Seeds of Amazonian Fabaceae as a source of new lectins
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16157 |
Resumo: | Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera Anadenanthera, Dimorphandra and Ormosia. The study of lectins from these genera and from Swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures. |
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Fernandes, Andréia VarmesRamos, Márcio VianaGonçalves, José Francisco Carvalho deMaranhão, Paulo Abraão C.Chevreuil, Larissa RamosSouza, Luis Augusto Gomes2020-05-25T19:10:48Z2020-05-25T19:10:48Z2011https://repositorio.inpa.gov.br/handle/1/1615710.1590/S1677-04202011000300008Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera Anadenanthera, Dimorphandra and Ormosia. The study of lectins from these genera and from Swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures.Volume 23, Número 3, Pags. 237-244Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAnadenantheraAnadenanthera PeregrinaCaesalpinioideaeCaudataDimorphandraFabaceaeMimosoideaeOrmosia (angiosperm)Ormosia LignivalvisPapilionoideaeSwartziaSeeds of Amazonian Fabaceae as a source of new lectinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Plant Physiologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf352217https://repositorio.inpa.gov.br/bitstream/1/16157/1/artigo-inpa.pdf88901d7089c5023e31de0459c99f5f57MD511/161572020-05-25 16:32:57.283oai:repositorio:1/16157Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-25T20:32:57Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Seeds of Amazonian Fabaceae as a source of new lectins |
title |
Seeds of Amazonian Fabaceae as a source of new lectins |
spellingShingle |
Seeds of Amazonian Fabaceae as a source of new lectins Fernandes, Andréia Varmes Anadenanthera Anadenanthera Peregrina Caesalpinioideae Caudata Dimorphandra Fabaceae Mimosoideae Ormosia (angiosperm) Ormosia Lignivalvis Papilionoideae Swartzia |
title_short |
Seeds of Amazonian Fabaceae as a source of new lectins |
title_full |
Seeds of Amazonian Fabaceae as a source of new lectins |
title_fullStr |
Seeds of Amazonian Fabaceae as a source of new lectins |
title_full_unstemmed |
Seeds of Amazonian Fabaceae as a source of new lectins |
title_sort |
Seeds of Amazonian Fabaceae as a source of new lectins |
author |
Fernandes, Andréia Varmes |
author_facet |
Fernandes, Andréia Varmes Ramos, Márcio Viana Gonçalves, José Francisco Carvalho de Maranhão, Paulo Abraão C. Chevreuil, Larissa Ramos Souza, Luis Augusto Gomes |
author_role |
author |
author2 |
Ramos, Márcio Viana Gonçalves, José Francisco Carvalho de Maranhão, Paulo Abraão C. Chevreuil, Larissa Ramos Souza, Luis Augusto Gomes |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Andréia Varmes Ramos, Márcio Viana Gonçalves, José Francisco Carvalho de Maranhão, Paulo Abraão C. Chevreuil, Larissa Ramos Souza, Luis Augusto Gomes |
dc.subject.eng.fl_str_mv |
Anadenanthera Anadenanthera Peregrina Caesalpinioideae Caudata Dimorphandra Fabaceae Mimosoideae Ormosia (angiosperm) Ormosia Lignivalvis Papilionoideae Swartzia |
topic |
Anadenanthera Anadenanthera Peregrina Caesalpinioideae Caudata Dimorphandra Fabaceae Mimosoideae Ormosia (angiosperm) Ormosia Lignivalvis Papilionoideae Swartzia |
description |
Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera Anadenanthera, Dimorphandra and Ormosia. The study of lectins from these genera and from Swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2020-05-25T19:10:48Z |
dc.date.available.fl_str_mv |
2020-05-25T19:10:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16157 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1677-04202011000300008 |
url |
https://repositorio.inpa.gov.br/handle/1/16157 |
identifier_str_mv |
10.1590/S1677-04202011000300008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 23, Número 3, Pags. 237-244 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Brazilian Journal of Plant Physiology |
publisher.none.fl_str_mv |
Brazilian Journal of Plant Physiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Repositório Institucional do INPA |
collection |
Repositório Institucional do INPA |
bitstream.url.fl_str_mv |
https://repositorio.inpa.gov.br/bitstream/1/16157/1/artigo-inpa.pdf |
bitstream.checksum.fl_str_mv |
88901d7089c5023e31de0459c99f5f57 |
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repository.name.fl_str_mv |
Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA) |
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