MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/14658 |
Resumo: | Cryptococcosis is considered endemic in Amazonas state, occurring more frequently in individuals with AIDS, who are predominantly infected by Cryptococcus neoformans molecular type VNI. Infections by Cryptococcus gattii VGII predominate in immunocompetent hosts from the American continent and are associated with outbreaks in North America, particularly the subtypes VGIIa and VGIIb, which are also present in the Brazilian Amazon region. Despite few environmental studies, several aspects of the molecular epidemiology of this disease in Amazonas remain unclear, including the limited use of multilocus sequence typing (MLST) to evaluate the genetic population structure of clinical isolates, mainly C. neoformans. Therefore, we used MLST to identify the sequence types of 38 clinical isolates of C. neoformans VNI and C. gattii VGII and used phylogenetic analysis to evaluate their genetic relationship to global isolates. Records of 30 patients were analyzed to describe the current scenario of cryptococcosis in the region and their associations with the different subtypes. Broth microdilution was also performed to determine the susceptibility profile to the antifungals amphotericin B, fluconazole and itraconazole. MLST identified that patients with HIV (n = 26) were exclusively affected by VNI strains with ST93, and among the VGII strains (n = 4), three STs (ST5, ST172 and the new ST445) were identified. An in-hospital lethality of 54% was observed in the HIV group, and there were no significant differences in the clinical aspects of the disease between the HIV and non-HIV groups of patients. In addition, all isolates were susceptible to the antifungals tested. Therefore, in Amazonas state, VNI isolates are a genetically monotypic group, with ST93 being highly important in HIV individuals. © 2018 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
id |
INPA-2_46e9bd6391f11768be8be1082a4bb15f |
---|---|
oai_identifier_str |
oai:repositorio:1/14658 |
network_acronym_str |
INPA-2 |
network_name_str |
Repositório Institucional do INPA |
repository_id_str |
|
spelling |
Rocha, Diego Fernando SilvaCruz, Kátia SantanaSilva Santos, Carla Silvana daStephanny Fernandes Menescal, LizandraSilva Neto, João Ricardo daPinheiro, Silviane BezerraSilva, Lucyane MendesTrilles, LucianaSouza, João Vicente Braga de2020-04-24T16:59:59Z2020-04-24T16:59:59Z2018https://repositorio.inpa.gov.br/handle/1/1465810.1371/journal.pone.0197841Cryptococcosis is considered endemic in Amazonas state, occurring more frequently in individuals with AIDS, who are predominantly infected by Cryptococcus neoformans molecular type VNI. Infections by Cryptococcus gattii VGII predominate in immunocompetent hosts from the American continent and are associated with outbreaks in North America, particularly the subtypes VGIIa and VGIIb, which are also present in the Brazilian Amazon region. Despite few environmental studies, several aspects of the molecular epidemiology of this disease in Amazonas remain unclear, including the limited use of multilocus sequence typing (MLST) to evaluate the genetic population structure of clinical isolates, mainly C. neoformans. Therefore, we used MLST to identify the sequence types of 38 clinical isolates of C. neoformans VNI and C. gattii VGII and used phylogenetic analysis to evaluate their genetic relationship to global isolates. Records of 30 patients were analyzed to describe the current scenario of cryptococcosis in the region and their associations with the different subtypes. Broth microdilution was also performed to determine the susceptibility profile to the antifungals amphotericin B, fluconazole and itraconazole. MLST identified that patients with HIV (n = 26) were exclusively affected by VNI strains with ST93, and among the VGII strains (n = 4), three STs (ST5, ST172 and the new ST445) were identified. An in-hospital lethality of 54% was observed in the HIV group, and there were no significant differences in the clinical aspects of the disease between the HIV and non-HIV groups of patients. In addition, all isolates were susceptible to the antifungals tested. Therefore, in Amazonas state, VNI isolates are a genetically monotypic group, with ST93 being highly important in HIV individuals. © 2018 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Volume 13, Número 6Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAmphotericin BFluconazoleItraconazoleAdultAmazonasAntifungal SusceptibilityBroth DilutionClinical Controlled StudyCryptococcosisCryptococcus GattiiCryptococcus NeoformansFemaleFungal StrainFungemiaFungus IsolationHumanMaleMolecular TypingMultilocus Sequence TypingNeurocryptococcosisNonhumanPhylogenyPopulation StructureAgedBrasilClassificationCryptococcosisCryptococcus NeoformansMicrobiological ExaminationMiddle AgedPhysiologyYoung AdultAdultAgedBrasilCryptococcosisCryptococcus NeoformansFemaleHumansMaleMiddle AgedMultilocus Sequence TypingMycological Typing TechniquesPhylogenyYoung AdultMLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS ONEengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf3157025https://repositorio.inpa.gov.br/bitstream/1/14658/1/artigo-inpa.pdffb56bab5e5dd5ee5f9df2a494390a313MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14658/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/146582020-07-14 09:19:11.543oai:repositorio:1/14658Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T13:19:11Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
title |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
