Proteomic analysis of the ventral disc of Giardia lamblia
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1923 |
Resumo: | Background: Giardia lamblia is a multiflagellated protozoan that inhabits the small intestine of vertebrates, causing giardiasis. To colonize the small intestine, the trophozoites form of the parasite remains attached to intestinal epithelial cells by means of cytoskeletal elements that form a structure known as the ventral disc. Previous studies have shown that the ventral disc is made of tubulin and giardins. Results: To obtain further information on the composition of the ventral disc, we developed a new protocol and evaluated the purity of the isolation by transmission electron microscopy. Using 1D- and 2D-PAGE and mass spectrometry, we identified proteins with functions associated with the disc. In addition to finding tubulin and giardin, proteins known to be associated with the ventral disc, we also identified proteins annotated in the Giardia genome, but whose function was previously unknown. Conclusions: The isolation of the ventral disc shown in this work, compared to previously published protocols, proved to be more efficient. Proteomic analysis showed the presence of several proteins whose further characterization may help in the elucidation of the mechanisms involved in the attachment of the protozoan to epithelial cells. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleProteomic analysis of the ventral disc of Giardia lamblia20122013-01-16T11:57:49Z2013-01-16T11:57:49ZBackground: Giardia lamblia is a multiflagellated protozoan that inhabits the small intestine of vertebrates, causing giardiasis. To colonize the small intestine, the trophozoites form of the parasite remains attached to intestinal epithelial cells by means of cytoskeletal elements that form a structure known as the ventral disc. Previous studies have shown that the ventral disc is made of tubulin and giardins. Results: To obtain further information on the composition of the ventral disc, we developed a new protocol and evaluated the purity of the isolation by transmission electron microscopy. Using 1D- and 2D-PAGE and mass spectrometry, we identified proteins with functions associated with the disc. In addition to finding tubulin and giardin, proteins known to be associated with the ventral disc, we also identified proteins annotated in the Giardia genome, but whose function was previously unknown. Conclusions: The isolation of the ventral disc shown in this work, compared to previously published protocols, proved to be more efficient. Proteomic analysis showed the presence of several proteins whose further characterization may help in the elucidation of the mechanisms involved in the attachment of the protozoan to epithelial cells.9 p. : il., tab.Submitted by Lívia Oliveira (livia.oliveira2@hotmail.com) on 2012-08-16T12:58:48Z No. of bitstreams: 1 Souza3_2012.pdf: 587185 bytes, checksum: 6d759e9045dcb412c255784ddc2fcb43 (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2013-01-16T11:57:49Z (GMT) No. of bitstreams: 1 Souza3_2012.pdf: 587185 bytes, checksum: 6d759e9045dcb412c255784ddc2fcb43 (MD5)Made available in DSpace on 2013-01-16T11:57:49Z (GMT). No. of bitstreams: 1 Souza3_2012.pdf: 587185 bytes, checksum: 6d759e9045dcb412c255784ddc2fcb43 (MD5) Previous issue date: 2012enghttp://hdl.handle.net/10926/1923DMD_hdl_10926/1923LOURENÇO, Daniela et al. Proteomic analysis of the ventral disc of Giardia lamblia. Bio Med Central Research Notes, v. 5, n. 41, 2012.Lourenço, Daniela CamposAndrade, Iamara da SilvaTerra, Letícia LabatiGuimarães, Patricia RamosZingali, Russolina BenedetaSouza, Wanderley deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROLouren%c3%a7o_2012.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1923/1/Louren%c3%a7o_2012.pdfapplication/pdf587185http://xrepo01s.inmetro.gov.br/bitstream/10926/1923/1/Louren%c3%a7o_2012.pdf6d759e9045dcb412c255784ddc2fcb43MD510926_1923_1Louren%c3%a7o_2012.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1923/6/Louren%c3%a7o_2012.pdf.txttext/plain33425http://xrepo01s.inmetro.gov.br/bitstream/10926/1923/6/Louren%c3%a7o_2012.pdf.txtcfbd75e63cefed22f907dd2f057699f2MD510926_1923_62024-06-10T15:24:56Zoai:xrepo01s.inmetro.gov.br:10926/1923Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2013-03-28T17:59:59Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Proteomic analysis of the ventral disc of Giardia lamblia |
title |
Proteomic analysis of the ventral disc of Giardia lamblia |
spellingShingle |
Proteomic analysis of the ventral disc of Giardia lamblia Lourenço, Daniela Campos |
title_short |
Proteomic analysis of the ventral disc of Giardia lamblia |
title_full |
Proteomic analysis of the ventral disc of Giardia lamblia |
title_fullStr |
Proteomic analysis of the ventral disc of Giardia lamblia |
title_full_unstemmed |
Proteomic analysis of the ventral disc of Giardia lamblia |
title_sort |
Proteomic analysis of the ventral disc of Giardia lamblia |
author |
Lourenço, Daniela Campos |
author_facet |
Lourenço, Daniela Campos Andrade, Iamara da Silva Terra, Letícia Labati Guimarães, Patricia Ramos Zingali, Russolina Benedeta Souza, Wanderley de |
author_role |
author |
author2 |
Andrade, Iamara da Silva Terra, Letícia Labati Guimarães, Patricia Ramos Zingali, Russolina Benedeta Souza, Wanderley de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lourenço, Daniela Campos Andrade, Iamara da Silva Terra, Letícia Labati Guimarães, Patricia Ramos Zingali, Russolina Benedeta Souza, Wanderley de |
description |
Background: Giardia lamblia is a multiflagellated protozoan that inhabits the small intestine of vertebrates, causing giardiasis. To colonize the small intestine, the trophozoites form of the parasite remains attached to intestinal epithelial cells by means of cytoskeletal elements that form a structure known as the ventral disc. Previous studies have shown that the ventral disc is made of tubulin and giardins. Results: To obtain further information on the composition of the ventral disc, we developed a new protocol and evaluated the purity of the isolation by transmission electron microscopy. Using 1D- and 2D-PAGE and mass spectrometry, we identified proteins with functions associated with the disc. In addition to finding tubulin and giardin, proteins known to be associated with the ventral disc, we also identified proteins annotated in the Giardia genome, but whose function was previously unknown. Conclusions: The isolation of the ventral disc shown in this work, compared to previously published protocols, proved to be more efficient. Proteomic analysis showed the presence of several proteins whose further characterization may help in the elucidation of the mechanisms involved in the attachment of the protozoan to epithelial cells. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.available.fl_str_mv |
2013-01-16T11:57:49Z |
dc.date.accessioned.fl_str_mv |
2013-01-16T11:57:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1923 DMD_hdl_10926/1923 |
dc.identifier.citation.fl_str_mv |
LOURENÇO, Daniela et al. Proteomic analysis of the ventral disc of Giardia lamblia. Bio Med Central Research Notes, v. 5, n. 41, 2012. |
url |
http://hdl.handle.net/10926/1923 |
identifier_str_mv |
DMD_hdl_10926/1923 LOURENÇO, Daniela et al. Proteomic analysis of the ventral disc of Giardia lamblia. Bio Med Central Research Notes, v. 5, n. 41, 2012. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf text/plain |
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reponame:Repositório Institucional do INMETRO instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) instacron:INMETRO |
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Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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