P System antigenic determiners expression in Ascaris lumbricoides

Detalhes bibliográficos
Autor(a) principal: Ponce De León, Patricia
Data de Publicação: 2003
Outros Autores: Valverde, Juana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30672
Resumo: The P System antigens have been detected in numerous parasites, bacterias and viruses, nevertheless the clinical significance is still unknown. The aim was to study the presence of P1 antigenic determiners in A. lumbricoides extracts by means of the use of 6 different monoclonal antibodies of well-known concentrations and Ig class. We worked with 14 A. lumbricoides extracts. Inhibition Agglutination Test was made in a bromelin enzymatic medium and 4 ºC temperature. Titre, Score and Sensitivity Parameter were determined for each monoclonal antibody against red cells suspension used as revealing system. Ten extracts inhibited the agglutination of all anti P1 monoclonal antibodies. The 4 remaining extracts only inhibited the agglutination of some of them. It is demonstrated that the extracts have P1 activity. This activity is independent of titre, Score, Sensitivity Parameter, concentration and Ig class and it depends on the epitope at which the monoclonal antibody is directed.
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spelling P System antigenic determiners expression in Ascaris lumbricoides Presencia de determinantes antigénicas P1 en Ascaris lumbricoides Ascaris lumbricoidesP System The P System antigens have been detected in numerous parasites, bacterias and viruses, nevertheless the clinical significance is still unknown. The aim was to study the presence of P1 antigenic determiners in A. lumbricoides extracts by means of the use of 6 different monoclonal antibodies of well-known concentrations and Ig class. We worked with 14 A. lumbricoides extracts. Inhibition Agglutination Test was made in a bromelin enzymatic medium and 4 ºC temperature. Titre, Score and Sensitivity Parameter were determined for each monoclonal antibody against red cells suspension used as revealing system. Ten extracts inhibited the agglutination of all anti P1 monoclonal antibodies. The 4 remaining extracts only inhibited the agglutination of some of them. It is demonstrated that the extracts have P1 activity. This activity is independent of titre, Score, Sensitivity Parameter, concentration and Ig class and it depends on the epitope at which the monoclonal antibody is directed. Los antígenos del Sistema P han sido detectados en numerosos parásitos, bacterias y virus, aunque todavía se desconoce su significado clínico. El objetivo fue estudiar la presencia de determinantes antigénicas P1 en extractos de A. lumbricoides mediante el uso de 6 anticuerpos monoclonales de concentraciones y clase de Ig conocidas. Se trabajó con 14 extractos de A. lumbricoides. Se realizó la prueba de Inhibición de la Aglutinación en medio enzimático de bromelina y temperatura de 4 ºC. Se determinó el título, Score y Parámetro de Sensibilidad de cada anticuerpo monoclonal frente a la suspensión de glóbulos rojos usada como sistema revelador. Diez extractos inhibieron la aglutinación de todos los anticuerpos monoclonales anti P1 con la suspensión de glóbulos rojos P1. Los 4 extractos restantes sólo inhibieron la aglutinación de algunos de ellos. Se demuestra que todos los extractos estudiados tienen actividad P1. Esta actividad es independiente del título, Score, Parámentro de Sensibilidad, concentración y clase de Ig del anticuerpo monoclonal y depende del epitope al cual esta dirigido el anticuerpo. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30672Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 1 (2003); 53-54 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 1 (2003); 53-54 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 1 (2003); 53-54 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30672/32556Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessPonce De León, PatriciaValverde, Juana2012-07-07T18:00:35Zoai:revistas.usp.br:article/30672Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:26.594836Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv P System antigenic determiners expression in Ascaris lumbricoides
Presencia de determinantes antigénicas P1 en Ascaris lumbricoides
title P System antigenic determiners expression in Ascaris lumbricoides
spellingShingle P System antigenic determiners expression in Ascaris lumbricoides
Ponce De León, Patricia
Ascaris lumbricoides
P System
title_short P System antigenic determiners expression in Ascaris lumbricoides
title_full P System antigenic determiners expression in Ascaris lumbricoides
title_fullStr P System antigenic determiners expression in Ascaris lumbricoides
title_full_unstemmed P System antigenic determiners expression in Ascaris lumbricoides
title_sort P System antigenic determiners expression in Ascaris lumbricoides
author Ponce De León, Patricia
author_facet Ponce De León, Patricia
Valverde, Juana
author_role author
author2 Valverde, Juana
author2_role author
dc.contributor.author.fl_str_mv Ponce De León, Patricia
Valverde, Juana
dc.subject.por.fl_str_mv Ascaris lumbricoides
P System
topic Ascaris lumbricoides
P System
description The P System antigens have been detected in numerous parasites, bacterias and viruses, nevertheless the clinical significance is still unknown. The aim was to study the presence of P1 antigenic determiners in A. lumbricoides extracts by means of the use of 6 different monoclonal antibodies of well-known concentrations and Ig class. We worked with 14 A. lumbricoides extracts. Inhibition Agglutination Test was made in a bromelin enzymatic medium and 4 ºC temperature. Titre, Score and Sensitivity Parameter were determined for each monoclonal antibody against red cells suspension used as revealing system. Ten extracts inhibited the agglutination of all anti P1 monoclonal antibodies. The 4 remaining extracts only inhibited the agglutination of some of them. It is demonstrated that the extracts have P1 activity. This activity is independent of titre, Score, Sensitivity Parameter, concentration and Ig class and it depends on the epitope at which the monoclonal antibody is directed.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30672
url https://www.revistas.usp.br/rimtsp/article/view/30672
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30672/32556
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 1 (2003); 53-54
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 1 (2003); 53-54
Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 1 (2003); 53-54
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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