Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30557 |
Resumo: | Eighty-one cerebrospinal fluid (CSF) samples mainly from cases of aseptic meningitis and motor deficiency syndrome were sent to the Virology Section of Evandro Chagas Institute, Belém Pará, in the period of January 1995 to January 1996 in order to isolate viruses. All samples were inoculated onto HEp-2 cell culture and newborn mice, with negative results. The probability of isolating viruses by these methods is reduced because of the low concentration of viral particles in these specimens. In order to obtain more information about the etiology of these cases, a group of 23 samples were selected to be tested by a more sensitive technique than the virus isolation - the reverse transcription polymerase chain reaction (RT-PCR). Specific primers directed to conserved regions in the enterovirus genome were used, considering that this group of viruses is frequently associated with these neurological disorder. The age of the patients ranged from 1 to 55 years and nearly all of them lived in Belém, State of Pará, North of Brazil. Of 15 samples analyzed by RT PCR nine (60%) were positive; of these, 6 (66.6%) had motor deficiency and 3 (33.3%) developed aseptic meningitis. These results show that it is important to investigate enterovirus as cause of these syndromes. |
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Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil Pesquisa de enterovírus em casos de meningite assética e síndrome de deficiência motora em Belém, Pará, Brasil Aseptic meningitisGBSEnteroviruses Eighty-one cerebrospinal fluid (CSF) samples mainly from cases of aseptic meningitis and motor deficiency syndrome were sent to the Virology Section of Evandro Chagas Institute, Belém Pará, in the period of January 1995 to January 1996 in order to isolate viruses. All samples were inoculated onto HEp-2 cell culture and newborn mice, with negative results. The probability of isolating viruses by these methods is reduced because of the low concentration of viral particles in these specimens. In order to obtain more information about the etiology of these cases, a group of 23 samples were selected to be tested by a more sensitive technique than the virus isolation - the reverse transcription polymerase chain reaction (RT-PCR). Specific primers directed to conserved regions in the enterovirus genome were used, considering that this group of viruses is frequently associated with these neurological disorder. The age of the patients ranged from 1 to 55 years and nearly all of them lived in Belém, State of Pará, North of Brazil. Of 15 samples analyzed by RT PCR nine (60%) were positive; of these, 6 (66.6%) had motor deficiency and 3 (33.3%) developed aseptic meningitis. These results show that it is important to investigate enterovirus as cause of these syndromes. Amostras de líquido céfalo raquidiano colhidas de 81casos (principalmente meningite asséptica e deficiência motora) foram recebidas na Seção de Virologia do Instituto Evandro Chagas em Belém, Pará no período de janeiro de 1995 a janeiro de 1996, para investigação etiológica. Todas as amostras foram inoculadas em células HEp-2 e em camundongos recém nascidos, com resultados negativos. A probabilidade de se isolar vírus por esse método é reduzida devido à baixa concentração de partículas virais nesses espécimes. Com o objetivo de se obter mais informações sobre a etiologia desses casos, um grupo de 15 amostras, foram testadas usando a reação em cadeia de polimerase com transcrição reversa uma técnica mais sensível que o isolamento. Foram usados "iniciadores" específicos direcionados à região de seqüências conservadas dos enterovírus, considerando que este grupo de vírus é mais freqüentemente relacionado a desordens neurológicas. A idade dos pacientes variou de 1 a 55 anos, e quase todos viviam em Belém, Pará, norte do Brasil. Das 15 amostras analisadas, nove (60%) foram positivas - 6 (66,6%) relacionadas a deficiência motora e 3 (33,3%) a meningite asséptica. Esses resultados, indicam a importância de investigar enterovírus como etiologia dessas síndromes. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30557Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 6 (2001); 321-324 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 6 (2001); 321-324 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 6 (2001); 321-324 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30557/32441Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGOMES, Maria de Lourdes ContenteKOPECKA, HelenaLINHARES, Alexandre da Costa2012-07-07T13:36:09Zoai:revistas.usp.br:article/30557Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:20.535156Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil Pesquisa de enterovírus em casos de meningite assética e síndrome de deficiência motora em Belém, Pará, Brasil |
title |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil |
spellingShingle |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil GOMES, Maria de Lourdes Contente Aseptic meningitis GBS Enteroviruses |
title_short |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil |
title_full |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil |
title_fullStr |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil |
title_full_unstemmed |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil |
title_sort |
Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil |
author |
GOMES, Maria de Lourdes Contente |
author_facet |
GOMES, Maria de Lourdes Contente KOPECKA, Helena LINHARES, Alexandre da Costa |
author_role |
author |
author2 |
KOPECKA, Helena LINHARES, Alexandre da Costa |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
GOMES, Maria de Lourdes Contente KOPECKA, Helena LINHARES, Alexandre da Costa |
dc.subject.por.fl_str_mv |
Aseptic meningitis GBS Enteroviruses |
topic |
Aseptic meningitis GBS Enteroviruses |
description |
Eighty-one cerebrospinal fluid (CSF) samples mainly from cases of aseptic meningitis and motor deficiency syndrome were sent to the Virology Section of Evandro Chagas Institute, Belém Pará, in the period of January 1995 to January 1996 in order to isolate viruses. All samples were inoculated onto HEp-2 cell culture and newborn mice, with negative results. The probability of isolating viruses by these methods is reduced because of the low concentration of viral particles in these specimens. In order to obtain more information about the etiology of these cases, a group of 23 samples were selected to be tested by a more sensitive technique than the virus isolation - the reverse transcription polymerase chain reaction (RT-PCR). Specific primers directed to conserved regions in the enterovirus genome were used, considering that this group of viruses is frequently associated with these neurological disorder. The age of the patients ranged from 1 to 55 years and nearly all of them lived in Belém, State of Pará, North of Brazil. Of 15 samples analyzed by RT PCR nine (60%) were positive; of these, 6 (66.6%) had motor deficiency and 3 (33.3%) developed aseptic meningitis. These results show that it is important to investigate enterovirus as cause of these syndromes. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30557 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30557 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30557/32441 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 6 (2001); 321-324 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 6 (2001); 321-324 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 6 (2001); 321-324 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951643348205568 |