Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil

Detalhes bibliográficos
Autor(a) principal: Campelo, Thales Alves
Data de Publicação: 2020
Outros Autores: Lima, Luana Nepomuceno Costa, Lima, Karla Valéria Batista, Silva, Caroliny Soares, Conceição, Marília Lima da, Barreto, José Antonio Pereira, Mota, Aquiles Paulino Peres, Sancho, Soraya de Oliveira, Frota, Cristiane Cunha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/166315
Resumo: In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female (p = 0.03), having diabetes (p < 0.01), history of previous TB disease (p < 0.01), and the number of intra-domiciliary contacts (p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%), rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza.
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spelling Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast BrazilMycobacterium tuberculosisMultidrug-resistant TBPrimary resistancePre-extensively drug-resistant TBRisk factorsIn Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female (p = 0.03), having diabetes (p < 0.01), history of previous TB disease (p < 0.01), and the number of intra-domiciliary contacts (p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%), rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2020-02-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/16631510.1590/S1678-9946202062004Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e41678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/166315/159043https://www.revistas.usp.br/rimtsp/article/view/166315/159044Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCampelo, Thales AlvesLima, Luana Nepomuceno CostaLima, Karla Valéria BatistaSilva, Caroliny SoaresConceição, Marília Lima daBarreto, José Antonio PereiraMota, Aquiles Paulino PeresSancho, Soraya de OliveiraFrota, Cristiane Cunha2020-10-26T17:39:25Zoai:revistas.usp.br:article/166315Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:51.325049Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
title Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
spellingShingle Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
Campelo, Thales Alves
Mycobacterium tuberculosis
Multidrug-resistant TB
Primary resistance
Pre-extensively drug-resistant TB
Risk factors
title_short Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
title_full Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
title_fullStr Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
title_full_unstemmed Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
title_sort Molecular characterization of pre-extensive drug resistant Mycobacterium tuberculosis in Northeast Brazil
author Campelo, Thales Alves
author_facet Campelo, Thales Alves
Lima, Luana Nepomuceno Costa
Lima, Karla Valéria Batista
Silva, Caroliny Soares
Conceição, Marília Lima da
Barreto, José Antonio Pereira
Mota, Aquiles Paulino Peres
Sancho, Soraya de Oliveira
Frota, Cristiane Cunha
author_role author
author2 Lima, Luana Nepomuceno Costa
Lima, Karla Valéria Batista
Silva, Caroliny Soares
Conceição, Marília Lima da
Barreto, José Antonio Pereira
Mota, Aquiles Paulino Peres
Sancho, Soraya de Oliveira
Frota, Cristiane Cunha
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campelo, Thales Alves
Lima, Luana Nepomuceno Costa
Lima, Karla Valéria Batista
Silva, Caroliny Soares
Conceição, Marília Lima da
Barreto, José Antonio Pereira
Mota, Aquiles Paulino Peres
Sancho, Soraya de Oliveira
Frota, Cristiane Cunha
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
Multidrug-resistant TB
Primary resistance
Pre-extensively drug-resistant TB
Risk factors
topic Mycobacterium tuberculosis
Multidrug-resistant TB
Primary resistance
Pre-extensively drug-resistant TB
Risk factors
description In Fortaleza, the capital of Ceara State, Brazil, the detection rate of tuberculosis (TB) in 2018 was 65.5/100,000 inhabitants with a cure rate of 59.1%, which is higher than the country average. This study investigated the risk factors associated with drug-resistant tuberculosis (DR-TB) and identified the drug-resistance phenotype and resistance-conferring mutations. The geographic distribution of DR-TB in Fortaleza, Brazil, was also determined. From March 2017 to February 2018, 41 DR-TB isolates and 69 drug-susceptible pulmonary TB isolates were obtained from patients seen at a referral hospital in Fortaleza, Brazil. Samples were subjected to phenotypic and genetic analysis of resistance; the spatial distribution of the participants was also analyzed. Primary resistance was high (50.9%) among participants. The following risk factors for DR were identified: being female (p = 0.03), having diabetes (p < 0.01), history of previous TB disease (p < 0.01), and the number of intra-domiciliary contacts (p < 0.01). Analysis by multiplex allele-specific polymerase chain reaction detected mutations in the genes katG (65.8%), rpoB (43.9%), inhA promoter (14.6%), and gyrA (9.8%). Sequencing identified mutations in the the genes katG (75.6%), inhA promoter (19.5%), rpoB (85.4%), and gyrA (100%). There was no mutation in the rrs gene. Spatial analysis showed DR-TB isolates distributed in areas of low socioeconomic status in the city of Fortaleza. Our results emphasized the importance of detecting resistance to TB drugs. The resistance found in the gene gyrA is of concern due to the high number of pre-extensive DR-TB cases in Fortaleza.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/166315
10.1590/S1678-9946202062004
url https://www.revistas.usp.br/rimtsp/article/view/166315
identifier_str_mv 10.1590/S1678-9946202062004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/166315/159043
https://www.revistas.usp.br/rimtsp/article/view/166315/159044
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e4
Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e4
1678-9946
0036-4665
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