Partial characterization of amylases of two indigenous central amazonian rhizobia strains
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16270 |
Resumo: | Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-midexponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 °C. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain. |
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Oliveira, Arlem Nascimento deOliveira, Luiz Antonio deAndrade, Jerusa Souza2020-06-02T20:08:34Z2020-06-02T20:08:34Z2010https://repositorio.inpa.gov.br/handle/1/1627010.1590/S1516-89132010000100005Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-midexponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 °C. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain.Volume 53, Número 1, Pags. 35-45Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBradyrhizobiumRhizobiaceaeRhizobiumPartial characterization of amylases of two indigenous central amazonian rhizobia strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Archives of Biology and Technologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf143551https://repositorio.inpa.gov.br/bitstream/1/16270/1/artigo-inpa.pdf27993137f29ff4b4ccd90ceef977ef1dMD511/162702020-06-02 16:43:37.557oai:repositorio:1/16270Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-02T20:43:37Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
title |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
spellingShingle |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains Oliveira, Arlem Nascimento de Bradyrhizobium Rhizobiaceae Rhizobium |
title_short |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
title_full |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
title_fullStr |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
title_full_unstemmed |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
title_sort |
Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
author |
Oliveira, Arlem Nascimento de |
author_facet |
Oliveira, Arlem Nascimento de Oliveira, Luiz Antonio de Andrade, Jerusa Souza |
author_role |
author |
author2 |
Oliveira, Luiz Antonio de Andrade, Jerusa Souza |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Oliveira, Arlem Nascimento de Oliveira, Luiz Antonio de Andrade, Jerusa Souza |
dc.subject.eng.fl_str_mv |
Bradyrhizobium Rhizobiaceae Rhizobium |
topic |
Bradyrhizobium Rhizobiaceae Rhizobium |
description |
Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-midexponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 °C. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2020-06-02T20:08:34Z |
dc.date.available.fl_str_mv |
2020-06-02T20:08:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16270 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1516-89132010000100005 |
url |
https://repositorio.inpa.gov.br/handle/1/16270 |
identifier_str_mv |
10.1590/S1516-89132010000100005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 53, Número 1, Pags. 35-45 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Brazilian Archives of Biology and Technology |
publisher.none.fl_str_mv |
Brazilian Archives of Biology and Technology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/16270/1/artigo-inpa.pdf |
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27993137f29ff4b4ccd90ceef977ef1d |
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MD5 |
repository.name.fl_str_mv |
Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
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1801499124666728448 |