Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia

Detalhes bibliográficos
Autor(a) principal: Martins Filho, Eduardo dos Santos
Data de Publicação: 2021
Outros Autores: Brito Junior, Lacy Cardoso de, Azevedo, Murilo Chermont, Paixão, Ana Paula Silveira, Carneiro, Debora Monteiro, Nascimento , Matheus Holanda
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Revista Brasileira de Cancerologia (Online)
DOI: 10.32635/2176-9745.RBC.2021v67n3.1228
Texto Completo: https://rbc.inca.gov.br/index.php/revista/article/view/1228
Resumo: Introduction: The potential for malignant transformation of hematopoietic stem cells carrying mutations in the glycosylphosphatidylinositol class A (PIG-A) gene for acute leukemias, although rare, is already well described in the literature. Objective: In this study, however, it was attempted to show for the first time in the literature the emergence or maintenance of paroxysmal nocturnal hemoglobinuria (PNH) clones in patients diagnosed with acute leukemia or even after the beginning of the chemotherapy treatment. Method: The search of PNH clones was performed by flow cytometry in blasts, erythrocytes, granulocytes or monocytes of 47 samples of peripheral blood and bone marrow from patients undergoing diagnostic investigation or therapeutic follow-up in two oncological and public hospitals in Belém, from December 2017 to December 2018. Results: The presence of PNH clones was observed in 19/47 (40.4%) patient samples, in diagnostic investigation or therapeutic follow-up, who participated of at least one therapeutic follow-up study and still experience the appearance or maintenance of the PNH clone even after the beginning of the chemotherapy treatment. Conclusion: Primarily, it was possible to demonstrate the presence of PNH clones in patients diagnosed with acute leukemia both during the diagnostic investigation period and therapeutic follow-up, regardless of cell ontogeny. However, the importance of the presence of these PNH clones for the evolution of the primary disease, prognosis or need for specific treatment was not evaluated yet.
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spelling Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute LeukemiaEstudio Pionero sobre la Presencia de un Clon de Hemoglobinuria Paroxística Nocturna durante el Seguimiento Terapéutico de la Leucemia AgudaEstudo Pioneiro sobre a Presença de Clone de Hemoglobinúria Paroxística Noturna durante Acompanhamento Terapêutico de Leucemia AgudaHemoglobinúria Paroxística/diagnósticoHemoglobinúria Paroxística/tratamento farmacológicoLeucemia/diagnósticoDoença AgudaHemoglobinuria, Paroxysmal/diagnosisHemoglobinuria, Paroxysmal/drug therapyLeukemia/diagnosisAcute DiseaseHemoglobinuria Paroxística/diagnósticoHemoglobinuria Paroxística/tratamiento farmacológicoLeucemia/diagnósticoEnfermedad AgudaIntroduction: The potential for malignant transformation of hematopoietic stem cells carrying mutations in the glycosylphosphatidylinositol class A (PIG-A) gene for acute leukemias, although rare, is already well described in the literature. Objective: In this study, however, it was attempted to show for the first time in the literature the emergence or maintenance of paroxysmal nocturnal hemoglobinuria (PNH) clones in patients diagnosed with acute leukemia or even after the beginning of the chemotherapy treatment. Method: The search of PNH clones was performed by flow cytometry in blasts, erythrocytes, granulocytes or monocytes of 47 samples of peripheral blood and bone marrow from patients undergoing diagnostic investigation or therapeutic follow-up in two oncological and public hospitals in Belém, from December 2017 to December 2018. Results: The presence of PNH clones was observed in 19/47 (40.4%) patient samples, in diagnostic investigation or therapeutic follow-up, who participated of at