Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30428 |
Resumo: | We used a molecular method and demonstrated that treatment of the chronic human Trypanosoma cruzi infections with nitroderivatives did not lead to parasitological cure. Seventeen treated and 17 untreated chronic Chagas' disease patients, with at least two out of three positive serologic assays for the infection, and 17 control subjects formed the study groups. PCR assays with nested sets of T. cruzi DNA primers monitored the efficacy of treatment. The amplification products were hybridized to their complementary internal sequences. Untreated and treated Chagas' disease patients yielded PCR amplification products with T. cruzi nuclear DNA primers. Competitive PCR was conducted to determine the quantity of parasites in the blood and revealed < 1 to 75 T. cruzi/ml in untreated (means 25.83 ± 26.32) and < 1 to 36 T. cruzi/ml in treated (means 6.45 ± 9.28) Chagas' disease patients. The difference between the means was not statistically significant. These findings reveal a need for precise definition of the role of treatment of chronic Chagas' disease patients with nitrofuran and nitroimidazole compounds. |
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Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives Persistência das infecções em pacientes chagásicos crônicos tratados com nitroderivados anti-Trypanosoma cruzi Trypanosoma crChagas' diseTreatmNifurtiBenznidaz We used a molecular method and demonstrated that treatment of the chronic human Trypanosoma cruzi infections with nitroderivatives did not lead to parasitological cure. Seventeen treated and 17 untreated chronic Chagas' disease patients, with at least two out of three positive serologic assays for the infection, and 17 control subjects formed the study groups. PCR assays with nested sets of T. cruzi DNA primers monitored the efficacy of treatment. The amplification products were hybridized to their complementary internal sequences. Untreated and treated Chagas' disease patients yielded PCR amplification products with T. cruzi nuclear DNA primers. Competitive PCR was conducted to determine the quantity of parasites in the blood and revealed < 1 to 75 T. cruzi/ml in untreated (means 25.83 ± 26.32) and < 1 to 36 T. cruzi/ml in treated (means 6.45 ± 9.28) Chagas' disease patients. The difference between the means was not statistically significant. These findings reveal a need for precise definition of the role of treatment of chronic Chagas' disease patients with nitrofuran and nitroimidazole compounds. Usamos um método molecular e demonstramos que o tratamento de infecções crônicas pelo Trypanosoma cruzi com nitroderivados, que se mostraram eficientes em diminuir parasitemias nas infecções agudas, não produziu cura parasitológica. Trinta e quatro chagásicos crônicos, com pelo menos dois entre três testes sorológicos positivos para a infecção, e 17 controles com testes sorológicos negativos formaram os grupos de estudo. Os testes de PCR com primers de DNA de T. cruzi monitoraram a eficácia do tratamento. Foram obtidos produtos de PCR a partir de DNA de pacientes chagásicos tratados e não-tratados, com primers de DNA nuclear de T. cruzi. Os produtos amplificados hibridizaram com suas sequências internas e complementares. A técnica de PCR competitiva foi usada para quantificar o número de parasitos no sangue e revelou < 1 a 75 T. cruzi/ml em chagásicos não-tratados (média 25,83 ± 26,32) e < 1 a 36 T. cruzi/ml em chagásicos tratados (média 6,45 ± 9,28). A diferença entre as médias não foi estatisticamente significativa. O resultado mostra que o tratamento da doença de Chagas crônica com drogas nitroderivadas é insatisfatório. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30428Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 3 (2000); 157-161 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 3 (2000); 157-161 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 3 (2000); 157-161 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30428/32312Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessBRAGA, M. SocorroLAURIA-PIRES, LianaARGAÑARAZ, Enrique R.NASCIMENTO, Rubens J.TEIXEIRA, Antonio R. L.2012-07-07T09:34:49Zoai:revistas.usp.br:article/30428Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:11.981819Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives Persistência das infecções em pacientes chagásicos crônicos tratados com nitroderivados anti-Trypanosoma cruzi |
title |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives |
spellingShingle |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives BRAGA, M. Socorro Trypanosoma cr Chagas' dise Treatm Nifurti Benznidaz |
title_short |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives |
title_full |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives |
title_fullStr |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives |
title_full_unstemmed |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives |
title_sort |
Persistent infections in chronic Chagas' disease patients treated with anti-Trypanosoma cruzi nitroderivatives |
author |
BRAGA, M. Socorro |
author_facet |
BRAGA, M. Socorro LAURIA-PIRES, Liana ARGAÑARAZ, Enrique R. NASCIMENTO, Rubens J. TEIXEIRA, Antonio R. L. |
author_role |
author |
author2 |
LAURIA-PIRES, Liana ARGAÑARAZ, Enrique R. NASCIMENTO, Rubens J. TEIXEIRA, Antonio R. L. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
BRAGA, M. Socorro LAURIA-PIRES, Liana ARGAÑARAZ, Enrique R. NASCIMENTO, Rubens J. TEIXEIRA, Antonio R. L. |
dc.subject.por.fl_str_mv |
Trypanosoma cr Chagas' dise Treatm Nifurti Benznidaz |
topic |
Trypanosoma cr Chagas' dise Treatm Nifurti Benznidaz |
description |
We used a molecular method and demonstrated that treatment of the chronic human Trypanosoma cruzi infections with nitroderivatives did not lead to parasitological cure. Seventeen treated and 17 untreated chronic Chagas' disease patients, with at least two out of three positive serologic assays for the infection, and 17 control subjects formed the study groups. PCR assays with nested sets of T. cruzi DNA primers monitored the efficacy of treatment. The amplification products were hybridized to their complementary internal sequences. Untreated and treated Chagas' disease patients yielded PCR amplification products with T. cruzi nuclear DNA primers. Competitive PCR was conducted to determine the quantity of parasites in the blood and revealed < 1 to 75 T. cruzi/ml in untreated (means 25.83 ± 26.32) and < 1 to 36 T. cruzi/ml in treated (means 6.45 ± 9.28) Chagas' disease patients. The difference between the means was not statistically significant. These findings reveal a need for precise definition of the role of treatment of chronic Chagas' disease patients with nitrofuran and nitroimidazole compounds. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30428 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30428/32312 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 3 (2000); 157-161 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 3 (2000); 157-161 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 3 (2000); 157-161 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951642382467072 |