Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species

Detalhes bibliográficos
Autor(a) principal: Cruz-Hernández, Pedro
Data de Publicação: 2013
Outros Autores: Batista, Jacqueline da Silva, Formiga, Kyara M., Rafael, Míriam Silva, Tadei, Wanderli Pedro, Santos, Joselita Maria M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
DOI: 10.4238/2013.March.13.11
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14993
Resumo: Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP.
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spelling Cruz-Hernández, PedroBatista, Jacqueline da SilvaFormiga, Kyara M.Rafael, Míriam SilvaTadei, Wanderli PedroSantos, Joselita Maria M.2020-05-07T14:00:23Z2020-05-07T14:00:23Z2013https://repositorio.inpa.gov.br/handle/1/1499310.4238/2013.March.13.11Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP.Volume 12, Número 3, Pags. 3088-3092Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessGenomic DnaAlleleAnophelesAnopheles TriannulatusBrasilDna HybridizationGene AmplificationGene LocusGenetic LinkageHeterozygosityMicrosatellite MarkerNonhumanNucleotide SequencePolymorphic Dna Microsatellite LocusGenetics, PopulationAllelesAnimalAnophelesBrasilDnaGenetics, PopulationHumansLinkage DisequilibriumMicrosatellite RepeatsPolymorphism, GeneticSpecies SpecificityIsolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf303985https://repositorio.inpa.gov.br/bitstream/1/14993/1/artigo-inpa.pdfa57c29d828bc3e30a0fde65e49691739MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14993/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149932020-07-14 10:30:37.956oai:repositorio:1/14993Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:37Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
title Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
spellingShingle Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
Cruz-Hernández, Pedro
Genomic Dna
Allele
Anopheles
Anopheles Triannulatus
Brasil
Dna Hybridization
Gene Amplification
Gene Locus
Genetic Linkage
Heterozygosity
Microsatellite Marker
Nonhuman
Nucleotide Sequence
Polymorphic Dna Microsatellite Locus
Genetics, Population
Alleles
Animal
Anopheles
Brasil
Dna
Genetics, Population
Humans
Linkage Disequilibrium
Microsatellite Repeats
Polymorphism, Genetic
Species Specificity
title_short Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
title_full Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
title_fullStr Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
title_full_unstemmed Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
title_sort Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
author Cruz-Hernández, Pedro
author_facet Cruz-Hernández, Pedro
Batista, Jacqueline da Silva
Formiga, Kyara M.
Rafael, Míriam Silva
Tadei, Wanderli Pedro
Santos, Joselita Maria M.
author_role author
author2 Batista, Jacqueline da Silva
Formiga, Kyara M.
Rafael, Míriam Silva
Tadei, Wanderli Pedro
Santos, Joselita Maria M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz-Hernández, Pedro
Batista, Jacqueline da Silva
Formiga, Kyara M.
Rafael, Míriam Silva
Tadei, Wanderli Pedro
Santos, Joselita Maria M.
dc.subject.eng.fl_str_mv Genomic Dna
Allele
Anopheles
Anopheles Triannulatus
Brasil
Dna Hybridization
Gene Amplification
Gene Locus
Genetic Linkage
Heterozygosity
Microsatellite Marker
Nonhuman
Nucleotide Sequence
Polymorphic Dna Microsatellite Locus
Genetics, Population
Alleles
Animal
Anopheles
Brasil
Dna
Genetics, Population
Humans
Linkage Disequilibrium
Microsatellite Repeats
Polymorphism, Genetic
Species Specificity
topic Genomic Dna
Allele
Anopheles
Anopheles Triannulatus
Brasil
Dna Hybridization
Gene Amplification
Gene Locus
Genetic Linkage
Heterozygosity
Microsatellite Marker
Nonhuman
Nucleotide Sequence
Polymorphic Dna Microsatellite Locus
Genetics, Population
Alleles
Animal
Anopheles
Brasil
Dna
Genetics, Population
Humans
Linkage Disequilibrium
Microsatellite Repeats
Polymorphism, Genetic
Species Specificity
description Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2020-05-07T14:00:23Z
dc.date.available.fl_str_mv 2020-05-07T14:00:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14993
dc.identifier.doi.none.fl_str_mv 10.4238/2013.March.13.11
url https://repositorio.inpa.gov.br/handle/1/14993
identifier_str_mv 10.4238/2013.March.13.11
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 12, Número 3, Pags. 3088-3092
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics and Molecular Research
publisher.none.fl_str_mv Genetics and Molecular Research
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
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reponame_str Repositório Institucional do INPA
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