Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil

Detalhes bibliográficos
Autor(a) principal: AVILA-CAMPOS, Mario J.
Data de Publicação: 2002
Outros Autores: VELÁSQUEZ-MELÉNDEZ, Gustavo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30588
Resumo: A. actinomycetemcomitans, B. forsythus, P. gingivalis, C. rectus, E. corrodens, P. intermedia, F. nucleatum, and T. denticola were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. PCR products from each species showed a specific band and could be used to identify periodontal organisms from clinical specimens. Identical negative or positive results between PCR and culture occurred in 66% (A. actinomycetemcomitans) to 93% (F. nucleatum) of the samples. PCR detection odds ratio values for A. actinomycetemcomitans, B. forsythus, C. rectus, E. corrodens, P. intermedia, and T. denticola were significantly associated with disease having a higher OR values for B. forsythus (2.97, 95% CI 1.88 - 4.70). Cultures showed that A. actinomycetemcomitans, B. forsythus and P. intermedia were associated with periodontitis, however, P. gingivalis, C. rectus, E. corrodens and F. nucleatum were not significantly associated with the disease.
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spelling Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil Prevalência de periodontopatógenos em pacientes com doença periodontal e indivíduos sadios de São Paulo, SP, Brasil Periodontal pathogensPeriodontal diseasePCR A. actinomycetemcomitans, B. forsythus, P. gingivalis, C. rectus, E. corrodens, P. intermedia, F. nucleatum, and T. denticola were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. PCR products from each species showed a specific band and could be used to identify periodontal organisms from clinical specimens. Identical negative or positive results between PCR and culture occurred in 66% (A. actinomycetemcomitans) to 93% (F. nucleatum) of the samples. PCR detection odds ratio values for A. actinomycetemcomitans, B. forsythus, C. rectus, E. corrodens, P. intermedia, and T. denticola were significantly associated with disease having a higher OR values for B. forsythus (2.97, 95% CI 1.88 - 4.70). Cultures showed that A. actinomycetemcomitans, B. forsythus and P. intermedia were associated with periodontitis, however, P. gingivalis, C. rectus, E. corrodens and F. nucleatum were not significantly associated with the disease. Cepas de A. actinomycetemcomitans, B. forsythus, P. gingivalis, C. rectus, E. corrodens, P. intermedia, F. nucleatum e T. denticola foram identificadas da placa subgengival de 50 pacientes com doença periodontal e 50 indivíduos sadios. Os produtos de PCR de cada espécie microbiana mostraram bandas específicas, favorecendo a identificação direta de bactérias periodontais de espécimes clínicos. Resultados negativos ou positivos idênticos entre PCR e a cultura ocorreram de 66% (A. actinomycetemcomitans) a 93% (F. nucleatum) das amostras. A detecção de A. actinomycetemcomitans, B. forsythus, C. rectus, E. corrodens, P. intermedia e T. denticola por PCR foi significativamente associada com a doença, mostrando valores altos de odds ratio (OR) para B. forsythus (2,97, 95% CI 1,88 - 4,70). Através da cultura foi observada a associação de A. actinomycetemcomitans, B. forsythus e P. intermedia com a periodontite, entretanto, P. gingivalis, C. rectus, E. corrodens e F. nucleatum não foram significativamente relacionados à doença. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2002-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30588Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 1 (2002); 1-5 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 1 (2002); 1-5 Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 1 (2002); 1-5 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30588/32472Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessAVILA-CAMPOS, Mario J.VELÁSQUEZ-MELÉNDEZ, Gustavo2012-07-07T17:49:59Zoai:revistas.usp.br:article/30588Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:22.259123Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
Prevalência de periodontopatógenos em pacientes com doença periodontal e indivíduos sadios de São Paulo, SP, Brasil
title Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
spellingShingle Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
AVILA-CAMPOS, Mario J.
Periodontal pathogens
Periodontal disease
PCR
title_short Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
title_full Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
title_fullStr Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
title_full_unstemmed Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
title_sort Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil
author AVILA-CAMPOS, Mario J.
author_facet AVILA-CAMPOS, Mario J.
VELÁSQUEZ-MELÉNDEZ, Gustavo
author_role author
author2 VELÁSQUEZ-MELÉNDEZ, Gustavo
author2_role author
dc.contributor.author.fl_str_mv AVILA-CAMPOS, Mario J.
VELÁSQUEZ-MELÉNDEZ, Gustavo
dc.subject.por.fl_str_mv Periodontal pathogens
Periodontal disease
PCR
topic Periodontal pathogens
Periodontal disease
PCR
description A. actinomycetemcomitans, B. forsythus, P. gingivalis, C. rectus, E. corrodens, P. intermedia, F. nucleatum, and T. denticola were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. PCR products from each species showed a specific band and could be used to identify periodontal organisms from clinical specimens. Identical negative or positive results between PCR and culture occurred in 66% (A. actinomycetemcomitans) to 93% (F. nucleatum) of the samples. PCR detection odds ratio values for A. actinomycetemcomitans, B. forsythus, C. rectus, E. corrodens, P. intermedia, and T. denticola were significantly associated with disease having a higher OR values for B. forsythus (2.97, 95% CI 1.88 - 4.70). Cultures showed that A. actinomycetemcomitans, B. forsythus and P. intermedia were associated with periodontitis, however, P. gingivalis, C. rectus, E. corrodens and F. nucleatum were not significantly associated with the disease.
publishDate 2002
dc.date.none.fl_str_mv 2002-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30588
url https://www.revistas.usp.br/rimtsp/article/view/30588
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30588/32472
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 1 (2002); 1-5
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 1 (2002); 1-5
Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 1 (2002); 1-5
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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