Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage

Detalhes bibliográficos
Autor(a) principal: Naveca, Felipe Gomes
Data de Publicação: 2018
Outros Autores: Pontes, Gemilson Soares, Chang, Aileen Yu Hen, Silva, George Allan Villarouco da, Nascimento, Valdinete Alves do, Monteiro, Dana Cristina da Silva, Silva, Marineide Souza da, Abdalla, Lígia Fernandes, Santos, João Hugo Abdalla, Almeida, Tatiana Amaral Pires de, Mejía, Matilde Del Carmen Contreras, Mesquita, Tirza Gabrielle Ramos de, Encarnação, Helia Valeria de Souza, Gomes, Matheus Souza, Amaral, L. Rodrigues, Campi-Azevedo, Ana Carolina, Coelho-Dos-Reis, Jordana Grazziela Alves, Antonelli, Lis R., Teixeira-Carvalho, Andréa, Martins-Filho, Olindo Assis, Ramasawmy, Rajendranath
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14508
Resumo: BACKGROUND Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about Zika immunopathogenesis. OBJECTIVES To define the immunologic biomarkers that correlate with acute ZIKV infection. METHODS We characterized the levels of circulating cytokines, chemokines, and growth factors in 54 infected patients of both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison to 100 age-matched controls was performed for statistical analysis and data mining. FINDINGS ZIKV-infected patients present a striking systemic inflammatory response with high levels of pro-inflammatory mediators. Despite the strong inflammatory pattern, IL-1Ra and IL-4 are also induced during the acute infection. Interestingly, the inflammatory cytokines IL-1β, IL-13, IL-17, TNF-α, and IFN-γ; chemokines CXCL8, CCL2, CCL5; and the growth factor G-CSF, displayed a bimodal distribution accompanying viremia. While this is the first manuscript to document bimodal distributions of viremia in ZIKV infection, this has been documented in other viral infections, with a primary viremia peak during mild systemic disease and a secondary peak associated with distribution of the virus to organs and tissues. MAIN CONCLUSIONS Biomarker network analysis demonstrated distinct dynamics in concurrence with the bimodal viremia profiles at different time points during ZIKV infection. Such a robust cytokine and chemokine response has been associated with blood-brain barrier permeability and neuroinvasiveness in other flaviviral infections. High-dimensional data analysis further identified CXCL10, a chemokine involved in foetal neuron apoptosis and Guillain-Barré syndrome, as the most promising biomarker of acute ZIKV infection for potential clinical application. © 2018, Fundacao Oswaldo Cruz. All rights reserved.
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spelling Naveca, Felipe GomesPontes, Gemilson SoaresChang, Aileen Yu HenSilva, George Allan Villarouco daNascimento, Valdinete Alves doMonteiro, Dana Cristina da SilvaSilva, Marineide Souza daAbdalla, Lígia FernandesSantos, João Hugo AbdallaAlmeida, Tatiana Amaral Pires deMejía, Matilde Del Carmen ContrerasMesquita, Tirza Gabrielle Ramos deEncarnação, Helia Valeria de SouzaGomes, Matheus SouzaAmaral, L. RodriguesCampi-Azevedo, Ana CarolinaCoelho-Dos-Reis, Jordana Grazziela AlvesAntonelli, Lis R.Teixeira-Carvalho, AndréaMartins-Filho, Olindo AssisRamasawmy, Rajendranath2020-04-24T16:49:21Z2020-04-24T16:49:21Z2018https://repositorio.inpa.gov.br/handle/1/1450810.1590/0074-02760170542BACKGROUND Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about Zika immunopathogenesis. OBJECTIVES To define the immunologic biomarkers that correlate with acute ZIKV infection. METHODS We characterized the levels of circulating cytokines, chemokines, and growth factors in 54 infected patients of both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison to 100 age-matched controls was performed for statistical analysis and data mining. FINDINGS ZIKV-infected patients present a striking systemic inflammatory response with high levels of pro-inflammatory mediators. Despite the strong inflammatory pattern, IL-1Ra and IL-4 are also induced during the acute infection. Interestingly, the inflammatory cytokines IL-1β, IL-13, IL-17, TNF-α, and IFN-γ; chemokines CXCL8, CCL2, CCL5; and the growth