Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fabiana da Rocha
Data de Publicação: 2017
Outros Autores: Noronha, Maria das Dores Nogueira, Lozano, Jorge Luis López
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/15170
Resumo: Introduction: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. Methods: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. Results and Conclusions: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.
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spelling Oliveira, Fabiana da RochaNoronha, Maria das Dores NogueiraLozano, Jorge Luis López2020-05-07T14:08:31Z2020-05-07T14:08:31Z2017https://repositorio.inpa.gov.br/handle/1/1517010.1590/0037-8682-0408-2016Introduction: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. Methods: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. Results and Conclusions: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.Volume 50, Número 3, Pags. 365-373Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessHyaluronidasePhospholipase A2Snake VenomSnake Venom AntiserumPhospholipase A2Animals ExperimentAnimals ModelAnticoagulationBiological ActivityBleedingEnzyme ActivityHypersensitivityLd 50Micrurus SurinamensisMouseNeurotoxicityNonhumanElectrophoresis, Polyacrylamide GelProtein DegradationSnakeWestern BlottingZymographyAnimalsBagg Albino MouseChemistryCross ReactionElapidaeElectrophoresisEnzymologyGeneticsMetabolismAnimalCross ReactionsElapid VenomsElapidaeElectrophoresisLethal Dose 50MiceMice, Inbred Balb CPhospholipases A2Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista da Sociedade Brasileira de Medicina Tropicalengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf3421806https://repositorio.inpa.gov.br/bitstream/1/15170/1/artigo-inpa.pdf27e50f40c509f0496b82a773dc57bdf7MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15170/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/151702020-07-14 10:55:40.16oai:repositorio:1/15170Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:55:40Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
spellingShingle Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
Oliveira, Fabiana da Rocha
Hyaluronidase
Phospholipase A2
Snake Venom
Snake Venom Antiserum
Phospholipase A2
Animals Experiment
Animals Model
Anticoagulation
Biological Activity
Bleeding
Enzyme Activity
Hypersensitivity
Ld 50
Micrurus Surinamensis
Mouse
Neurotoxicity
Nonhuman
Electrophoresis, Polyacrylamide Gel
Protein Degradation
Snake
Western Blotting
Zymography
Animals
Bagg Albino Mouse
Chemistry
Cross Reaction
Elapidae
Electrophoresis
Enzymology
Genetics
Metabolism
Animal
Cross Reactions
Elapid Venoms
Elapidae
Electrophoresis
Lethal Dose 50
Mice
Mice, Inbred Balb C
Phospholipases A2
title_short Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_full Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_fullStr Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_full_unstemmed Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_sort Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
author Oliveira, Fabiana da Rocha
author_facet Oliveira, Fabiana da Rocha
Noronha, Maria das Dores Nogueira
Lozano, Jorge Luis López
author_role author
author2 Noronha, Maria das Dores Nogueira
Lozano, Jorge Luis López
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira, Fabiana da Rocha
Noronha, Maria das Dores Nogueira
Lozano, Jorge Luis López
dc.subject.eng.fl_str_mv Hyaluronidase
Phospholipase A2
Snake Venom
Snake Venom Antiserum
Phospholipase A2
Animals Experiment
Animals Model
Anticoagulation
Biological Activity
Bleeding
Enzyme Activity
Hypersensitivity
Ld 50
Micrurus Surinamensis
Mouse
Neurotoxicity
Nonhuman
Electrophoresis, Polyacrylamide Gel
Protein Degradation
Snake
Western Blotting
Zymography
Animals
Bagg Albino Mouse
Chemistry
Cross Reaction
Elapidae
Electrophoresis
Enzymology
Genetics
Metabolism
Animal
Cross Reactions
Elapid Venoms
Elapidae
Electrophoresis
Lethal Dose 50
Mice
Mice, Inbred Balb C
Phospholipases A2
topic Hyaluronidase
Phospholipase A2
Snake Venom
Snake Venom Antiserum
Phospholipase A2
Animals Experiment
Animals Model
Anticoagulation
Biological Activity
Bleeding
Enzyme Activity
Hypersensitivity
Ld 50
Micrurus Surinamensis
Mouse
Neurotoxicity
Nonhuman
Electrophoresis, Polyacrylamide Gel
Protein Degradation
Snake
Western Blotting
Zymography
Animals
Bagg Albino Mouse
Chemistry
Cross Reaction
Elapidae
Electrophoresis
Enzymology
Genetics
Metabolism
Animal
Cross Reactions
Elapid Venoms
Elapidae
Electrophoresis
Lethal Dose 50
Mice
Mice, Inbred Balb C
Phospholipases A2
description Introduction: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. Methods: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. Results and Conclusions: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2020-05-07T14:08:31Z
dc.date.available.fl_str_mv 2020-05-07T14:08:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/15170
dc.identifier.doi.none.fl_str_mv 10.1590/0037-8682-0408-2016
url https://repositorio.inpa.gov.br/handle/1/15170
identifier_str_mv 10.1590/0037-8682-0408-2016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 50, Número 3, Pags. 365-373
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/15170/1/artigo-inpa.pdf
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