least one therapeutic follow-up study and still experience the appearance or maintenance of the PNH clone even after the beginning of the chemotherapy treatment. Conclusion: Primarily, it was possible to demonstrate the presence of PNH clones in patients diagnosed with acute leukemia both during the diagnostic investigation period and therapeutic follow-up, regardless of cell ontogeny. However, the importance of the presence of these PNH clones for the evolution of the primary disease, prognosis or need for specific treatment was not evaluated yet.Introducción: El potencial de transformación maligna de las células madre hematopoyéticas que portan mutaciones en el gen glicosofosfatidilinositol (GPI) clase A (PIGA) para las leucemias agudas, aunque raro, ya está bien descrito en la literatura. Objetivo: En este estudio, sin embargo, buscamos mostrar por primera vez en la literatura la aparición o mantenimiento de clones de HPN en pacientes diagnosticados de leucemia aguda o incluso después del inicio de la quimioterapia. Método: La investigación de clones de hemoglobinuria paroxística nocturna (HPN) se realizó mediante citometría de flujo en blastos, eritrocitos, granulocitos o monocitos de 47 muestras de sangre periférica y médula ósea de pacientes sometidos a investigación diagnóstica o seguimiento terapéutico de dos hospitales oncológicos y públicos de Belém, durante el período. de diciembre de 2017 a diciembre de 2018. Resultados: La presencia de clones HPN se observó en 19/47 (40,4%) muestras de pacientes, en investigación diagnóstica o seguimiento terapéutico, que realizaron al menos un estudio de seguimiento terapéutico y aún tenían la aparición o mantenimiento del clon HPN incluso después de iniciado el tratamiento de quimioterapia. Conclusión: Se pudo evidenciar, de forma primaria, la presencia de clones de HPN en pacientes diagnosticados de leucemia aguda tanto durante el período de investigación diagnóstica como durante el seguimiento terapéutico, independientemente de la ontogenia celular. Sin embargo, no podemos todavía evaluar la importancia de la presencia de estos clones de HPN para la evolución de la enfermedad primaria, el pronóstico o la necesidad de un tratamiento específico.Introdução: O potencial de transformação maligna de células-tronco hematopoiéticas portadoras de mutações no gene glicosilfostatidilinositol classe A (PIG-A) para leucemias agudas, embora raro, já é bem descrito na literatura. Objetivo: Neste estudo, porém, buscou-se evidenciar pela primeira vez na literatura o surgimento ou a manutenção de clones de hemoglobinúria paroxística noturna (HPN) em pacientes diagnosticados com leucemia aguda ou ainda após o início do tratamento quimioterápico. Método: A pesquisa de clones de HPN foi realizada por citometria de fluxo em blastos, hemácias, granulócitos ou monócitos de 47 amostras de sangue periférico e medula óssea de pacientes submetidos à investigação diagnóstica ou acompanhamento terapêutico, provenientes de dois hospitais oncológicos e públicos de Belém, no período de dezembro de 2017 a dezembro de 2018. Resultados: A presença de clones de HPN foi observada em 19/47 (40,4%) amostras de pacientes, em investigação diagnóstica ou acompanhamento terapêutico, que realizaram pelo menos um estudo de acompanhamento terapêutico e ainda tiveram o surgimento ou a manutenção do clone de HPN mesmo após iniciado o tratamento quimioterápico. Conclusão: Foi possível evidenciar, de forma primária, a presença de clones de HPN em pacientes diagnosticados com leucemia aguda tanto no período de investigação diagnóstica como durante o acompanhamento terapêutico, independentemente da ontogenia celular. Sem, porém, que se possa ainda avaliar a importância da presença desses clones de HPN para a evolução da doença primária, prognóstico ou necessidade de tratamento