Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/14508 |
Resumo: | BACKGROUND Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about Zika immunopathogenesis. OBJECTIVES To define the immunologic biomarkers that correlate with acute ZIKV infection. METHODS We characterized the levels of circulating cytokines, chemokines, and growth factors in 54 infected patients of both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison to 100 age-matched controls was performed for statistical analysis and data mining. FINDINGS ZIKV-infected patients present a striking systemic inflammatory response with high levels of pro-inflammatory mediators. Despite the strong inflammatory pattern, IL-1Ra and IL-4 are also induced during the acute infection. Interestingly, the inflammatory cytokines IL-1β, IL-13, IL-17, TNF-α, and IFN-γ; chemokines CXCL8, CCL2, CCL5; and the growth factor G-CSF, displayed a bimodal distribution accompanying viremia. While this is the first manuscript to document bimodal distributions of viremia in ZIKV infection, this has been documented in other viral infections, with a primary viremia peak during mild systemic disease and a secondary peak associated with distribution of the virus to organs and tissues. MAIN CONCLUSIONS Biomarker network analysis demonstrated distinct dynamics in concurrence with the bimodal viremia profiles at different time points during ZIKV infection. Such a robust cytokine and chemokine response has been associated with blood-brain barrier permeability and neuroinvasiveness in other flaviviral infections. High-dimensional data analysis further identified CXCL10, a chemokine involved in foetal neuron apoptosis and Guillain-Barré syndrome, as the most promising biomarker of acute ZIKV infection for potential clinical application. © 2018, Fundacao Oswaldo Cruz. All rights reserved. |
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Naveca, Felipe GomesPontes, Gemilson SoaresChang, Aileen Yu HenSilva, George Allan Villarouco daNascimento, Valdinete Alves doMonteiro, Dana Cristina da SilvaSilva, Marineide Souza daAbdalla, Lígia FernandesSantos, João Hugo AbdallaAlmeida, Tatiana Amaral Pires deMejía, Matilde Del Carmen ContrerasMesquita, Tirza Gabrielle Ramos deEncarnação, Helia Valeria de SouzaGomes, Matheus SouzaAmaral, L. RodriguesCampi-Azevedo, Ana CarolinaCoelho-Dos-Reis, Jordana Grazziela AlvesAntonelli, Lis R.Teixeira-Carvalho, AndréaMartins-Filho, Olindo AssisRamasawmy, Rajendranath2020-04-24T16:49:21Z2020-04-24T16:49:21Z2018https://repositorio.inpa.gov.br/handle/1/1450810.1590/0074-02760170542BACKGROUND Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about Zika immunopathogenesis. OBJECTIVES To define the immunologic biomarkers that correlate with acute ZIKV infection. METHODS We characterized the levels of circulating cytokines, chemokines, and growth factors in 54 infected patients of both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison to 100 age-matched controls was performed for statistical analysis and data mining. FINDINGS ZIKV-infected patients present a striking systemic inflammatory response with high levels of pro-inflammatory mediators. Despite the strong inflammatory pattern, IL-1Ra and IL-4 are also induced during the acute infection. Interestingly, the inflammatory cytokines IL-1β, IL-13, IL-17, TNF-α, and IFN-γ; chemokines CXCL8, CCL2, CCL5; and the growth factor G-CSF, displayed a bimodal distribution accompanying viremia. While this is the first manuscript to document bimodal distributions of viremia in ZIKV infection, this has been documented in other viral infections, with a primary viremia peak during mild systemic disease and a secondary peak associated with distribution of the virus to organs and tissues. MAIN CONCLUSIONS Biomarker network analysis demonstrated distinct dynamics in concurrence with the bimodal viremia profiles at different time points during ZIKV infection. Such a robust cytokine and chemokine response has been associated with blood-brain barrier permeability and neuroinvasiveness in other flaviviral infections. High-dimensional data analysis further identified CXCL10, a chemokine involved in foetal neuron apoptosis and Guillain-Barré syndrome, as the most promising biomarker of acute ZIKV infection for potential clinical application. © 2018, Fundacao Oswaldo Cruz. All rights reserved.Volume 113, Número 6Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBiological MarkerChemokineCytokineGamma Interferon Inducible Protein 10Acute DiseaseAdultBloodCase Control StudyComplicationCross-sectional StudyFemaleGene ExpressionHumanImmunologyMaleMiddle AgedZika FeverAcute DiseaseAdultBiomarkersCase-control StudiesChemokine Cxcl10ChemokinesCross-sectional StudiesCytokinesFemaleGene ExpressionHumansMaleMiddle AgedZika Virus InfectionAnalysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damageinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMemórias do Instituto Oswaldo Cruzengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf6355504https://repositorio.inpa.gov.br/bitstream/1/14508/1/artigo-inpa.pdf2097f9a1a6734b199142ba8d8ccbf14bMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14508/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/145082020-07-14 09:03:54.472oai:repositorio:1/14508Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T13:03:54Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
title |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
spellingShingle |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage Naveca, Felipe Gomes Biological Marker Chemokine Cytokine Gamma Interferon Inducible Protein 10 Acute Disease Adult Blood Case Control Study Complication Cross-sectional Study Female Gene Expression Human Immunology Male Middle Aged Zika Fever Acute Disease Adult Biomarkers Case-control Studies Chemokine Cxcl10 Chemokines Cross-sectional Studies Cytokines Female Gene Expression Humans Male Middle Aged Zika Virus Infection |
