Seeds of Amazonian Fabaceae as a source of new lectins

Detalhes bibliográficos
Autor(a) principal: Fernandes, Andréia Varmes
Data de Publicação: 2011
Outros Autores: Ramos, Márcio Viana, Gonçalves, José Francisco Carvalho de, Maranhão, Paulo Abraão C., Chevreuil, Larissa Ramos, Souza, Luis Augusto Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16157
Resumo: Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera Anadenanthera, Dimorphandra and Ormosia. The study of lectins from these genera and from Swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures.
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spelling Fernandes, Andréia VarmesRamos, Márcio VianaGonçalves, José Francisco Carvalho deMaranhão, Paulo Abraão C.Chevreuil, Larissa RamosSouza, Luis Augusto Gomes2020-05-25T19:10:48Z2020-05-25T19:10:48Z2011https://repositorio.inpa.gov.br/handle/1/1615710.1590/S1677-04202011000300008Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera Anadenanthera, Dimorphandra and Ormosia. The study of lectins from these genera and from Swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures.Volume 23, Número 3, Pags. 237-244Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAnadenantheraAnadenanthera PeregrinaCaesalpinioideaeCaudataDimorphandraFabaceaeMimosoideaeOrmosia (angiosperm)Ormosia LignivalvisPapilionoideaeSwartziaSeeds of Amazonian Fabaceae as a source of new lectinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Plant Physiologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf352217https://repositorio.inpa.gov.br/bitstream/1/16157/1/artigo-inpa.pdf88901d7089c5023e31de0459c99f5f57MD511/161572020-05-25 16:32:57.283oai:repositorio:1/16157Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-25T20:32:57Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Seeds of Amazonian Fabaceae as a source of new lectins
title Seeds of Amazonian Fabaceae as a source of new lectins
spellingShingle Seeds of Amazonian Fabaceae as a source of new lectins
Fernandes, Andréia Varmes
Anadenanthera
Anadenanthera Peregrina
Caesalpinioideae
Caudata
Dimorphandra
Fabaceae
Mimosoideae
Ormosia (angiosperm)
Ormosia Lignivalvis
Papilionoideae
Swartzia
title_short Seeds of Amazonian Fabaceae as a source of new lectins
title_full Seeds of Amazonian Fabaceae as a source of new lectins
title_fullStr Seeds of Amazonian Fabaceae as a source of new lectins
title_full_unstemmed Seeds of Amazonian Fabaceae as a source of new lectins
title_sort Seeds of Amazonian Fabaceae as a source of new lectins
author Fernandes, Andréia Varmes
author_facet Fernandes, Andréia Varmes
Ramos, Márcio Viana
Gonçalves, José Francisco Carvalho de
Maranhão, Paulo Abraão C.
Chevreuil, Larissa Ramos
Souza, Luis Augusto Gomes
author_role author
author2 Ramos, Márcio Viana
Gonçalves, José Francisco Carvalho de
Maranhão, Paulo Abraão C.
Chevreuil, Larissa Ramos
Souza, Luis Augusto Gomes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Andréia Varmes
Ramos, Márcio Viana
Gonçalves, José Francisco Carvalho de
Maranhão, Paulo Abraão C.
Chevreuil, Larissa Ramos
Souza, Luis Augusto Gomes
dc.subject.eng.fl_str_mv Anadenanthera
Anadenanthera Peregrina
Caesalpinioideae
Caudata
Dimorphandra
Fabaceae
Mimosoideae
Ormosia (angiosperm)
Ormosia Lignivalvis
Papilionoideae
Swartzia
topic Anadenanthera
Anadenanthera Peregrina
Caesalpinioideae
Caudata
Dimorphandra
Fabaceae
Mimosoideae
Ormosia (angiosperm)
Ormosia Lignivalvis
Papilionoideae
Swartzia
description Seeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by galactose or lactose but not by glucose or mannose. The crude extract of S. laevicarpa exhibited HA activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. Despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera Anadenanthera, Dimorphandra and Ormosia. The study of lectins from these genera and from Swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2020-05-25T19:10:48Z
dc.date.available.fl_str_mv 2020-05-25T19:10:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16157
dc.identifier.doi.none.fl_str_mv 10.1590/S1677-04202011000300008
url https://repositorio.inpa.gov.br/handle/1/16157
identifier_str_mv 10.1590/S1677-04202011000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 23, Número 3, Pags. 237-244
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Brazilian Journal of Plant Physiology
publisher.none.fl_str_mv Brazilian Journal of Plant Physiology
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
bitstream.url.fl_str_mv https://repositorio.inpa.gov.br/bitstream/1/16157/1/artigo-inpa.pdf
bitstream.checksum.fl_str_mv 88901d7089c5023e31de0459c99f5f57
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repository.name.fl_str_mv Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)
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