específico.INCA2021-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigos, Avaliado pelos paresapplication/pdfapplication/pdfhttps://rbc.inca.gov.br/index.php/revista/article/view/122810.32635/2176-9745.RBC.2021v67n3.1228Revista Brasileira de Cancerologia; Vol. 67 No. 3 (2021): July/Aug./Sept.; e-091228Revista Brasileira de Cancerologia; Vol. 67 Núm. 3 (2021): jul./ago./sept.; e-091228Revista Brasileira de Cancerologia; v. 67 n. 3 (2021): jul./ago./set.; e-0912282176-9745reponame:Revista Brasileira de Cancerologia (Online)instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)instacron:INCAporenghttps://rbc.inca.gov.br/index.php/revista/article/view/1228/1324https://rbc.inca.gov.br/index.php/revista/article/view/1228/1469Copyright (c) 2021 Revista Brasileira de Cancerologiainfo:eu-repo/semantics/openAccessMartins Filho, Eduardo dos Santos Brito Junior, Lacy Cardoso de Azevedo, Murilo Chermont Paixão, Ana Paula Silveira Carneiro, Debora Monteiro Nascimento , Matheus Holanda 2021-11-29T18:25:18Zoai:rbc.inca.gov.br:article/1228Revistahttps://rbc.inca.gov.br/index.php/revistaPUBhttps://rbc.inca.gov.br/index.php/revista/oairbc@inca.gov.br0034-71162176-9745opendoar:2021-11-29T18:25:18Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)false
dc.title.none.fl_str_mv Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
Estudio Pionero sobre la Presencia de un Clon de Hemoglobinuria Paroxística Nocturna durante el Seguimiento Terapéutico de la Leucemia Aguda
Estudo Pioneiro sobre a Presença de Clone de Hemoglobinúria Paroxística Noturna durante Acompanhamento Terapêutico de Leucemia Aguda
title Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
spellingShingle Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
Martins Filho, Eduardo dos Santos
Hemoglobinúria Paroxística/diagnóstico
Hemoglobinúria Paroxística/tratamento farmacológico
Leucemia/diagnóstico
Doença Aguda
Hemoglobinuria, Paroxysmal/diagnosis
Hemoglobinuria, Paroxysmal/drug therapy
Leukemia/diagnosis
Acute Disease
Hemoglobinuria Paroxística/diagnóstico
Hemoglobinuria Paroxística/tratamiento farmacológico
Leucemia/diagnóstico
Enfermedad Aguda
Martins Filho, Eduardo dos Santos
Hemoglobinúria Paroxística/diagnóstico
Hemoglobinúria Paroxística/tratamento farmacológico
Leucemia/diagnóstico
Doença Aguda
Hemoglobinuria, Paroxysmal/diagnosis
Hemoglobinuria, Paroxysmal/drug therapy
Leukemia/diagnosis
Acute Disease
Hemoglobinuria Paroxística/diagnóstico
Hemoglobinuria Paroxística/tratamiento farmacológico
Leucemia/diagnóstico
Enfermedad Aguda
title_short Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
title_full Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
title_fullStr Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
title_full_unstemmed Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
title_sort Pioneering Study about the Presence of Paroxysmal Nocturnal Hemoglobinuria Clone during Therapeutic Monitoring of Acute Leukemia
author Martins Filho, Eduardo dos Santos
author_facet Martins Filho, Eduardo dos Santos
Martins Filho, Eduardo dos Santos
Brito Junior, Lacy Cardoso de
Azevedo, Murilo Chermont
Paixão, Ana Paula Silveira
Carneiro, Debora Monteiro
Nascimento , Matheus Holanda
Brito Junior, Lacy Cardoso de
Azevedo, Murilo Chermont
Paixão, Ana Paula Silveira
Carneiro, Debora Monteiro
Nascimento , Matheus Holanda
author_role author
author2 Brito Junior, Lacy Cardoso de
Azevedo, Murilo Chermont
Paixão, Ana Paula Silveira
Carneiro, Debora Monteiro
Nascimento , Matheus Holanda
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Martins Filho, Eduardo dos Santos
Brito Junior, Lacy Cardoso de
Azevedo, Murilo Chermont
Paixão, Ana Paula Silveira
Carneiro, Debora Monteiro
Nascimento , Matheus Holanda
dc.subject.por.fl_str_mv Hemoglobinúria Paroxística/diagnóstico
Hemoglobinúria Paroxística/tratamento farmacológico
Leucemia/diagnóstico
Doença Aguda
