Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
DOI: | 10.4238/2013.March.13.11 |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/14993 |
Resumo: | Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP. |
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Cruz-Hernández, PedroBatista, Jacqueline da SilvaFormiga, Kyara M.Rafael, Míriam SilvaTadei, Wanderli PedroSantos, Joselita Maria M.2020-05-07T14:00:23Z2020-05-07T14:00:23Z2013https://repositorio.inpa.gov.br/handle/1/1499310.4238/2013.March.13.11Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP.Volume 12, Número 3, Pags. 3088-3092Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessGenomic DnaAlleleAnophelesAnopheles TriannulatusBrasilDna HybridizationGene AmplificationGene LocusGenetic LinkageHeterozygosityMicrosatellite MarkerNonhumanNucleotide SequencePolymorphic Dna Microsatellite LocusGenetics, PopulationAllelesAnimalAnophelesBrasilDnaGenetics, PopulationHumansLinkage DisequilibriumMicrosatellite RepeatsPolymorphism, GeneticSpecies SpecificityIsolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf303985https://repositorio.inpa.gov.br/bitstream/1/14993/1/artigo-inpa.pdfa57c29d828bc3e30a0fde65e49691739MD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14993/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149932020-07-14 10:30:37.956oai:repositorio:1/14993Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:37Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
title |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
spellingShingle |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species Cruz-Hernández, Pedro Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity |
title_short |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
title_full |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
title_fullStr |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
title_full_unstemmed |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
title_sort |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
author |
Cruz-Hernández, Pedro |
author_facet |
Cruz-Hernández, Pedro Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. |
author_role |
author |
author2 |
Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cruz-Hernández, Pedro Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. |
dc.subject.eng.fl_str_mv |
Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity |
topic |
Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity |
description |
Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2020-05-07T14:00:23Z |
dc.date.available.fl_str_mv |
2020-05-07T14:00:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/14993 |
dc.identifier.doi.none.fl_str_mv |
10.4238/2013.March.13.11 |
url |
https://repositorio.inpa.gov.br/handle/1/14993 |
identifier_str_mv |
10.4238/2013.March.13.11 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 12, Número 3, Pags. 3088-3092 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Genetics and Molecular Research |
publisher.none.fl_str_mv |
Genetics and Molecular Research |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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INPA |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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