Transferrin polymorphism in Central Amazon populations of pescada,Plagioscion squamosissimus

• Autor(a) principal: Teixeira, Aylton Saturnino
• Data de Publicação: 2002
• Outros Autores: Jamieson, Alan, Raposo, José Carlos Paula
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/16410
• topic: Transferrin; Transferrin; Animals Experiment; Chi Square Test; Cluster Analysis; Contingency Table; Controlled Study; Demography; Gel Electrophoresis; Gene Frequency; Gene Isolation; Gene Locus; Genetic Analysis; Genetic Distance; Genetic Marker; Polymorphism, Genetic; Genetic Stability; Genetic Variability; Genotype; Heterozygosity; Histogram; Homozygote; Nonhuman; Phylogenetic Tree; Plagioscion Squamosissimus; Population Differentiation; Population Structure; Statistical Analysis; Teleost; Transferrin Blood Level; Allele; Animals; Brasil; Ecosystem; Fish; Gene Frequency; Genetics; Animalsia; Plagioscion; Plagioscion Squamosissimum; Plagioscion Squamosissimus; Teleostei; Alleles; Animal; Brasil; Ecosystem; Electrophoresis, Starch Gel; Fishes; Gene Frequency; Genotype; Polymorphism, Genetic; Transferrin; Variation (genetics)

Blood plasma of 253 specimens from eight population samples of the sciaenid fish, pescada (Plagioscion squamosissimus), caught from four sites in the Central Amazon, was tested for molecular variants of transferrin. Starch gel electrophoresis was used to distinguish six species of transferrin molecules; 12 of the 21 theoretically possible genotypes were found. There were highly significant departures from genetic equilibrium in seven of the eight population samples (chi-square (χ2) test for Hardy-Weinberg expectations) due to an excess of homozygotes and a corresponding deficiency of heterozygotes. A dendrogram based on UPGMA cluster analysis of genetic distances at the transferrin gene locus, estimated among the population samples and statistical analyses of the distribution of Tf allele frequencies, indicated three genetically discreet sub-populations of P. squamosissimus. The three sub-populations, "Careiro/Iranduba", "Coari" and "Tefe", were found to have high frequencies of alleles Tf2, Tf4 and Tf3, respectively. This genetic instability may be attributed to genetically discreet "allopatric stocklets", which diverged during past isolation.