The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1301 |
Resumo: | DNA is the biopolymer most studied by scanning probe methods, and it is now possible to obtain reliable and reproducible images of DNA using atomic force microscopy (AFM). AFM has been extensively used to elucidate morphological changes to DNA structure, such as the formation of knots, nicks, supercoiling and bends. The mitochondrial or kinetoplast DNA (kDNA) of trypanosomatids is the most unusual DNA found in nature, being unique in organization and replication. The kDNA is composed of thousands of topologically interlocked DNA circles that form a giant network. To understand the biological significance of the kinetoplast DNA, it is necessary to learn more about its structure. In the present work, we used two procedures to prepare kDNA networks of Crithidia fasciculata for observation by AFM. Because AFM allows for the examination of kDNA at high resolution, we were able to identify regions of overlapping kDNA molecules and sites where several molecules cross. This found support the earlier described kDNA structural organization as composed by interlocked circles. We also observed an intricate high-density height pattern around the periphery of the network of C. fasciculata, which appears to be a bundle of DNA fibers that organizes the border of the network. Our present data confirm that AFM is a powerful tool to study the structural organization of biological samples, including complex arrays of DNA such as kDNA, and can be useful in revealing new details of structures previously visualized by other means. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy20112011-12-13T20:25:19Z2011-12-13T20:25:19ZDNA is the biopolymer most studied by scanning probe methods, and it is now possible to obtain reliable and reproducible images of DNA using atomic force microscopy (AFM). AFM has been extensively used to elucidate morphological changes to DNA structure, such as the formation of knots, nicks, supercoiling and bends. The mitochondrial or kinetoplast DNA (kDNA) of trypanosomatids is the most unusual DNA found in nature, being unique in organization and replication. The kDNA is composed of thousands of topologically interlocked DNA circles that form a giant network. To understand the biological significance of the kinetoplast DNA, it is necessary to learn more about its structure. In the present work, we used two procedures to prepare kDNA networks of Crithidia fasciculata for observation by AFM. Because AFM allows for the examination of kDNA at high resolution, we were able to identify regions of overlapping kDNA molecules and sites where several molecules cross. This found support the earlier described kDNA structural organization as composed by interlocked circles. We also observed an intricate high-density height pattern around the periphery of the network of C. fasciculata, which appears to be a bundle of DNA fibers that organizes the border of the network. Our present data confirm that AFM is a powerful tool to study the structural organization of biological samples, including complex arrays of DNA such as kDNA, and can be useful in revealing new details of structures previously visualized by other means.7 p. : il.Submitted by Cintia Machado (cpmachado@yahoo.com.br) on 2011-08-29T13:44:23Z No. of bitstreams: 1 2011_CavalcantiGonçalvesCosta.pdf: 856729 bytes, checksum: 8f0d518bc80e1e46aaa45561bd69215e (MD5)Approved for entry into archive by Catarina Soares(cfsoares@inmetro.gov.br) on 2011-12-13T20:25:19Z (GMT) No. of bitstreams: 1 2011_CavalcantiGonçalvesCosta.pdf: 856729 bytes, checksum: 8f0d518bc80e1e46aaa45561bd69215e (MD5)Made available in DSpace on 2011-12-13T20:25:19Z (GMT). No. of bitstreams: 1 2011_CavalcantiGonçalvesCosta.pdf: 856729 bytes, checksum: 8f0d518bc80e1e46aaa45561bd69215e (MD5) Previous issue date: 2011enghttp://hdl.handle.net/10926/1301DMD_hdl_10926/1301CAVALCANTI, Danielle Pereira et al. The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy. Micron, v. 42, p. 553-559, 2011.Cavalcanti, Danielle PereiraGonçalves, Daniela LeãoCosta, Lilian TerezinhaSouza, Wanderley deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROCavalcanti_2011.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1301/1/Cavalcanti_2011.pdfapplication/pdf856729http://xrepo01s.inmetro.gov.br/bitstream/10926/1301/1/Cavalcanti_2011.pdf8f0d518bc80e1e46aaa45561bd69215eMD510926_1301_1Cavalcanti_2011.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1301/6/Cavalcanti_2011.pdf.txttext/plain31903http://xrepo01s.inmetro.gov.br/bitstream/10926/1301/6/Cavalcanti_2011.pdf.txta3a80bb5adf27f2ba62d1c739d385ae6MD510926_1301_62024-06-10T15:24:39Zoai:xrepo01s.inmetro.gov.br:10926/1301Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2012-10-23T16:38:37Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
title |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
spellingShingle |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy Cavalcanti, Danielle Pereira |
title_short |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
title_full |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
title_fullStr |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
title_full_unstemmed |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
title_sort |
The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy |
author |
Cavalcanti, Danielle Pereira |
author_facet |
Cavalcanti, Danielle Pereira Gonçalves, Daniela Leão Costa, Lilian Terezinha Souza, Wanderley de |
author_role |
author |
author2 |
Gonçalves, Daniela Leão Costa, Lilian Terezinha Souza, Wanderley de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cavalcanti, Danielle Pereira Gonçalves, Daniela Leão Costa, Lilian Terezinha Souza, Wanderley de |
description |
DNA is the biopolymer most studied by scanning probe methods, and it is now possible to obtain reliable and reproducible images of DNA using atomic force microscopy (AFM). AFM has been extensively used to elucidate morphological changes to DNA structure, such as the formation of knots, nicks, supercoiling and bends. The mitochondrial or kinetoplast DNA (kDNA) of trypanosomatids is the most unusual DNA found in nature, being unique in organization and replication. The kDNA is composed of thousands of topologically interlocked DNA circles that form a giant network. To understand the biological significance of the kinetoplast DNA, it is necessary to learn more about its structure. In the present work, we used two procedures to prepare kDNA networks of Crithidia fasciculata for observation by AFM. Because AFM allows for the examination of kDNA at high resolution, we were able to identify regions of overlapping kDNA molecules and sites where several molecules cross. This found support the earlier described kDNA structural organization as composed by interlocked circles. We also observed an intricate high-density height pattern around the periphery of the network of C. fasciculata, which appears to be a bundle of DNA fibers that organizes the border of the network. Our present data confirm that AFM is a powerful tool to study the structural organization of biological samples, including complex arrays of DNA such as kDNA, and can be useful in revealing new details of structures previously visualized by other means. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.available.fl_str_mv |
2011-12-13T20:25:19Z |
dc.date.accessioned.fl_str_mv |
2011-12-13T20:25:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1301 DMD_hdl_10926/1301 |
dc.identifier.citation.fl_str_mv |
CAVALCANTI, Danielle Pereira et al. The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy. Micron, v. 42, p. 553-559, 2011. |
url |
http://hdl.handle.net/10926/1301 |
identifier_str_mv |
DMD_hdl_10926/1301 CAVALCANTI, Danielle Pereira et al. The structure of the kinetoplast DNA network of Crithidia fasciculata revealed by atomic force microscopy. Micron, v. 42, p. 553-559, 2011. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf text/plain |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INMETRO instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) instacron:INMETRO |
instname_str |
Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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