Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation

Detalhes bibliográficos
Autor(a) principal: Steinmacher, Douglas André
Data de Publicação: 2007
Outros Autores: Krohn, N. G., Dantas, A. C.M., Stefenon, Valdir Marcos, Clement, Charles Roland, Guerra, Miguel Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16346
Resumo: • Background and Aims: The thin cell layer (TCL) technique is based on the use of very small explants and has allowed enhanced in vitro morphogenesis in several plant species. The present study evaluated the TCL technique as a procedure for somatic embryo production and plantlet regeneration of peach palm. • Methods: TCL explants from different positions in the shoot apex and leaf sheath of peach palm were cultivated in MS culture medium supplemented with 0-600 μm Picloram in the presence of activated charcoal. The production of primary calli and embryogenic calli was evaluated in these different conditions. Histological and amplified fragment length polymorphism (AFLP) analyses were conducted to study in vitro morphogenetic responses and genetic stability, respectively, of the regenerated plantlets. • Key Results: Abundant primary callus induction was observed from TCLs of the shoot meristem in culture media supplemented with 150-600 μm Picloram (83-97 %, respectively). The production of embryogenic calli depends on Picloram concentration and explant position. The best response observed was 43 % embryogenic callus production from shoot meristem TCL on 300 μm Picloram. In maturation conditions, 34 ± 4 somatic embryos per embryogenic callus were obtained, and 45.0 ± 3.4 % of these fully developed somatic embryos were converted, resulting in plantlets ready for acclimatization, of which 80 % survived. Histological studies revealed that the first cellular division events occurred in cells adjacent to vascular tissue, resulting in primary calli, whose growth was ensured by a meristematic zone. A multicellular origin of the resulting somatic embryos arising from the meristematic zone is suggested. During maturation, histological analyses revealed bipolarization of the somatic embryos, as well as the development of new somatic embryos. AFLP analyses revealed that 92 % of the regenerated plantlets were true to type. The use of TCL explants considerably improves the number of calli and somatic embryos produced in comparison with previously described protocols for in vitro regeneration of peach palm. • Conclusions: The present study suggests that the TCL somatic embryogenesis protocol developed is feasible, although it still requires further optimization for in vitro multiplication of peach palm, especially the use of similar explants obtained from adult palm trees. © The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.
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spelling Steinmacher, Douglas AndréKrohn, N. G.Dantas, A. C.M.Stefenon, Valdir MarcosClement, Charles RolandGuerra, Miguel Pedro2020-06-03T21:27:32Z2020-06-03T21:27:32Z2007https://repositorio.inpa.gov.br/handle/1/1634610.1093/aob/mcm153• Background and Aims: The thin cell layer (TCL) technique is based on the use of very small explants and has allowed enhanced in vitro morphogenesis in several plant species. The present study evaluated the TCL technique as a procedure for somatic embryo production and plantlet regeneration of peach palm. • Methods: TCL explants from different positions in the shoot apex and leaf sheath of peach palm were cultivated in MS culture medium supplemented with 0-600 μm Picloram in the presence of activated charcoal. The production of primary calli and embryogenic calli was evaluated in these different conditions. Histological and amplified fragment length polymorphism (AFLP) analyses were conducted to study in vitro morphogenetic responses and genetic stability, respectively, of the regenerated plantlets. • Key Results: Abundant primary callus induction was observed from TCLs of the shoot meristem in culture media supplemented with 150-600 μm Picloram (83-97 %, respectively). The production of embryogenic calli depends on Picloram concentration and explant position. The best response observed was 43 % embryogenic callus production from shoot meristem TCL on 300 μm Picloram. In maturation conditions, 34 ± 4 somatic embryos per embryogenic callus were obtained, and 45.0 ± 3.4 % of these fully developed somatic embryos were converted, resulting in plantlets ready for acclimatization, of which 80 % survived. Histological studies revealed that the first cellular division events occurred in cells adjacent to vascular tissue, resulting in primary calli, whose growth was ensured by a meristematic zone. A multicellular origin of the resulting somatic embryos arising from the meristematic zone is suggested. During maturation, histological analyses revealed bipolarization of the somatic embryos, as well as the development of new somatic embryos. AFLP analyses revealed that 92 % of the regenerated plantlets were true to type. The use of TCL explants considerably improves the number of calli and somatic embryos produced in comparison with previously described protocols for in vitro regeneration of peach palm. • Conclusions: The present study suggests that the TCL somatic embryogenesis protocol developed is feasible, although it still requires further optimization for in vitro multiplication of peach palm, especially the use of similar explants obtained from adult palm trees. © The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.Volume 100, Número 4, Pags. 699-709Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessPicloramHerbicideMolecular AnalysisMonocotyledonAmplified Fragment Length PolymorphismArecaceaeCloningCytologyDrug EffectEmbryo DevelopmentEvaluationGeneticsMeristemMethodologyPhysiologyPlantPrenatal DevelopmentAmplified Fragment Length Polymorphism AnalysisArecaceaeCloning, OrganismEmbryonic DevelopmentMeristemPicloramPlant ShootsBactris GasipaesPrunus PersicaBactris GasipaesSomatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnnals of Botanyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf1080136https://repositorio.inpa.gov.br/bitstream/1/16346/1/artigo-inpa.pdfac44142abef604fe58104ae8abec40d7MD511/163462020-06-03 18:14:24.905oai:repositorio:1/16346Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-03T22:14:24Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
title Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
spellingShingle Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
Steinmacher, Douglas André
Picloram
Herbicide
Molecular Analysis
Monocotyledon
Amplified Fragment Length Polymorphism
Arecaceae
Cloning
Cytology
Drug Effect
Embryo Development
Evaluation
Genetics
Meristem
Methodology
Physiology
Plant
Prenatal Development
Amplified Fragment Length Polymorphism Analysis
Arecaceae
Cloning, Organism
Embryonic Development
Meristem
Picloram
Plant Shoots
Bactris Gasipaes
Prunus Persica
Bactris Gasipaes
title_short Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
title_full Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
title_fullStr Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
title_full_unstemmed Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
title_sort Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morpho-histological aspects and AFLP analysis of somaclonal variation
author Steinmacher, Douglas André
author_facet Steinmacher, Douglas André
Krohn, N. G.
