Complete genome sequence of the sugarcane nitrogen-fixing endophyte gluconacetobacter diazotrophicus PAL5

• Autor(a) principal: Bertalan, Marcelo
• Data de Publicação: 2010
• Outros Autores: Albano, Rodolpho Mattos, Pádua, Vânia Lúcia Muniz de, Rouws, Luc, Rojas, Cristian, Hemerly, Adriana Silva, Teixeira, Katia Regina dos Santos, Schwab, Stefan, Araujo, Jean Luiz Simões de, Oliveira, André Lopes de, França, Leonardo, Magalhães, Viviane de Souza, Alquéres, Sylvia Maria Campbell, Cardoso, Alexander Machado, Almeida, Welington Inacio de, Loureiro, Marcio Martins, Nogueira, Eduardo, Cidade, Daniela, Oliveira, Denise Neves de, Simão, Tatiana de Almeida, Macedo, Jacyara, Valadão, Ana, Dreschsel, Marcela Motta, Freitas, Flávia Alvim Dutra de, Vidal, Marcia Soares, Guedes, Helma Ventura, Rodrigues, Elisete, Meneses, Carlos, Bisch, Paulo Mascarello, Pozzer, Luciana, Figueiredo, Daniel Vazquez, Montano, Helena Guglielmi, Cunha Junior, Jadier de Oliveira, Souza Filho, Gonçalo Apolinário de, Flores, Victor Martin Quintana, Ferreira, Beatriz, Branco, Alan, Gonzalez, Paula, Guillobel, Heloisa, Lemos, Melissa, Seibel, Luiz, Macedo, José Ronaldo de, Ferreira, Marcio Alves, Martins, Gilberto Sachetto, Coelho, Ana Maria Abrantes, Santos, Eidy, Amaral, Gilda, Borges, Anna Cristina Neves, Pacheco, Ana Beatriz Furlanetto, Carvalho, Daniela Palermo de, Santos, Leticia Miranda Lery, Rössle, Shaila Cintia Sykora, Ürmenyic, Turán Peter, Pereira, Alessandra Rael, Silva, Rosane, Rondinelli, Edson, Krüger, Wanda Maria Almeida von, Martins, Orlando Bonifácio, Baldani, José Ivo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INMETRO
• Texto Completo: http://hdl.handle.net/10926/1722

Background: Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results: Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion: The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.