spellingShingle |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil Rocha, Diego Fernando Silva Amphotericin B Fluconazole Itraconazole Adult Amazonas Antifungal Susceptibility Broth Dilution Clinical Controlled Study Cryptococcosis Cryptococcus Gattii Cryptococcus Neoformans Female Fungal Strain Fungemia Fungus Isolation Human Male Molecular Typing Multilocus Sequence Typing Neurocryptococcosis Nonhuman Phylogeny Population Structure Aged Brasil Classification Cryptococcosis Cryptococcus Neoformans Microbiological Examination Middle Aged Physiology Young Adult Adult Aged Brasil Cryptococcosis Cryptococcus Neoformans Female Humans Male Middle Aged Multilocus Sequence Typing Mycological Typing Techniques Phylogeny Young Adult |
title_short |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
title_full |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
title_fullStr |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
title_full_unstemmed |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
title_sort |
MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil |
author |
Rocha, Diego Fernando Silva |
author_facet |
Rocha, Diego Fernando Silva Cruz, Kátia Santana Silva Santos, Carla Silvana da Stephanny Fernandes Menescal, Lizandra Silva Neto, João Ricardo da Pinheiro, Silviane Bezerra Silva, Lucyane Mendes Trilles, Luciana Souza, João Vicente Braga de |
author_role |
author |
author2 |
Cruz, Kátia Santana Silva Santos, Carla Silvana da Stephanny Fernandes Menescal, Lizandra Silva Neto, João Ricardo da Pinheiro, Silviane Bezerra Silva, Lucyane Mendes Trilles, Luciana Souza, João Vicente Braga de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rocha, Diego Fernando Silva Cruz, Kátia Santana Silva Santos, Carla Silvana da Stephanny Fernandes Menescal, Lizandra Silva Neto, João Ricardo da Pinheiro, Silviane Bezerra Silva, Lucyane Mendes Trilles, Luciana Souza, João Vicente Braga de |
dc.subject.eng.fl_str_mv |
Amphotericin B Fluconazole Itraconazole Adult Amazonas Antifungal Susceptibility Broth Dilution Clinical Controlled Study Cryptococcosis Cryptococcus Gattii Cryptococcus Neoformans Female Fungal Strain Fungemia Fungus Isolation Human Male Molecular Typing Multilocus Sequence Typing Neurocryptococcosis Nonhuman Phylogeny Population Structure Aged Brasil Classification Cryptococcosis Cryptococcus Neoformans Microbiological Examination Middle Aged Physiology Young Adult Adult Aged Brasil Cryptococcosis Cryptococcus Neoformans Female Humans Male Middle Aged Multilocus Sequence Typing Mycological Typing Techniques Phylogeny Young Adult |
topic |
Amphotericin B Fluconazole Itraconazole Adult Amazonas Antifungal Susceptibility Broth Dilution Clinical Controlled Study Cryptococcosis Cryptococcus Gattii Cryptococcus Neoformans Female Fungal Strain Fungemia Fungus Isolation Human Male Molecular Typing Multilocus Sequence Typing Neurocryptococcosis Nonhuman Phylogeny Population Structure Aged Brasil Classification Cryptococcosis Cryptococcus Neoformans Microbiological Examination Middle Aged Physiology Young Adult Adult Aged Brasil Cryptococcosis Cryptococcus Neoformans Female Humans Male Middle Aged Multilocus Sequence Typing Mycological Typing Techniques Phylogeny Young Adult |
description |
Cryptococcosis is considered endemic in Amazonas state, occurring more frequently in individuals with AIDS, who are predominantly infected by Cryptococcus neoformans molecular type VNI. Infections by Cryptococcus gattii VGII predominate in immunocompetent hosts from the American continent and are associated with outbreaks in North America, particularly the subtypes VGIIa and VGIIb, which are also present in the Brazilian Amazon region. Despite few environmental studies, several aspects of the molecular epidemiology of this disease in Amazonas remain unclear, including the limited use of multilocus sequence typing (MLST) to evaluate the genetic population structure of clinical isolates, mainly C. neoformans. Therefore, we used MLST to identify the sequence types of 38 clinical isolates of C. neoformans VNI and C. gattii VGII and used phylogenetic analysis to evaluate their genetic relationship to global isolates. Records of 30 patients were analyzed to describe the current scenario of cryptococcosis in the region and their associations with the different subtypes. Broth microdilution was also performed to determine the susceptibility profile to the antifungals amphotericin B, fluconazole and itraconazole. MLST identified that patients with HIV (n = 26) were exclusively affected by VNI strains with ST93, and among the VGII strains (n = 4), three STs (ST5, ST172 and the new ST445) were identified. An in-hospital lethality of 54% was observed in the HIV group, and there were no significant differences in the clinical aspects of the disease between the HIV and non-HIV groups of patients. In addition, all isolates were susceptible to the antifungals tested. Therefore, in Amazonas state, VNI isolates are a genetically monotypic group, with ST93 being highly important in HIV individuals. © 2018 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-04-24T16:59:59Z |
dc.date.available.fl_str_mv |
2020-04-24T16:59:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/14658 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0197841 |
url |
https://repositorio.inpa.gov.br/handle/1/14658 |
identifier_str_mv |
10.1371/journal.pone.0197841 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 13, Número 6 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
PLoS ONE |
publisher.none.fl_str_mv |
PLoS ONE |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/14658/1/artigo-inpa.pdf https://repositorio.inpa.gov.br/bitstream/1/14658/2/license_rdf |
bitstream.checksum.fl_str_mv |
fb56bab5e5dd5ee5f9df2a494390a313 4d2950bda3d176f570a9f8b328dfbbef |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
|
_version_ |
1809928862935547905 |