Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay
Autor(a) principal: | |
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Data de Publicação: | 1990 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | spa |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28745 |
Resumo: | An enzymoimmunoassay (EIE) as a screening test to select potential asymptomatic cyst carriers among the general population of areas under risk is being used in programs for the control of hydatic diseases caused by Echinococcus granulosus in Argentina. The experience obtained up to date, applying this assay in population surveys, indicates that depending on the prevalence in the area 10% to 30% of the individuals selected did not show images compatible with hydatic cysts. The purpose of the present study was to improve the specificity of the test. To this purpose, the influence of the modification of the antigenic availability and the effect of the absorption from the serum samples of antibodies anti-normal ovine sera and anti-phosphorylcholine was evaluated. One hundred and fourteen non hydatic sera selected because of their high cross reactivity in EIE using the whole hydatid antigen (WHA) and 118 hydatid sera, were studied with four fractions of ovine hydatid cyst fluid. The EIE employing the S2B antigenic fraction with previous absorption of the sera (EIE-S2B/A) was the system that discriminated better hydatid sera from non hydatid sera with high levels of cross reactivity. The replacement of the EIE employing WHA by the EIE-S2B/A system, for the active search of asymptomatic cyst carriers in field conditions, is proposed. The four antigenic fractions were analyzed by double diffusion and SDS-PAGE. The S2B fraction revealed a high content of parasitic components of less than 30 Kd which probably includes antigen B and subunits or fragments of antigen 5. |
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Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay Detección de portadores asintomáticos de quistes hidatídicos: aumento de la especificidad del ensayo inmunoenzimático HidatidosisE. granulosusSeroepidemiologiaInmunodiagnóstico An enzymoimmunoassay (EIE) as a screening test to select potential asymptomatic cyst carriers among the general population of areas under risk is being used in programs for the control of hydatic diseases caused by Echinococcus granulosus in Argentina. The experience obtained up to date, applying this assay in population surveys, indicates that depending on the prevalence in the area 10% to 30% of the individuals selected did not show images compatible with hydatic cysts. The purpose of the present study was to improve the specificity of the test. To this purpose, the influence of the modification of the antigenic availability and the effect of the absorption from the serum samples of antibodies anti-normal ovine sera and anti-phosphorylcholine was evaluated. One hundred and fourteen non hydatic sera selected because of their high cross reactivity in EIE using the whole hydatid antigen (WHA) and 118 hydatid sera, were studied with four fractions of ovine hydatid cyst fluid. The EIE employing the S2B antigenic fraction with previous absorption of the sera (EIE-S2B/A) was the system that discriminated better hydatid sera from non hydatid sera with high levels of cross reactivity. The replacement of the EIE employing WHA by the EIE-S2B/A system, for the active search of asymptomatic cyst carriers in field conditions, is proposed. The four antigenic fractions were analyzed by double diffusion and SDS-PAGE. The S2B fraction revealed a high content of parasitic components of less than 30 Kd which probably includes antigen B and subunits or fragments of antigen 5. Una de las actividades que realizan los programas de control de hidatidosis causada por Echinococcus granulosus en la República Argentina es la búsqueda de portadores asintomáticos de quistes hidatídicos entre la población general que habita las áreas de riesgo, mediante un ensayo inmunoenzimático (EIE) con antígeno de liquido hidatídico total (EIE-ALHT) que selecciona los posibles portadores. En base a la experiencia recogida se ha observado que dependiendo de la prevalencia del área, entre el 10% y el 30% de las personas seleccionadas por el EIE con valores de densidad óptica (DO >; DO + 4S no presentan imagenes compatibles con quistes hidatídicos. El propósito del estudio fue mejorar la especificidad de la prueba. Con ese fin, se evaluó la influencia de la modificación de la oferta antigénica y el efecto de la absorción de los anticuerpos anti-componentes séricos ovinos y anti-fosforilcolina de los sueros en estudios. 