Purificação de IgG de codorna a partir de soro e gema de ovos

Detalhes bibliográficos
Autor(a) principal: Oliveira, Daniela Duarte de
Data de Publicação: 2010
Outros Autores: Resende, José Sérgio de, Folgueras-Flatschart, Aurea Valadares, Abreu, Josiane Tavares de, Flatschart, Roberto Becht, Martins, Nelson Rodrigo da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional do INMETRO
Texto Completo: http://hdl.handle.net/10926/1094
Resumo: The purification of Japanese quail IgG from serum was performed using caprilic acid and ammonium sulfate, and from egg yolk using PEG-6000 and ethanol. After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples. The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard. So, these methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays.
id INMT_a322c114b616b30ddb5e42f6f5a54425
oai_identifier_str oai:xrepo01s.inmetro.gov.br:10926/1094
network_acronym_str INMT
network_name_str Repositório Institucional do INMETRO
repository_id_str
spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePurificação de IgG de codorna a partir de soro e gema de ovos20102011-06-16T17:33:37Z2011-06-16T17:33:37ZThe purification of Japanese quail IgG from serum was performed using caprilic acid and ammonium sulfate, and from egg yolk using PEG-6000 and ethanol. After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples. The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard. So, these methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays.3 p. : il.Submitted by Bruna Fernandes de farias (bruna.fernandes_farias@yahoo.com.br) on 2011-05-04T14:01:17Z No. of bitstreams: 1 2010_OliveiraResende.pdf: 341663 bytes, checksum: bb078bbd70457429aeb2c367ea508ab8 (MD5)Rejected by Catarina Soares(cfsoares@inmetro.gov.br), reason: on 2011-05-06T13:26:34Z (GMT)Submitted by Bruna Fernandes de farias (bruna.fernandes_farias@yahoo.com.br) on 2011-05-06T13:30:17Z No. of bitstreams: 1 2010_OliveiraResende.pdf: 341663 bytes, checksum: bb078bbd70457429aeb2c367ea508ab8 (MD5)Approved for entry into archive by Catarina Soares(cfsoares@inmetro.gov.br) on 2011-06-16T17:33:37Z (GMT) No. of bitstreams: 1 2010_OliveiraResende.pdf: 341663 bytes, checksum: bb078bbd70457429aeb2c367ea508ab8 (MD5)Made available in DSpace on 2011-06-16T17:33:37Z (GMT). No. of bitstreams: 1 2010_OliveiraResende.pdf: 341663 bytes, checksum: bb078bbd70457429aeb2c367ea508ab8 (MD5) Previous issue date: 2010porhttp://hdl.handle.net/10926/1094DMD_hdl_10926/1094OLIVEIRA, D. D. et al. Purificação de IgG de codorna a partir de soro e gema de ovos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 62, n. 2, p. 492-494, 2010.Oliveira, Daniela Duarte deResende, José Sérgio deFolgueras-Flatschart, Aurea ValadaresAbreu, Josiane Tavares deFlatschart, Roberto BechtMartins, Nelson Rodrigo da Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROOliveira_2010.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1094/1/Oliveira_2010.pdfapplication/pdf341663http://xrepo01s.inmetro.gov.br/bitstream/10926/1094/1/Oliveira_2010.pdfbb078bbd70457429aeb2c367ea508ab8MD510926_1094_1Oliveira_2010.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1094/6/Oliveira_2010.pdf.txttext/plain10460http://xrepo01s.inmetro.gov.br/bitstream/10926/1094/6/Oliveira_2010.pdf.txt6164f22452ba04ad715a3f7faf3d143eMD510926_1094_62024-06-10T15:24:34Zoai:xrepo01s.inmetro.gov.br:10926/1094Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2013-05-15T13:01:24Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false
dc.title.none.fl_str_mv Purificação de IgG de codorna a partir de soro e gema de ovos
title Purificação de IgG de codorna a partir de soro e gema de ovos
spellingShingle Purificação de IgG de codorna a partir de soro e gema de ovos
Oliveira, Daniela Duarte de
title_short Purificação de IgG de codorna a partir de soro e gema de ovos
title_full Purificação de IgG de codorna a partir de soro e gema de ovos
title_fullStr Purificação de IgG de codorna a partir de soro e gema de ovos
title_full_unstemmed Purificação de IgG de codorna a partir de soro e gema de ovos
title_sort Purificação de IgG de codorna a partir de soro e gema de ovos
author Oliveira, Daniela Duarte de
author_facet Oliveira, Daniela Duarte de
Resende, José Sérgio de
Folgueras-Flatschart, Aurea Valadares
Abreu, Josiane Tavares de
Flatschart, Roberto Becht
Martins, Nelson Rodrigo da Silva
author_role author
author2 Resende, José Sérgio de
Folgueras-Flatschart, Aurea Valadares
Abreu, Josiane Tavares de
Flatschart, Roberto Becht
Martins, Nelson Rodrigo da Silva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Daniela Duarte de
Resende, José Sérgio de
Folgueras-Flatschart, Aurea Valadares
Abreu, Josiane Tavares de
Flatschart, Roberto Becht
Martins, Nelson Rodrigo da Silva
description The purification of Japanese quail IgG from serum was performed using caprilic acid and ammonium sulfate, and from egg yolk using PEG-6000 and ethanol. After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples. The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard. So, these methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.available.fl_str_mv 2011-06-16T17:33:37Z
dc.date.accessioned.fl_str_mv 2011-06-16T17:33:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10926/1094
DMD_hdl_10926/1094
dc.identifier.citation.fl_str_mv OLIVEIRA, D. D. et al. Purificação de IgG de codorna a partir de soro e gema de ovos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 62, n. 2, p. 492-494, 2010.
url http://hdl.handle.net/10926/1094
identifier_str_mv DMD_hdl_10926/1094
OLIVEIRA, D. D. et al. Purificação de IgG de codorna a partir de soro e gema de ovos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 62, n. 2, p. 492-494, 2010.
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.bitstream.fl_str_mv application/pdf
text/plain
dc.source.none.fl_str_mv reponame:Repositório Institucional do INMETRO
instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)
instacron:INMETRO
instname_str Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)
instacron_str INMETRO
institution INMETRO
reponame_str Repositório Institucional do INMETRO
collection Repositório Institucional do INMETRO
repository.name.fl_str_mv Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)
repository.mail.fl_str_mv
_version_ 1801499965518774272

Registros relacionados