title_short |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
title_full |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
title_fullStr |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
title_full_unstemmed |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
title_sort |
Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage |
author |
Naveca, Felipe Gomes |
author_facet |
Naveca, Felipe Gomes Pontes, Gemilson Soares Chang, Aileen Yu Hen Silva, George Allan Villarouco da Nascimento, Valdinete Alves do Monteiro, Dana Cristina da Silva Silva, Marineide Souza da Abdalla, Lígia Fernandes Santos, João Hugo Abdalla Almeida, Tatiana Amaral Pires de Mejía, Matilde Del Carmen Contreras Mesquita, Tirza Gabrielle Ramos de Encarnação, Helia Valeria de Souza Gomes, Matheus Souza Amaral, L. Rodrigues Campi-Azevedo, Ana Carolina Coelho-Dos-Reis, Jordana Grazziela Alves Antonelli, Lis R. Teixeira-Carvalho, Andréa Martins-Filho, Olindo Assis Ramasawmy, Rajendranath |
author_role |
author |
author2 |
Pontes, Gemilson Soares Chang, Aileen Yu Hen Silva, George Allan Villarouco da Nascimento, Valdinete Alves do Monteiro, Dana Cristina da Silva Silva, Marineide Souza da Abdalla, Lígia Fernandes Santos, João Hugo Abdalla Almeida, Tatiana Amaral Pires de Mejía, Matilde Del Carmen Contreras Mesquita, Tirza Gabrielle Ramos de Encarnação, Helia Valeria de Souza Gomes, Matheus Souza Amaral, L. Rodrigues Campi-Azevedo, Ana Carolina Coelho-Dos-Reis, Jordana Grazziela Alves Antonelli, Lis R. Teixeira-Carvalho, Andréa Martins-Filho, Olindo Assis Ramasawmy, Rajendranath |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Naveca, Felipe Gomes Pontes, Gemilson Soares Chang, Aileen Yu Hen Silva, George Allan Villarouco da Nascimento, Valdinete Alves do Monteiro, Dana Cristina da Silva Silva, Marineide Souza da Abdalla, Lígia Fernandes Santos, João Hugo Abdalla Almeida, Tatiana Amaral Pires de Mejía, Matilde Del Carmen Contreras Mesquita, Tirza Gabrielle Ramos de Encarnação, Helia Valeria de Souza Gomes, Matheus Souza Amaral, L. Rodrigues Campi-Azevedo, Ana Carolina Coelho-Dos-Reis, Jordana Grazziela Alves Antonelli, Lis R. Teixeira-Carvalho, Andréa Martins-Filho, Olindo Assis Ramasawmy, Rajendranath |
dc.subject.eng.fl_str_mv |
Biological Marker Chemokine Cytokine Gamma Interferon Inducible Protein 10 Acute Disease Adult Blood Case Control Study Complication Cross-sectional Study Female Gene Expression Human Immunology Male Middle Aged Zika Fever Acute Disease Adult Biomarkers Case-control Studies Chemokine Cxcl10 Chemokines Cross-sectional Studies Cytokines Female Gene Expression Humans Male Middle Aged Zika Virus Infection |
topic |
Biological Marker Chemokine Cytokine Gamma Interferon Inducible Protein 10 Acute Disease Adult Blood Case Control Study Complication Cross-sectional Study Female Gene Expression Human Immunology Male Middle Aged Zika Fever Acute Disease Adult Biomarkers Case-control Studies Chemokine Cxcl10 Chemokines Cross-sectional Studies Cytokines Female Gene Expression Humans Male Middle Aged Zika Virus Infection |
description |
BACKGROUND Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about Zika immunopathogenesis. OBJECTIVES To define the immunologic biomarkers that correlate with acute ZIKV infection. METHODS We characterized the levels of circulating cytokines, chemokines, and growth factors in 54 infected patients of both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison to 100 age-matched controls was performed for statistical analysis and data mining. FINDINGS ZIKV-infected patients present a striking systemic inflammatory response with high levels of pro-inflammatory mediators. Despite the strong inflammatory pattern, IL-1Ra and IL-4 are also induced during the acute infection. Interestingly, the inflammatory cytokines IL-1β, IL-13, IL-17, TNF-α, and IFN-γ; chemokines CXCL8, CCL2, CCL5; and the growth factor G-CSF, displayed a bimodal distribution accompanying viremia. While this is the first manuscript to document bimodal distributions of viremia in ZIKV infection, this has been documented in other viral infections, with a primary viremia peak during mild systemic disease and a secondary peak associated with distribution of the virus to organs and tissues. MAIN CONCLUSIONS Biomarker network analysis demonstrated distinct dynamics in concurrence with the bimodal viremia profiles at different time points during ZIKV infection. Such a robust cytokine and chemokine response has been associated with blood-brain barrier permeability and neuroinvasiveness in other flaviviral infections. High-dimensional data analysis further identified CXCL10, a chemokine involved in foetal neuron apoptosis and Guillain-Barré syndrome, as the most promising biomarker of acute ZIKV infection for potential clinical application. © 2018, Fundacao Oswaldo Cruz. All rights reserved. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-04-24T16:49:21Z |
dc.date.available.fl_str_mv |
2020-04-24T16:49:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://repositorio.inpa.gov.br/handle/1/14508 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760170542 |
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https://repositorio.inpa.gov.br/handle/1/14508 |
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10.1590/0074-02760170542 |
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eng |
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eng |
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Volume 113, Número 6 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Memórias do Instituto Oswaldo Cruz |
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Memórias do Instituto Oswaldo Cruz |
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