Hemoglobinuria, Paroxysmal/diagnosis
Hemoglobinuria, Paroxysmal/drug therapy
Leukemia/diagnosis
Acute Disease
Hemoglobinuria Paroxística/diagnóstico
Hemoglobinuria Paroxística/tratamiento farmacológico
Leucemia/diagnóstico
Enfermedad Aguda
topic Hemoglobinúria Paroxística/diagnóstico
Hemoglobinúria Paroxística/tratamento farmacológico
Leucemia/diagnóstico
Doença Aguda
Hemoglobinuria, Paroxysmal/diagnosis
Hemoglobinuria, Paroxysmal/drug therapy
Leukemia/diagnosis
Acute Disease
Hemoglobinuria Paroxística/diagnóstico
Hemoglobinuria Paroxística/tratamiento farmacológico
Leucemia/diagnóstico
Enfermedad Aguda
description Introduction: The potential for malignant transformation of hematopoietic stem cells carrying mutations in the glycosylphosphatidylinositol class A (PIG-A) gene for acute leukemias, although rare, is already well described in the literature. Objective: In this study, however, it was attempted to show for the first time in the literature the emergence or maintenance of paroxysmal nocturnal hemoglobinuria (PNH) clones in patients diagnosed with acute leukemia or even after the beginning of the chemotherapy treatment. Method: The search of PNH clones was performed by flow cytometry in blasts, erythrocytes, granulocytes or monocytes of 47 samples of peripheral blood and bone marrow from patients undergoing diagnostic investigation or therapeutic follow-up in two oncological and public hospitals in Belém, from December 2017 to December 2018. Results: The presence of PNH clones was observed in 19/47 (40.4%) patient samples, in diagnostic investigation or therapeutic follow-up, who participated of at least one therapeutic follow-up study and still experience the appearance or maintenance of the PNH clone even after the beginning of the chemotherapy treatment. Conclusion: Primarily, it was possible to demonstrate the presence of PNH clones in patients diagnosed with acute leukemia both during the diagnostic investigation period and therapeutic follow-up, regardless of cell ontogeny. However, the importance of the presence of these PNH clones for the evolution of the primary disease, prognosis or need for specific treatment was not evaluated yet.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigos, Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbc.inca.gov.br/index.php/revista/article/view/1228
10.32635/2176-9745.RBC.2021v67n3.1228
url https://rbc.inca.gov.br/index.php/revista/article/view/1228
identifier_str_mv 10.32635/2176-9745.RBC.2021v67n3.1228
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://rbc.inca.gov.br/index.php/revista/article/view/1228/1324
https://rbc.inca.gov.br/index.php/revista/article/view/1228/1469
dc.rights.driver.fl_str_mv Copyright (c) 2021 Revista Brasileira de Cancerologia
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Revista Brasileira de Cancerologia
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv INCA
publisher.none.fl_str_mv INCA
dc.source.none.fl_str_mv Revista Brasileira de Cancerologia; Vol. 67 No. 3 (2021): July/Aug./Sept.; e-091228
Revista Brasileira de Cancerologia; Vol. 67 Núm. 3 (2021): jul./ago./sept.; e-091228
Revista Brasileira de Cancerologia; v. 67 n. 3 (2021): jul./ago./set.; e-091228
2176-9745
reponame:Revista Brasileira de Cancerologia (Online)
instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
instacron:INCA
instname_str Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
instacron_str INCA
institution INCA
reponame_str Revista Brasileira de Cancerologia (Online)
collection Revista Brasileira de Cancerologia (Online)
repository.name.fl_str_mv Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
repository.mail.fl_str_mv rbc@inca.gov.br
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dc.identifier.doi.none.fl_str_mv 10.32635/2176-9745.RBC.2021v67n3.1228

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