114 sueros no hidatídicos seleccionados por su alto nivel de reacciones cruzadas en EIE-ALHT y 118 sueros hidatídicos se estudiaron frente a 4 fracciones antigénicas de liquido hidatídico ovino. El EIE que empleo la fracción antigénica S2B conjuntamente con la absorción previa de los sueros (EIE-S2B/A) fue el sistema que mejor discriminó los sueros hidatídicos de los no hidatídicos con altos niveles de anticuerpos no relacionados con la presencia de quistes. Se propone el empleo del sistema EIE-S2B/A para la búsqueda a campo de portadores asintomáticos de quistes hidatídicos en reemplazo del EIE-ALHT actualmente en uso. Las 4 fracciones antigénicas fueron analizadas por doble difusión y SDS-PAGE. La fracción S2B se caracterizó como enriquecida en componentes parasitarios de menos de 30 Kd entre los cuales se encontrarian el antígeno B y subunidades o fragmentos del antígeno 5. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1990-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28745Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 4 (1990); 275-284 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 4 (1990); 275-284 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 4 (1990); 275-284 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTspahttps://www.revistas.usp.br/rimtsp/article/view/28745/30598Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessColtorti, E. A.Fernández, E.Marguet, E. R.Scozzina, J. D.Guarnera, E. A.2012-07-02T01:28:17Zoai:revistas.usp.br:article/28745Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:29.959168Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay Detección de portadores asintomáticos de quistes hidatídicos: aumento de la especificidad del ensayo inmunoenzimático |
title |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay |
spellingShingle |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay Coltorti, E. A. Hidatidosis E. granulosus Seroepidemiologia Inmunodiagnóstico |
title_short |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay |
title_full |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay |
title_fullStr |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay |
title_full_unstemmed |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay |
title_sort |
Detection of asymptomatic hydatic cysts carriers by an enzymatic immunoassay |
author |
Coltorti, E. A. |
author_facet |
Coltorti, E. A. Fernández, E. Marguet, E. R. Scozzina, J. D. Guarnera, E. A. |
author_role |
author |
author2 |
Fernández, E. Marguet, E. R. Scozzina, J. D. Guarnera, E. A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Coltorti, E. A. Fernández, E. Marguet, E. R. Scozzina, J. D. Guarnera, E. A. |
dc.subject.por.fl_str_mv |
Hidatidosis E. granulosus Seroepidemiologia Inmunodiagnóstico |
topic |
Hidatidosis E. granulosus Seroepidemiologia Inmunodiagnóstico |
description |
An enzymoimmunoassay (EIE) as a screening test to select potential asymptomatic cyst carriers among the general population of areas under risk is being used in programs for the control of hydatic diseases caused by Echinococcus granulosus in Argentina. The experience obtained up to date, applying this assay in population surveys, indicates that depending on the prevalence in the area 10% to 30% of the individuals selected did not show images compatible with hydatic cysts. The purpose of the present study was to improve the specificity of the test. To this purpose, the influence of the modification of the antigenic availability and the effect of the absorption from the serum samples of antibodies anti-normal ovine sera and anti-phosphorylcholine was evaluated. One hundred and fourteen non hydatic sera selected because of their high cross reactivity in EIE using the whole hydatid antigen (WHA) and 118 hydatid sera, were studied with four fractions of ovine hydatid cyst fluid. The EIE employing the S2B antigenic fraction with previous absorption of the sera (EIE-S2B/A) was the system that discriminated better hydatid sera from non hydatid sera with high levels of cross reactivity. The replacement of the EIE employing WHA by the EIE-S2B/A system, for the active search of asymptomatic cyst carriers in field conditions, is proposed. The four antigenic fractions were analyzed by double diffusion and SDS-PAGE. The S2B fraction revealed a high content of parasitic components of less than 30 Kd which probably includes antigen B and subunits or fragments of antigen 5. |
publishDate |
1990 |
dc.date.none.fl_str_mv |
1990-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28745 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28745 |
dc.language.iso.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28745/30598 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 4 (1990); 275-284 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 4 (1990); 275-284 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 4 (1990); 275-284 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
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