Dantas, A. C.M.
Stefenon, Valdir Marcos
Clement, Charles Roland
Guerra, Miguel Pedro
author_role author
author2 Krohn, N. G.
Dantas, A. C.M.
Stefenon, Valdir Marcos
Clement, Charles Roland
Guerra, Miguel Pedro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Steinmacher, Douglas André
Krohn, N. G.
Dantas, A. C.M.
Stefenon, Valdir Marcos
Clement, Charles Roland
Guerra, Miguel Pedro
dc.subject.eng.fl_str_mv Picloram
Herbicide
Molecular Analysis
Monocotyledon
Amplified Fragment Length Polymorphism
Arecaceae
Cloning
Cytology
Drug Effect
Embryo Development
Evaluation
Genetics
Meristem
Methodology
Physiology
Plant
Prenatal Development
Amplified Fragment Length Polymorphism Analysis
Arecaceae
Cloning, Organism
Embryonic Development
Meristem
Picloram
Plant Shoots
Bactris Gasipaes
Prunus Persica
Bactris Gasipaes
topic Picloram
Herbicide
Molecular Analysis
Monocotyledon
Amplified Fragment Length Polymorphism
Arecaceae
Cloning
Cytology
Drug Effect
Embryo Development
Evaluation
Genetics
Meristem
Methodology
Physiology
Plant
Prenatal Development
Amplified Fragment Length Polymorphism Analysis
Arecaceae
Cloning, Organism
Embryonic Development
Meristem
Picloram
Plant Shoots
Bactris Gasipaes
Prunus Persica
Bactris Gasipaes
description • Background and Aims: The thin cell layer (TCL) technique is based on the use of very small explants and has allowed enhanced in vitro morphogenesis in several plant species. The present study evaluated the TCL technique as a procedure for somatic embryo production and plantlet regeneration of peach palm. • Methods: TCL explants from different positions in the shoot apex and leaf sheath of peach palm were cultivated in MS culture medium supplemented with 0-600 μm Picloram in the presence of activated charcoal. The production of primary calli and embryogenic calli was evaluated in these different conditions. Histological and amplified fragment length polymorphism (AFLP) analyses were conducted to study in vitro morphogenetic responses and genetic stability, respectively, of the regenerated plantlets. • Key Results: Abundant primary callus induction was observed from TCLs of the shoot meristem in culture media supplemented with 150-600 μm Picloram (83-97 %, respectively). The production of embryogenic calli depends on Picloram concentration and explant position. The best response observed was 43 % embryogenic callus production from shoot meristem TCL on 300 μm Picloram. In maturation conditions, 34 ± 4 somatic embryos per embryogenic callus were obtained, and 45.0 ± 3.4 % of these fully developed somatic embryos were converted, resulting in plantlets ready for acclimatization, of which 80 % survived. Histological studies revealed that the first cellular division events occurred in cells adjacent to vascular tissue, resulting in primary calli, whose growth was ensured by a meristematic zone. A multicellular origin of the resulting somatic embryos arising from the meristematic zone is suggested. During maturation, histological analyses revealed bipolarization of the somatic embryos, as well as the development of new somatic embryos. AFLP analyses revealed that 92 % of the regenerated plantlets were true to type. The use of TCL explants considerably improves the number of calli and somatic embryos produced in comparison with previously described protocols for in vitro regeneration of peach palm. • Conclusions: The present study suggests that the TCL somatic embryogenesis protocol developed is feasible, although it still requires further optimization for in vitro multiplication of peach palm, especially the use of similar explants obtained from adult palm trees. © The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2020-06-03T21:27:32Z
dc.date.available.fl_str_mv 2020-06-03T21:27:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16346
dc.identifier.doi.none.fl_str_mv 10.1093/aob/mcm153
url https://repositorio.inpa.gov.br/handle/1/16346
identifier_str_mv 10.1093/aob/mcm153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 100, Número 4, Pags. 699-709
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Annals of Botany
publisher.none.fl_str_mv Annals of Botany
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reponame_str Repositório Institucional do INPA
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