Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15170 |
Resumo: | Introduction: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. Methods: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. Results and Conclusions: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved. |
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Oliveira, Fabiana da RochaNoronha, Maria das Dores NogueiraLozano, Jorge Luis López2020-05-07T14:08:31Z2020-05-07T14:08:31Z2017https://repositorio.inpa.gov.br/handle/1/1517010.1590/0037-8682-0408-2016Introduction: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. Methods: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. Results and Conclusions: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.Volume 50, Número 3, Pags. 365-373Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessHyaluronidasePhospholipase A2Snake VenomSnake Venom AntiserumPhospholipase A2Animals ExperimentAnimals ModelAnticoagulationBiological ActivityBleedingEnzyme ActivityHypersensitivityLd 50Micrurus SurinamensisMouseNeurotoxicityNonhumanElectrophoresis, Polyacrylamide GelProtein DegradationSnakeWestern BlottingZymographyAnimalsBagg Albino MouseChemistryCross ReactionElapidaeElectrophoresisEnzymologyGeneticsMetabolismAnimalCross ReactionsElapid VenomsElapidaeElectrophoresisLethal Dose 50MiceMice, Inbred Balb CPhospholipases A2Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista da Sociedade Brasileira de Medicina Tropicalengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf3421806https://repositorio.inpa.gov.br/bitstream/1/15170/1/artigo-inpa.pdf27e50f40c509f0496b82a773dc57bdf7MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15170/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/151702020-07-14 10:55:40.16oai:repositorio:1/15170Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:55:40Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
title |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
spellingShingle |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis Oliveira, Fabiana da Rocha Hyaluronidase Phospholipase A2 Snake Venom Snake Venom Antiserum Phospholipase A2 Animals Experiment Animals Model Anticoagulation Biological Activity Bleeding Enzyme Activity Hypersensitivity Ld 50 Micrurus Surinamensis Mouse Neurotoxicity Nonhuman Electrophoresis, Polyacrylamide Gel Protein Degradation Snake Western Blotting Zymography Animals Bagg Albino Mouse Chemistry Cross Reaction Elapidae Electrophoresis Enzymology Genetics Metabolism Animal Cross Reactions Elapid Venoms Elapidae Electrophoresis Lethal Dose 50 Mice Mice, Inbred Balb C Phospholipases A2 |
title_short |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
title_full |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
title_fullStr |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
title_full_unstemmed |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
title_sort |
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis |
author |
Oliveira, Fabiana da Rocha |
author_facet |
Oliveira, Fabiana da Rocha Noronha, Maria das Dores Nogueira Lozano, Jorge Luis López |
author_role |
author |
author2 |
Noronha, Maria das Dores Nogueira Lozano, Jorge Luis López |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Oliveira, Fabiana da Rocha Noronha, Maria das Dores Nogueira Lozano, Jorge Luis López |
dc.subject.eng.fl_str_mv |
Hyaluronidase Phospholipase A2 Snake Venom Snake Venom Antiserum Phospholipase A2 Animals Experiment Animals Model Anticoagulation Biological Activity Bleeding Enzyme Activity Hypersensitivity Ld 50 Micrurus Surinamensis Mouse Neurotoxicity Nonhuman Electrophoresis, Polyacrylamide Gel Protein Degradation Snake Western Blotting Zymography Animals Bagg Albino Mouse Chemistry Cross Reaction Elapidae Electrophoresis Enzymology Genetics Metabolism Animal Cross Reactions Elapid Venoms Elapidae Electrophoresis Lethal Dose 50 Mice Mice, Inbred Balb C Phospholipases A2 |
topic |
Hyaluronidase Phospholipase A2 Snake Venom Snake Venom Antiserum Phospholipase A2 Animals Experiment Animals Model Anticoagulation Biological Activity Bleeding Enzyme Activity Hypersensitivity Ld 50 Micrurus Surinamensis Mouse Neurotoxicity Nonhuman Electrophoresis, Polyacrylamide Gel Protein Degradation Snake Western Blotting Zymography Animals Bagg Albino Mouse Chemistry Cross Reaction Elapidae Electrophoresis Enzymology Genetics Metabolism Animal Cross Reactions Elapid Venoms Elapidae Electrophoresis Lethal Dose 50 Mice Mice, Inbred Balb C Phospholipases A2 |
description |
Introduction: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. Methods: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. Results and Conclusions: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-05-07T14:08:31Z |
dc.date.available.fl_str_mv |
2020-05-07T14:08:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15170 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0037-8682-0408-2016 |
url |
https://repositorio.inpa.gov.br/handle/1/15170 |
identifier_str_mv |
10.1590/0037-8682-0408-2016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 50, Número 3, Pags. 365-373 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical |
publisher.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical |
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Repositório Institucional do INPA |
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