Cell cycle: regulatory mechanisms and biochemical markers
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira de Cancerologia (Online) |
Texto Completo: | https://rbc.inca.gov.br/index.php/revista/article/view/2983 |
Resumo: | The regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability. |
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Cell cycle: regulatory mechanisms and biochemical markersCiclo celular: mecanismos reguladores e marcadores bioquímicosCiclo CelularCélula ProliferativaMarcadores Do Ciclo CelularProto-OncogenesCellular CycleProliferative CellCell-Cycle MarkersProto-OncogenesThe regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability.Os mecanismos reguladores do ciclo celular e sua inter-relação com fatores de crescimento, oncogenes e anti-oncogenes têm se constituído nas áreas de maior avanço no estudo da biologia do câncer. Continuamente estão sendo descobertas novas proteínas envolvidas no complexo mecanismo que desencadeia as diferentes fases do ciclo celular, como também os seus mecanismos de ativação. A descoberta dessas proteínas e co-fatores que regulam as transições G1/S e G2/M e que atuam na replicação do material bem como o papel dos proto-oncogenes dentro do ciclo celular permitiu aumentar o conhecimento sobre as células ciclizantes como tarnbém, permitiu que estas pudessem ser detectadas mesmo sem a visualização de figuras mitóticas. O desenvolvimento de anticorpos monoclonais contra estas proteínas tornou viável o estudo, em larga escala, de taxas de proliferação celular em diferentes neoplaslas, uma vez que substâncias como Ki67, polimerase alfa e PCNA - Ciclina somente são expressas em células em proliferação. A detecção de células proliferativas permite, através de sua contagem, estimar as taxas do crescimento neoplásico com conseqüente repercussão no prognóstico da doença e na terapêutica do paciente. Neste artigo são revistos os mecanismos de controle do ciclo celular bem como os métodos de detecção de células proliferativas, enfatizando a aplicação de marcadores pela sua praticidade e confiabilidade.INCA2023-06-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigos, Avaliado pelos paresapplication/pdfhttps://rbc.inca.gov.br/index.php/revista/article/view/298310.32635/2176-9745.RBC.1994v40n3.2983Revista Brasileira de Cancerologia; Vol. 40 No. 3 (1994): July/Aug./Sept.; 141-147Revista Brasileira de Cancerologia; Vol. 40 Núm. 3 (1994): jul./ago./sept.; 141-147Revista Brasileira de Cancerologia; v. 40 n. 3 (1994): jul./ago./set.; 141-1472176-9745reponame:Revista Brasileira de Cancerologia (Online)instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)instacron:INCAporhttps://rbc.inca.gov.br/index.php/revista/article/view/2983/1855https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRabenhorst, Silvia H.Burini, Roberto C. Schmitt, Fernando C. L. 2023-06-19T13:30:44Zoai:rbc.inca.gov.br:article/2983Revistahttps://rbc.inca.gov.br/index.php/revistaPUBhttps://rbc.inca.gov.br/index.php/revista/oairbc@inca.gov.br0034-71162176-9745opendoar:2023-06-19T13:30:44Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)false |
dc.title.none.fl_str_mv |
Cell cycle: regulatory mechanisms and biochemical markers Ciclo celular: mecanismos reguladores e marcadores bioquímicos |
title |
Cell cycle: regulatory mechanisms and biochemical markers |
spellingShingle |
Cell cycle: regulatory mechanisms and biochemical markers Rabenhorst, Silvia H. Ciclo Celular Célula Proliferativa Marcadores Do Ciclo Celular Proto-Oncogenes Cellular Cycle Proliferative Cell Cell-Cycle Markers Proto-Oncogenes |
title_short |
Cell cycle: regulatory mechanisms and biochemical markers |
title_full |
Cell cycle: regulatory mechanisms and biochemical markers |
title_fullStr |
Cell cycle: regulatory mechanisms and biochemical markers |
title_full_unstemmed |
Cell cycle: regulatory mechanisms and biochemical markers |
title_sort |
Cell cycle: regulatory mechanisms and biochemical markers |
author |
Rabenhorst, Silvia H. |
author_facet |
Rabenhorst, Silvia H. Burini, Roberto C. Schmitt, Fernando C. L. |
author_role |
author |
author2 |
Burini, Roberto C. Schmitt, Fernando C. L. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rabenhorst, Silvia H. Burini, Roberto C. Schmitt, Fernando C. L. |
dc.subject.por.fl_str_mv |
Ciclo Celular Célula Proliferativa Marcadores Do Ciclo Celular Proto-Oncogenes Cellular Cycle Proliferative Cell Cell-Cycle Markers Proto-Oncogenes |
topic |
Ciclo Celular Célula Proliferativa Marcadores Do Ciclo Celular Proto-Oncogenes Cellular Cycle Proliferative Cell Cell-Cycle Markers Proto-Oncogenes |
description |
The regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigos, Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbc.inca.gov.br/index.php/revista/article/view/2983 10.32635/2176-9745.RBC.1994v40n3.2983 |
url |
https://rbc.inca.gov.br/index.php/revista/article/view/2983 |
identifier_str_mv |
10.32635/2176-9745.RBC.1994v40n3.2983 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbc.inca.gov.br/index.php/revista/article/view/2983/1855 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
INCA |
publisher.none.fl_str_mv |
INCA |
dc.source.none.fl_str_mv |
Revista Brasileira de Cancerologia; Vol. 40 No. 3 (1994): July/Aug./Sept.; 141-147 Revista Brasileira de Cancerologia; Vol. 40 Núm. 3 (1994): jul./ago./sept.; 141-147 Revista Brasileira de Cancerologia; v. 40 n. 3 (1994): jul./ago./set.; 141-147 2176-9745 reponame:Revista Brasileira de Cancerologia (Online) instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) instacron:INCA |
instname_str |
Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) |
instacron_str |
INCA |
institution |
INCA |
reponame_str |
Revista Brasileira de Cancerologia (Online) |
collection |
Revista Brasileira de Cancerologia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) |
repository.mail.fl_str_mv |
rbc@inca.gov.br |
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1797042233796460544 |