Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains

Detalhes bibliográficos
Autor(a) principal: Oliveira,Arlem Nascimento de
Data de Publicação: 2010
Outros Autores: Oliveira,Luiz Antonio de, Andrade,Jerusa Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024
Resumo: Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).
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spelling Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strainsRhizobiumBradyrhizobiumenzymatic activitiesorganic surfactantsTwo rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).Instituto de Tecnologia do Paraná - Tecpar2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024Brazilian Archives of Biology and Technology v.53 n.5 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000500024info:eu-repo/semantics/openAccessOliveira,Arlem Nascimento deOliveira,Luiz Antonio deAndrade,Jerusa Souzaeng2010-11-03T00:00:00Zoai:scielo:S1516-89132010000500024Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-11-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
title Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
spellingShingle Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
Oliveira,Arlem Nascimento de
Rhizobium
Bradyrhizobium
enzymatic activities
organic surfactants
title_short Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
title_full Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
title_fullStr Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
title_full_unstemmed Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
title_sort Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
author Oliveira,Arlem Nascimento de
author_facet Oliveira,Arlem Nascimento de
Oliveira,Luiz Antonio de
Andrade,Jerusa Souza
author_role author
author2 Oliveira,Luiz Antonio de
Andrade,Jerusa Souza
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira,Arlem Nascimento de
Oliveira,Luiz Antonio de
Andrade,Jerusa Souza
dc.subject.por.fl_str_mv Rhizobium
Bradyrhizobium
enzymatic activities
organic surfactants
topic Rhizobium
Bradyrhizobium
enzymatic activities
organic surfactants
description Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000500024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.5 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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