Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey

• Autor(a) principal: Pimentel, Renah Boanerges de Queiroz
• Data de Publicação: 2013
• Outros Autores: Costa, Cristóvão Alves da, Albuquerque, Patrícia Melchionna, Duvoisin Júnior, Sérgio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/16056
• topic: Agar; Apigenin; Flavonoid; Phenol Derivative; Rutoside; Agar Diffusion; Antibacterial Activity; Apis; Bacterial Growth; Broth Dilution; Chromatography; Controlled Study; Entomopathogenic Bacterium; Escherichia Coli; Gram Negative Bacterium; Gram Positive Bacterium; High Performance Liquid Chromatography; Honey; Klebsiella; Liquid Liquid Extraction; Nonhuman; Proteus Vulgaris; Salmonella Paratyphi; Season; Shigella Sonnei; Staphylococcus Aureus; Stingless Bee; Animal; Anti-bacterial Agents; Apitherapy; Bees; Chromatography, High Pressure Liquid; Gram-negative Bacteria; Gram-positive Bacteria; Honey; Phenols; Rutin

Background: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds.Methods: Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm.Results: Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee.Conclusions: Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth. © 2013 Pimentel et al.; licensee BioMed Central Ltd.