Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/166315 |
Resumo: | In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female (p = 0.03), having diabetes (p < 0.01), history of previous TB disease (p < 0.01), and the number of intra-domiciliary contacts (p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%), rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza. |
id |
IMT-1_f30c4756b3d9a1fc08727d82e209a28c |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/166315 |
network_acronym_str |
IMT-1 |
network_name_str |
Revista do Instituto de Medicina Tropical de São Paulo |
repository_id_str |
|
spelling |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast BrazilMycobacterium tuberculosisMultidrug-resistant TBPrimary resistancePre-extensively drug-resistant TBRisk factorsIn Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female (p = 0.03), having diabetes (p < 0.01), history of previous TB disease (p < 0.01), and the number of intra-domiciliary contacts (p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%), rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2020-02-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/16631510.1590/S1678-9946202062004Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e41678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/166315/159043https://www.revistas.usp.br/rimtsp/article/view/166315/159044Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCampelo, Thales AlvesLima, Luana Nepomuceno CostaLima, Karla Valéria BatistaSilva, Caroliny SoaresConceição, Marília Lima daBarreto, José Antonio PereiraMota, Aquiles Paulino PeresSancho, Soraya de OliveiraFrota, Cristiane Cunha2020-10-26T17:39:25Zoai:revistas.usp.br:article/166315Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:51.325049Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
title |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
spellingShingle |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil Campelo, Thales Alves Mycobacterium tuberculosis Multidrug-resistant TB Primary resistance Pre-extensively drug-resistant TB Risk factors |
title_short |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
title_full |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
title_fullStr |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
title_full_unstemmed |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
title_sort |
Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil |
author |
Campelo, Thales Alves |
author_facet |
Campelo, Thales Alves Lima, Luana Nepomuceno Costa Lima, Karla Valéria Batista Silva, Caroliny Soares Conceição, Marília Lima da Barreto, José Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha |
author_role |
author |
author2 |
Lima, Luana Nepomuceno Costa Lima, Karla Valéria Batista Silva, Caroliny Soares Conceição, Marília Lima da Barreto, José Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Campelo, Thales Alves Lima, Luana Nepomuceno Costa Lima, Karla Valéria Batista Silva, Caroliny Soares Conceição, Marília Lima da Barreto, José Antonio Pereira Mota, Aquiles Paulino Peres Sancho, Soraya de Oliveira Frota, Cristiane Cunha |
dc.subject.por.fl_str_mv |
Mycobacterium tuberculosis Multidrug-resistant TB Primary resistance Pre-extensively drug-resistant TB Risk factors |
topic |
Mycobacterium tuberculosis Multidrug-resistant TB Primary resistance Pre-extensively drug-resistant TB Risk factors |
description |
In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female (p = 0.03), having diabetes (p < 0.01), history of previous TB disease (p < 0.01), and the number of intra-domiciliary contacts (p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%), rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/166315 10.1590/S1678-9946202062004 |
url |
https://www.revistas.usp.br/rimtsp/article/view/166315 |
identifier_str_mv |
10.1590/S1678-9946202062004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/166315/159043 https://www.revistas.usp.br/rimtsp/article/view/166315/159044 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4 Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e4 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951652653268992 |