Development and validation of a method for the analysis of ochratoxin a in roasted coffee

• Autor(a) principal: Bandeira, Raquel Duarte da Costa Cunha
• Data de Publicação: 2011
• Outros Autores: Uekane, Thais Matsue, Cunha, Carolina P. da, Geaquinto, Luths Raquel de Oliveira, Cunha, Valnei Smarçaro da, Caixeiro, Janaína Marques Rodrigues, Godoy, Ronoel Luiz de Oliveira, Cruz, Marcus Henrique Campino de la
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INMETRO
• Texto Completo: http://hdl.handle.net/10926/1539

A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.