Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Krzyzanowsky, Flávio
Data de Publicação: 2003
Outros Autores: Avila-Campos, Mario J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30724
Resumo: Non-enterotoxigenic bacteria of the Bacteroides fragilis group and enterotoxigenic B. fragilis were identified from children with and without aqueous acute diarrhea. In this study, 170 stool samples from 96 children with and 74 without diarrhea were analyzed. Enterotoxin production and the toxin gene detection were detected by cytotoxicity assay on HT-29/C1 cells and by PCR, respectively. B. fragilis species was prevalent in both groups and enterotoxigenic B. fragilis strains were isolated from two children with diarrhea. More studies are important to evaluate the role of each bacteria of the B. fragilis group, including enterotoxigenic strains play in the diarrheal processes in children.
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spelling Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil Detecção de Bacteroides fragilis enterotoxigênicos e não enterotoxigênicos de amostras fecais de crianças em São Paulo, Brasil Bacteroides fragilis groupETBFAcute diarrheaChildren Non-enterotoxigenic bacteria of the Bacteroides fragilis group and enterotoxigenic B. fragilis were identified from children with and without aqueous acute diarrhea. In this study, 170 stool samples from 96 children with and 74 without diarrhea were analyzed. Enterotoxin production and the toxin gene detection were detected by cytotoxicity assay on HT-29/C1 cells and by PCR, respectively. B. fragilis species was prevalent in both groups and enterotoxigenic B. fragilis strains were isolated from two children with diarrhea. More studies are important to evaluate the role of each bacteria of the B. fragilis group, including enterotoxigenic strains play in the diarrheal processes in children. Bactérias não enterotoxigênicas do grupo Bacteroides fragilis e B. fragilis enterotoxigênicas foram isoladas e identificadas de crianças com e sem diarréia aguda aquosa. Neste estudo, 170 amostras fecais de 96 e 74 crianças, com e sem diarréia, respectivamente, foram analisadas. A produção de enterotoxina e a detecção do gene que media a produção da toxina foram determinadas por ensaios citotóxicos em células HT-29/C1 e por PCR, respectivamente. A espécie B. fragilis foi prevalente em ambos os grupos, e cepas de B. fragilis enterotoxigênicas foram isoladas de duas crianças com diarréia aquosa aguda. Maiores estudos são necessários para avaliar o papel de cada bactéria desse importante grupo bacteriano, incluindo-se o papel que as cepas enterotoxigênicas, desempenham no processo diarréico em crianças. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30724Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 4 (2003); 225-227 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 4 (2003); 225-227 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 4 (2003); 225-227 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30724/32608Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessKrzyzanowsky, FlávioAvila-Campos, Mario J.2012-07-07T18:03:05Zoai:revistas.usp.br:article/30724Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:29.251027Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
Detecção de Bacteroides fragilis enterotoxigênicos e não enterotoxigênicos de amostras fecais de crianças em São Paulo, Brasil
title Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
spellingShingle Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
Krzyzanowsky, Flávio
Bacteroides fragilis group
ETBF
Acute diarrhea
Children
title_short Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
title_full Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
title_fullStr Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
title_full_unstemmed Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
title_sort Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
author Krzyzanowsky, Flávio
author_facet Krzyzanowsky, Flávio
Avila-Campos, Mario J.
author_role author
author2 Avila-Campos, Mario J.
author2_role author
dc.contributor.author.fl_str_mv Krzyzanowsky, Flávio
Avila-Campos, Mario J.
dc.subject.por.fl_str_mv Bacteroides fragilis group
ETBF
Acute diarrhea
Children
topic Bacteroides fragilis group
ETBF
Acute diarrhea
Children
description Non-enterotoxigenic bacteria of the Bacteroides fragilis group and enterotoxigenic B. fragilis were identified from children with and without aqueous acute diarrhea. In this study, 170 stool samples from 96 children with and 74 without diarrhea were analyzed. Enterotoxin production and the toxin gene detection were detected by cytotoxicity assay on HT-29/C1 cells and by PCR, respectively. B. fragilis species was prevalent in both groups and enterotoxigenic B. fragilis strains were isolated from two children with diarrhea. More studies are important to evaluate the role of each bacteria of the B. fragilis group, including enterotoxigenic strains play in the diarrheal processes in children.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30724
url https://www.revistas.usp.br/rimtsp/article/view/30724
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30724/32608
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 4 (2003); 225-227
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 4 (2003); 225-227
Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 4 (2003); 225-227
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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