Accidental infection by Trypanosoma cruzi follow-up by the polymerase chain reaction: case report

• Main Author: Kinoshita-Yanaga, Andréa Tieko
• Publication Date: 2009
• Other Authors: Toledo, Max Jean de Ornelas, Araújo, Silvana Marques de, Vier, Berenice Pelizza, Gomes, Mônica Lúcia
• Format: Article
• Language: eng
• Source: Revista do Instituto de Medicina Tropical de São Paulo
• Download full: https://www.revistas.usp.br/rimtsp/article/view/31292
• topic: Trypanosoma cruzi; Laboratory accident; PCR; Diagnosis; Follow-up

We report a case of accidental infection by Trypanosoma cruzi in a 42-year-old female patient who presented an inoculation chagoma. Laboratory confirmation was based on examination of fresh blood, Giemsa-stained blood smear, immunoenzyme test (EIA-IgG), indirect immunofluorescence (IIF-IgM, IgG) and polymerase chain reaction (PCR). Only the PCR gave a positive result, and the EIA test was inconclusive. Two treatments with benznidazole were necessary. PCR was the only technique that continued to give positive results for approximately two months (65 days, or 2.2 months) following the second treatment and negative results from 96 days (3.2 months) to 850 days (28.3 months). We concluded that the presence of an inoculation chagoma and use of PCR were important and decisive for diagnosis and follow-up of the case.