Cell cycle: regulatory mechanisms and biochemical markers

Detalhes bibliográficos
Autor(a) principal: Rabenhorst, Silvia H.
Data de Publicação: 2023
Outros Autores: Burini, Roberto C., Schmitt, Fernando C. L.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Brasileira de Cancerologia (Online)
Texto Completo: https://rbc.inca.gov.br/index.php/revista/article/view/2983
Resumo: The regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability.
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spelling Cell cycle: regulatory mechanisms and biochemical markersCiclo celular: mecanismos reguladores e marcadores bioquímicosCiclo CelularCélula ProliferativaMarcadores Do Ciclo CelularProto-OncogenesCellular CycleProliferative CellCell-Cycle MarkersProto-OncogenesThe regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability.Os mecanismos reguladores do ciclo celular e sua inter-relação com fatores de crescimento, oncogenes e anti-oncogenes têm se constituído nas áreas de maior avanço no estudo da biologia do câncer. Continuamente estão sendo descobertas novas proteínas envolvidas no complexo mecanismo que desencadeia as diferentes fases do ciclo celular, como também os seus mecanismos de ativação. A descoberta dessas proteínas e co-fatores que regulam as transições G1/S e G2/M e que atuam na replicação do material bem como o papel dos proto-oncogenes dentro do ciclo celular permitiu aumentar o conhecimento sobre as células ciclizantes como tarnbém, permitiu que estas pudessem ser detectadas mesmo sem a visualização de figuras mitóticas. O desenvolvimento de anticorpos monoclonais contra estas proteínas tornou viável o estudo, em larga escala, de taxas de proliferação celular em diferentes neoplaslas, uma vez que substâncias como Ki67, polimerase alfa e PCNA - Ciclina somente são expressas em células em proliferação. A detecção de células proliferativas permite, através de sua contagem, estimar as taxas do crescimento neoplásico com conseqüente repercussão no prognóstico da doença e na terapêutica do paciente. Neste artigo são revistos os mecanismos de controle do ciclo celular bem como os métodos de detecção de células proliferativas, enfatizando a aplicação de marcadores pela sua praticidade e confiabilidade.INCA2023-06-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigos, Avaliado pelos paresapplication/pdfhttps://rbc.inca.gov.br/index.php/revista/article/view/298310.32635/2176-9745.RBC.1994v40n3.2983Revista Brasileira de Cancerologia; Vol. 40 No. 3 (1994): July/Aug./Sept.; 141-147Revista Brasileira de Cancerologia; Vol. 40 Núm. 3 (1994): jul./ago./sept.; 141-147Revista Brasileira de Cancerologia; v. 40 n. 3 (1994): jul./ago./set.; 141-1472176-9745reponame:Revista Brasileira de Cancerologia (Online)instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)instacron:INCAporhttps://rbc.inca.gov.br/index.php/revista/article/view/2983/1855https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRabenhorst, Silvia H.Burini, Roberto C. Schmitt, Fernando C. L. 2023-06-19T13:30:44Zoai:rbc.inca.gov.br:article/2983Revistahttps://rbc.inca.gov.br/index.php/revistaPUBhttps://rbc.inca.gov.br/index.php/revista/oairbc@inca.gov.br0034-71162176-9745opendoar:2023-06-19T13:30:44Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)false
dc.title.none.fl_str_mv Cell cycle: regulatory mechanisms and biochemical markers
Ciclo celular: mecanismos reguladores e marcadores bioquímicos
title Cell cycle: regulatory mechanisms and biochemical markers
spellingShingle Cell cycle: regulatory mechanisms and biochemical markers
Rabenhorst, Silvia H.
Ciclo Celular
Célula Proliferativa
Marcadores Do Ciclo Celular
Proto-Oncogenes
Cellular Cycle
Proliferative Cell
Cell-Cycle Markers
Proto-Oncogenes
title_short Cell cycle: regulatory mechanisms and biochemical markers
title_full Cell cycle: regulatory mechanisms and biochemical markers
title_fullStr Cell cycle: regulatory mechanisms and biochemical markers
title_full_unstemmed Cell cycle: regulatory mechanisms and biochemical markers
title_sort Cell cycle: regulatory mechanisms and biochemical markers
author Rabenhorst, Silvia H.
author_facet Rabenhorst, Silvia H.
Burini, Roberto C.
Schmitt, Fernando C. L.
author_role author
author2 Burini, Roberto C.
Schmitt, Fernando C. L.
author2_role author
author
dc.contributor.author.fl_str_mv Rabenhorst, Silvia H.
Burini, Roberto C.
Schmitt, Fernando C. L.
dc.subject.por.fl_str_mv Ciclo Celular
Célula Proliferativa
Marcadores Do Ciclo Celular
Proto-Oncogenes
Cellular Cycle
Proliferative Cell
Cell-Cycle Markers
Proto-Oncogenes
topic Ciclo Celular
Célula Proliferativa
Marcadores Do Ciclo Celular
Proto-Oncogenes
Cellular Cycle
Proliferative Cell
Cell-Cycle Markers
Proto-Oncogenes
description The regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability.
publishDate 2023
dc.date.none.fl_str_mv 2023-06-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigos, Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbc.inca.gov.br/index.php/revista/article/view/2983
10.32635/2176-9745.RBC.1994v40n3.2983
url https://rbc.inca.gov.br/index.php/revista/article/view/2983
identifier_str_mv 10.32635/2176-9745.RBC.1994v40n3.2983
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbc.inca.gov.br/index.php/revista/article/view/2983/1855
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv INCA
publisher.none.fl_str_mv INCA
dc.source.none.fl_str_mv Revista Brasileira de Cancerologia; Vol. 40 No. 3 (1994): July/Aug./Sept.; 141-147
Revista Brasileira de Cancerologia; Vol. 40 Núm. 3 (1994): jul./ago./sept.; 141-147
Revista Brasileira de Cancerologia; v. 40 n. 3 (1994): jul./ago./set.; 141-147
2176-9745
reponame:Revista Brasileira de Cancerologia (Online)
instname:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
instacron:INCA
instname_str Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
instacron_str INCA
institution INCA
reponame_str Revista Brasileira de Cancerologia (Online)
collection Revista Brasileira de Cancerologia (Online)
repository.name.fl_str_mv Revista Brasileira de Cancerologia (Online) - Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
repository.mail.fl_str_mv rbc@inca.gov.br
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