factor G-CSF, displayed a bimodal distribution accompanying viremia. While this is the first manuscript to document bimodal distributions of viremia in ZIKV infection, this has been documented in other viral infections, with a primary viremia peak during mild systemic disease and a secondary peak associated with distribution of the virus to organs and tissues. MAIN CONCLUSIONS Biomarker network analysis demonstrated distinct dynamics in concurrence with the bimodal viremia profiles at different time points during ZIKV infection. Such a robust cytokine and chemokine response has been associated with blood-brain barrier permeability and neuroinvasiveness in other flaviviral infections. High-dimensional data analysis further identified CXCL10, a chemokine involved in foetal neuron apoptosis and Guillain-Barré syndrome, as the most promising biomarker of acute ZIKV infection for potential clinical application. © 2018, Fundacao Oswaldo Cruz. All rights reserved.Volume 113, Número 6Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBiological MarkerChemokineCytokineGamma Interferon Inducible Protein 10Acute DiseaseAdultBloodCase Control StudyComplicationCross-sectional StudyFemaleGene ExpressionHumanImmunologyMaleMiddle AgedZika FeverAcute DiseaseAdultBiomarkersCase-control StudiesChemokine Cxcl10ChemokinesCross-sectional StudiesCytokinesFemaleGene ExpressionHumansMaleMiddle AgedZika Virus InfectionAnalysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damageinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMemórias do Instituto Oswaldo Cruzengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf6355504https://repositorio.inpa.gov.br/bitstream/1/14508/1/artigo-inpa.pdf2097f9a1a6734b199142ba8d8ccbf14bMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14508/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/145082020-07-14 09:03:54.472oai:repositorio:1/14508Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T13:03:54Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
title Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
spellingShingle Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
Naveca, Felipe Gomes
Biological Marker
Chemokine
Cytokine
Gamma Interferon Inducible Protein 10
Acute Disease
Adult
Blood
Case Control Study
Complication
Cross-sectional Study
Female
Gene Expression
Human
Immunology
Male
Middle Aged
Zika Fever
Acute Disease
Adult
Biomarkers
Case-control Studies
Chemokine Cxcl10
Chemokines
Cross-sectional Studies
Cytokines
Female
Gene Expression
Humans
Male
Middle Aged
Zika Virus Infection
title_short Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
title_full Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
title_fullStr Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
title_full_unstemmed Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
title_sort Analysis of the immunological biomarker profile during acute zika virus infection reveals the overexpression of CXCL10, a chemokine linked to neuronal damage
author Naveca, Felipe Gomes
author_facet Naveca, Felipe Gomes
Pontes, Gemilson Soares
Chang, Aileen Yu Hen
Silva, George Allan Villarouco da
Nascimento, Valdinete Alves do
Monteiro, Dana Cristina da Silva
Silva, Marineide Souza da
Abdalla, Lígia Fernandes
Santos, João Hugo Abdalla
Almeida, Tatiana Amaral Pires de
Mejía, Matilde Del Carmen Contreras
Mesquita, Tirza Gabrielle Ramos de
Encarnação, Helia Valeria de Souza
Gomes, Matheus Souza
Amaral, L. Rodrigues
Campi-Azevedo, Ana Carolina
Coelho-Dos-Reis, Jordana Grazziela Alves
Antonelli, Lis R.
Teixeira-Carvalho, Andréa
Martins-Filho, Olindo Assis
Ramasawmy, Rajendranath
author_role author
author2 Pontes, Gemilson Soares
Chang, Aileen Yu Hen
Silva, George Allan Villarouco da
Nascimento, Valdinete Alves do
Monteiro, Dana Cristina da Silva
Silva, Marineide Souza da
Abdalla, Lígia Fernandes
Santos, João Hugo Abdalla
Almeida, Tatiana Amaral Pires de
Mejía, Matilde Del Carmen Contreras
Mesquita, Tirza Gabrielle Ramos de
Encarnação, Helia Valeria de Souza
Gomes, Matheus Souza
Amaral, L. Rodrigues
Campi-Azevedo, Ana Carolina
Coelho-Dos-Reis, Jordana Grazziela Alves
Antonelli, Lis R.
