Atomic force microscopy characterization of an electrochemical DNA-biosensor

Detalhes bibliográficos
Autor(a) principal: Paquim, Ana Maria Chiorcea
Data de Publicação: 2004
Outros Autores: Brett, Ana Maria Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5131
https://doi.org/10.1016/j.bioelechem.2003.09.029
Resumo: Electrode surface characteristics represent an important aspect on the construction of sensitive DNA electrochemical biosensors for rapid detection of DNA interaction and damage. Two different immobilization procedures of double-stranded DNA (dsDNA) at the surface of a HOPG electrode were evaluated by MAC mode AFM performed in air. A thin dsDNA adsorbed film forming a network structure with holes exposing the electrode surface and a thick dsDNA film completely covering the electrode surface, presenting a much rougher structure, were investigated. The DNA surface characteristics and structure are discussed with respect to the degree of surface coverage.
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spelling Atomic force microscopy characterization of an electrochemical DNA-biosensorDNA-biosensorAtomic force microscopyElectrode surface characteristics represent an important aspect on the construction of sensitive DNA electrochemical biosensors for rapid detection of DNA interaction and damage. Two different immobilization procedures of double-stranded DNA (dsDNA) at the surface of a HOPG electrode were evaluated by MAC mode AFM performed in air. A thin dsDNA adsorbed film forming a network structure with holes exposing the electrode surface and a thick dsDNA film completely covering the electrode surface, presenting a much rougher structure, were investigated. The DNA surface characteristics and structure are discussed with respect to the degree of surface coverage.http://www.sciencedirect.com/science/article/B6W72-4BYNKV5-1/1/e3b9889d1883556b572dab2937f4d7452004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5131http://hdl.handle.net/10316/5131https://doi.org/10.1016/j.bioelechem.2003.09.029engBioelectrochemistry. 63:1-2 (2004) 229-232Paquim, Ana Maria ChiorceaBrett, Ana Maria Oliveirainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:48:50Zoai:estudogeral.uc.pt:10316/5131Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:38.680961Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Atomic force microscopy characterization of an electrochemical DNA-biosensor
title Atomic force microscopy characterization of an electrochemical DNA-biosensor
spellingShingle Atomic force microscopy characterization of an electrochemical DNA-biosensor
Paquim, Ana Maria Chiorcea
DNA-biosensor
Atomic force microscopy
title_short Atomic force microscopy characterization of an electrochemical DNA-biosensor
title_full Atomic force microscopy characterization of an electrochemical DNA-biosensor
title_fullStr Atomic force microscopy characterization of an electrochemical DNA-biosensor
title_full_unstemmed Atomic force microscopy characterization of an electrochemical DNA-biosensor
title_sort Atomic force microscopy characterization of an electrochemical DNA-biosensor
author Paquim, Ana Maria Chiorcea
author_facet Paquim, Ana Maria Chiorcea
Brett, Ana Maria Oliveira
author_role author
author2 Brett, Ana Maria Oliveira
author2_role author
dc.contributor.author.fl_str_mv Paquim, Ana Maria Chiorcea
Brett, Ana Maria Oliveira
dc.subject.por.fl_str_mv DNA-biosensor
Atomic force microscopy
topic DNA-biosensor
Atomic force microscopy
description Electrode surface characteristics represent an important aspect on the construction of sensitive DNA electrochemical biosensors for rapid detection of DNA interaction and damage. Two different immobilization procedures of double-stranded DNA (dsDNA) at the surface of a HOPG electrode were evaluated by MAC mode AFM performed in air. A thin dsDNA adsorbed film forming a network structure with holes exposing the electrode surface and a thick dsDNA film completely covering the electrode surface, presenting a much rougher structure, were investigated. The DNA surface characteristics and structure are discussed with respect to the degree of surface coverage.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5131
http://hdl.handle.net/10316/5131
https://doi.org/10.1016/j.bioelechem.2003.09.029
url http://hdl.handle.net/10316/5131
https://doi.org/10.1016/j.bioelechem.2003.09.029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioelectrochemistry. 63:1-2 (2004) 229-232
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/PDF
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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