Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tribular epithelial cells after unilateral ureteral obstruction

• Autor(a) principal: Barreira, André Luis
• Data de Publicação: 2009
• Outros Autores: Takiya, Christina Maeda, Castiglione, Raquel Carvalho, Gutierrez, Tatiana Maron, Barbosa, Carolina M. L., Ornellas, Débora dos Santos, Verdoorn, Karine da Silva, Pascarelli, Bernardo Miguel de Oliveira, Borojevic, Radovan, Lamas, Marcelo Einicker, Leite Júnior, Maurilo de Nazaré de Lima, Morales, Marcelo Marcos, Vieyra, Adalberto Ramon
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional do INMETRO
• Texto Completo: http://hdl.handle.net/10926/1546

The growing number of patients suffering from chronic renal disease is a challenge for the development of innovative therapies. Benefits of cell therapy in acute renal diseases in animal models have been reported but seldom for chronic lesions. We present evidence for the improvement of renal morphology in a model of tubulointerstitial fibrosis. Wistar rats were submitted to unilateral ureteral obstruction (UUO), treated with bone-marrow mononuclear cells (UUO+BMMC) infused via the cava vein, and killed on day 14. Labeled BMMC were seen in renal tissue after 7 days in the group UUO+BMMC. UUO+BMMC also showed a reduction in ED1+ cells and tubular apoptotic cells together with enhanced tubular proliferation. Myofibroblasts were also reduced after BMMC which is consistent with a decrease in collagen deposition (picro Sirius staining) and RT-PCR data showing lower levels of procollagen-I mRNA. Simultaneously, nestin+ cells increased in the interstitium and decreased in the tubules. Double stained nestin+/α-SMA+ cells were present only in the interstitium, and their levels did not change after BMMC infusion. These data indicate a renoprotective effect of BMMC through increased tubular cell regeneration, inhibition of tubular cell apoptosis and partially blocking of the inflammatory and fibrotic events that occur after unilateral ureteral obstruction.