A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/23257 |
Resumo: | This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods. ©2020 Sociedade Brasileira de Química |
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Mor, Natalia CristinaCorreia, Banny S.B.Val, Adalberto LuisTasic, L.2020-07-03T21:06:33Z2020-07-03T21:06:33Z2020https://repositorio.inpa.gov.br/handle/1/2325710.21577/0103-5053.20190230This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods. ©2020 Sociedade Brasileira de QuímicaVolume 31, Número 4, Pags. 662-672Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAmazonian fishLipid analysisNuclear Magnetic Resonance SpectroscopyA protocol for fish lipid analysis using nuclear magnetic resonance spectroscopyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of the Brazilian Chemical Societyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALprotocol.pdfprotocol.pdfapplication/pdf4363045https://repositorio.inpa.gov.br/bitstream/1/23257/1/protocol.pdfd2d9a5ed4bb03531cf3439b4638771aaMD511/232572020-07-16 12:37:58.022oai:repositorio:1/23257Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-16T16:37:58Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
title |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
spellingShingle |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy Mor, Natalia Cristina Amazonian fish Lipid analysis Nuclear Magnetic Resonance Spectroscopy |
title_short |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
title_full |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
title_fullStr |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
title_full_unstemmed |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
title_sort |
A protocol for fish lipid analysis using nuclear magnetic resonance spectroscopy |
author |
Mor, Natalia Cristina |
author_facet |
Mor, Natalia Cristina Correia, Banny S.B. Val, Adalberto Luis Tasic, L. |
author_role |
author |
author2 |
Correia, Banny S.B. Val, Adalberto Luis Tasic, L. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mor, Natalia Cristina Correia, Banny S.B. Val, Adalberto Luis Tasic, L. |
dc.subject.eng.fl_str_mv |
Amazonian fish Lipid analysis Nuclear Magnetic Resonance Spectroscopy |
topic |
Amazonian fish Lipid analysis Nuclear Magnetic Resonance Spectroscopy |
description |
This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods. ©2020 Sociedade Brasileira de Química |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-07-03T21:06:33Z |
dc.date.available.fl_str_mv |
2020-07-03T21:06:33Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/23257 |
dc.identifier.doi.none.fl_str_mv |
10.21577/0103-5053.20190230 |
url |
https://repositorio.inpa.gov.br/handle/1/23257 |
identifier_str_mv |
10.21577/0103-5053.20190230 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 31, Número 4, Pags. 662-672 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Journal of the Brazilian Chemical Society |
publisher.none.fl_str_mv |
Journal of the Brazilian Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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Instituto Nacional de Pesquisas da Amazônia (INPA) |
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INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
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Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/23257/1/protocol.pdf |
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Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
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1809928885895168000 |