leal de Silva, Vanessa Barbosa da Mello, Ralyria Melyria Afonso, Ana Maria Sardinha Oliveira, Maria Isabel de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Figueiredo, Carla Grasso Luchs, Adriana Durigon, Edison Luiz Oliveira, Danielle Bruna Leal de Silva, Vanessa Barbosa da Mello, Ralyria Melyria Afonso, Ana Maria Sardinha Oliveira, Maria Isabel de |
dc.subject.por.fl_str_mv |
Congenital cytomegalovirus infection Real-time PCR Surveillance DNA sequencing |
topic |
Congenital cytomegalovirus infection Real-time PCR Surveillance DNA sequencing |
description |
Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positiveHCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/173403 10.1590/s1678-9946202062054 |
url |
https://www.revistas.usp.br/rimtsp/article/view/173403 |
identifier_str_mv |
10.1590/s1678-9946202062054 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/173403/162603 https://www.revistas.usp.br/rimtsp/article/view/173403/162604 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e54 Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e54 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951652722475008 |