Teixeira-Carvalho, Andréa
Martins-Filho, Olindo Assis
Ramasawmy, Rajendranath
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Naveca, Felipe Gomes
Pontes, Gemilson Soares
Chang, Aileen Yu Hen
Silva, George Allan Villarouco da
Nascimento, Valdinete Alves do
Monteiro, Dana Cristina da Silva
Silva, Marineide Souza da
Abdalla, Lígia Fernandes
Santos, João Hugo Abdalla
Almeida, Tatiana Amaral Pires de
Mejía, Matilde Del Carmen Contreras
Mesquita, Tirza Gabrielle Ramos de
Encarnação, Helia Valeria de Souza
Gomes, Matheus Souza
Amaral, L. Rodrigues
Campi-Azevedo, Ana Carolina
Coelho-Dos-Reis, Jordana Grazziela Alves
Antonelli, Lis R.
Teixeira-Carvalho, Andréa
Martins-Filho, Olindo Assis
Ramasawmy, Rajendranath
dc.subject.eng.fl_str_mv Biological Marker
Chemokine
Cytokine
Gamma Interferon Inducible Protein 10
Acute Disease
Adult
Blood
Case Control Study
Complication
Cross-sectional Study
Female
Gene Expression
Human
Immunology
Male
Middle Aged
Zika Fever
Acute Disease
Adult
Biomarkers
Case-control Studies
Chemokine Cxcl10
Chemokines
Cross-sectional Studies
Cytokines
Female
Gene Expression
Humans
Male
Middle Aged
Zika Virus Infection
topic Biological Marker
Chemokine
Cytokine
Gamma Interferon Inducible Protein 10
Acute Disease
Adult
Blood
Case Control Study
Complication
Cross-sectional Study
Female
Gene Expression
Human
Immunology
Male
Middle Aged
Zika Fever
Acute Disease
Adult
Biomarkers
Case-control Studies
Chemokine Cxcl10
Chemokines
Cross-sectional Studies
Cytokines
Female
Gene Expression
Humans
Male
Middle Aged
Zika Virus Infection
description BACKGROUND Infection with Zika virus (ZIKV) manifests in a broad spectrum of disease ranging from mild illness to severe neurological complications and little is known about Zika immunopathogenesis. OBJECTIVES To define the immunologic biomarkers that correlate with acute ZIKV infection. METHODS We characterized the levels of circulating cytokines, chemokines, and growth factors in 54 infected patients of both genders at five different time points after symptom onset using microbeads multiplex immunoassay; comparison to 100 age-matched controls was performed for statistical analysis and data mining. FINDINGS ZIKV-infected patients present a striking systemic inflammatory response with high levels of pro-inflammatory mediators. Despite the strong inflammatory pattern, IL-1Ra and IL-4 are also induced during the acute infection. Interestingly, the inflammatory cytokines IL-1β, IL-13, IL-17, TNF-α, and IFN-γ; chemokines CXCL8, CCL2, CCL5; and the growth factor G-CSF, displayed a bimodal distribution accompanying viremia. While this is the first manuscript to document bimodal distributions of viremia in ZIKV infection, this has been documented in other viral infections, with a primary viremia peak during mild systemic disease and a secondary peak associated with distribution of the virus to organs and tissues. MAIN CONCLUSIONS Biomarker network analysis demonstrated distinct dynamics in concurrence with the bimodal viremia profiles at different time points during ZIKV infection. Such a robust cytokine and chemokine response has been associated with blood-brain barrier permeability and neuroinvasiveness in other flaviviral infections. High-dimensional data analysis further identified CXCL10, a chemokine involved in foetal neuron apoptosis and Guillain-Barré syndrome, as the most promising biomarker of acute ZIKV infection for potential clinical application. © 2018, Fundacao Oswaldo Cruz. All rights reserved.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2020-04-24T16:49:21Z
dc.date.available.fl_str_mv 2020-04-24T16:49:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14508
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760170542
url https://repositorio.inpa.gov.br/handle/1/14508
identifier_str_mv 10.1590/0074-02760170542
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 113, Número 6
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
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