Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

• Autor(a) principal: Bandeira,Raquel Duarte da Costa Cunha
• Data de Publicação: 2012
• Outros Autores: Uekane,Thaís Matsue, Cunha,Carolina Passos da, Cunha,Valnei Smarçaro da, Rodrigues,Janaína Marques, Godoy,Ronoel Luiz de Oliveira, la Cruz,Marcus Henrique Campino de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Food Science and Technology (Campinas)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400021
• topic: coffee; mycotoxin; validation; HPLC; MS/MS

A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g-1. Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g-1 and 3.0 ng.g-¹, respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.