Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization

Detalhes bibliográficos
Autor(a) principal: Silva, H. C.M.
Data de Publicação: 2017
Outros Autores: Martins-Júnior, M. M.C., Braga Ribeiro, Leila, Matoso, D. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/15017
Resumo: The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors.
id INPA-2_841075bb0bb13d83ee05c946ccb5e57c
oai_identifier_str oai:repositorio:1/15017
network_acronym_str INPA-2
network_name_str Repositório Institucional do INPA
repository_id_str
spelling Silva, H. C.M.Martins-Júnior, M. M.C.Braga Ribeiro, LeilaMatoso, D. A.2020-05-07T14:00:36Z2020-05-07T14:00:36Z2017https://repositorio.inpa.gov.br/handle/1/1501710.4238/gmr16019658The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors.Volume 16, Número 1Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBiotinDigoxigeninAlgorithmChromosomeColorControlled StudyIn Situ Hybridization, FluorescenceImage AnalysisQuantitative AnalysisEvaluation StudyIn Situ Hybridization, FluorescenceMicroscopy, FluorescenceImage ProcessingNucleic Acid HybridizationProceduresSoftwareChromosomesEvaluation Studies As TopicImage Processing, Computer-assistedIn Situ Hybridization, FluorescenceMicroscopy, FluorescenceNucleic Acid HybridizationSoftwareFluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf302084https://repositorio.inpa.gov.br/bitstream/1/15017/1/artigo-inpa.pdf71a57aecc4902f66ba7a7196e3879e74MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15017/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/150172020-07-14 10:30:53.451oai:repositorio:1/15017Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:53Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
title Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
spellingShingle Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
Silva, H. C.M.
Biotin
Digoxigenin
Algorithm
Chromosome
Color
Controlled Study
In Situ Hybridization, Fluorescence
Image Analysis
Quantitative Analysis
Evaluation Study
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Image Processing
Nucleic Acid Hybridization
Procedures
Software
Chromosomes
Evaluation Studies As Topic
Image Processing, Computer-assisted
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Nucleic Acid Hybridization
Software
title_short Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
title_full Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
title_fullStr Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
title_full_unstemmed Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
title_sort Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
author Silva, H. C.M.
author_facet Silva, H. C.M.
Martins-Júnior, M. M.C.
Braga Ribeiro, Leila
Matoso, D. A.
author_role author
author2 Martins-Júnior, M. M.C.
Braga Ribeiro, Leila
Matoso, D. A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, H. C.M.
Martins-Júnior, M. M.C.
Braga Ribeiro, Leila
Matoso, D. A.
dc.subject.eng.fl_str_mv Biotin
Digoxigenin
Algorithm
Chromosome
Color
Controlled Study
In Situ Hybridization, Fluorescence
Image Analysis
Quantitative Analysis
Evaluation Study
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Image Processing
Nucleic Acid Hybridization
Procedures
Software
Chromosomes
Evaluation Studies As Topic
Image Processing, Computer-assisted
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Nucleic Acid Hybridization
Software
topic Biotin
Digoxigenin
Algorithm
Chromosome
Color
Controlled Study
In Situ Hybridization, Fluorescence
Image Analysis
Quantitative Analysis
Evaluation Study
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Image Processing
Nucleic Acid Hybridization
Procedures
Software
Chromosomes
Evaluation Studies As Topic
Image Processing, Computer-assisted
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Nucleic Acid Hybridization
Software
description The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2020-05-07T14:00:36Z
dc.date.available.fl_str_mv 2020-05-07T14:00:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/15017
dc.identifier.doi.none.fl_str_mv 10.4238/gmr16019658
url https://repositorio.inpa.gov.br/handle/1/15017
identifier_str_mv 10.4238/gmr16019658
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 16, Número 1
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics and Molecular Research
publisher.none.fl_str_mv Genetics and Molecular Research
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/15017/1/artigo-inpa.pdf
https://repositorio.inpa.gov.br/bitstream/1/15017/2/license_rdf
bitstream.checksum.fl_str_mv 71a57aecc4902f66ba7a7196e3879e74
4d2950bda3d176f570a9f8b328dfbbef
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv
_version_ 1809928868993171456

Registros relacionados