Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)

Detalhes bibliográficos
Autor(a) principal: Siqueira, Sérgio
Data de Publicação: 2009
Outros Autores: Aguiar-Júnior, Odair, Souza, Moisés Barbosa, Lima, Albertina Pimental, Pimentel, Shirlei Maria Recco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
DOI: 10.1111/j.1601-5223.2009.02104.x
Texto Completo: https://repositorio.inpa.gov.br/handle/1/16299
Resumo: We report a cytogenetic analysis of three Pristimantis fenestratus frog populations of the Amazon region in Brazil. The specimens were sampled in Borba and Manaus, Amazonas State, and in Rio Branco, Acre State. They were analyzed using Giemsa, silver staining, C-banding and FISH with rDNA probes. The karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of Ag-NORs and in the heterochromatin pattern as well. The NOR was located on the pairs 05 and 07 in the Rio Branco specimens, pair 10 in the Manaus specimens, and pair 1 in the Borba specimens. A small C-band was detected on the telomeric region of the pair 05 in the Borba population, while in the Manaus there was a heterochromatic block adjacent to the centromere of pair 9. An unusual intra-individual variation of chromosome number was observed in metaphases of Rio Branco specimens, comprising fundamental numbers of 33, 34 and 35. Additionally, interchromosome thread connections were detected between telomere - telomere, centromere - telomere and centromere - centromere regions, and among chromosomal heterochromatin-rich sites. The NOR sites were also involved in those connections. We hypothesize that this variation is due to chromosome missegregation during mitosis. The inter- and intraindividual variation in chromosome number suggests chromosomal instability in P. fenestratus, which has not been detected in any other anuran group. Since Borba is the type-locality of P. fenestratus, a taxonomic review of the Manaus and Rio Branco populations should be done, as indicated by the cytogenetic evidence that they could be new species of Pristimantis. © 2009 The Authors.
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spelling Siqueira, SérgioAguiar-Júnior, OdairSouza, Moisés BarbosaLima, Albertina PimentalPimentel, Shirlei Maria Recco2020-06-03T01:55:49Z2020-06-03T01:55:49Z2009https://repositorio.inpa.gov.br/handle/1/1629910.1111/j.1601-5223.2009.02104.xWe report a cytogenetic analysis of three Pristimantis fenestratus frog populations of the Amazon region in Brazil. The specimens were sampled in Borba and Manaus, Amazonas State, and in Rio Branco, Acre State. They were analyzed using Giemsa, silver staining, C-banding and FISH with rDNA probes. The karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of Ag-NORs and in the heterochromatin pattern as well. The NOR was located on the pairs 05 and 07 in the Rio Branco specimens, pair 10 in the Manaus specimens, and pair 1 in the Borba specimens. A small C-band was detected on the telomeric region of the pair 05 in the Borba population, while in the Manaus there was a heterochromatic block adjacent to the centromere of pair 9. An unusual intra-individual variation of chromosome number was observed in metaphases of Rio Branco specimens, comprising fundamental numbers of 33, 34 and 35. Additionally, interchromosome thread connections were detected between telomere - telomere, centromere - telomere and centromere - centromere regions, and among chromosomal heterochromatin-rich sites. The NOR sites were also involved in those connections. We hypothesize that this variation is due to chromosome missegregation during mitosis. The inter- and intraindividual variation in chromosome number suggests chromosomal instability in P. fenestratus, which has not been detected in any other anuran group. Since Borba is the type-locality of P. fenestratus, a taxonomic review of the Manaus and Rio Branco populations should be done, as indicated by the cytogenetic evidence that they could be new species of Pristimantis. © 2009 The Authors.Volume 146, Número 4, Pags. 141-151Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessRibosome DnaAnimals TissueBrasilC BandingCentromereChromosomal InstabilityChromosome AnalysisChromosome NumberChromosome SegregationControlled StudyCytogeneticsDna ProbeFemaleIn Situ Hybridization, FluorescenceFrogGiemsa StainHeterochromatinHypothesisKaryotypeMaleMetaphaseMitosisNew SpeciesNonhumanSilver StainingTaxonomyTelomereAnimalAnuraBrasilChromosomesCytogenetic AnalysisGenetic VariationGenetics, PopulationIn Situ Hybridization, FluorescenceKaryotypingSpecies SpecificityAnuraProtomelas FenestratusUnusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHereditasengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf3731642https://repositorio.inpa.gov.br/bitstream/1/16299/1/artigo-inpa.pdfc087e84cfcb19d5b932f191cf6695157MD511/162992020-06-02 22:48:30.593oai:repositorio:1/16299Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-03T02:48:30Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
title Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
spellingShingle Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
Siqueira, Sérgio
Ribosome Dna
Animals Tissue
Brasil
C Banding
Centromere
Chromosomal Instability
Chromosome Analysis
Chromosome Number
Chromosome Segregation
Controlled Study
Cytogenetics
Dna Probe
Female
In Situ Hybridization, Fluorescence
Frog
Giemsa Stain
Heterochromatin
Hypothesis
Karyotype
Male
Metaphase
Mitosis
New Species
Nonhuman
Silver Staining
Taxonomy
Telomere
Animal
Anura
Brasil
Chromosomes
Cytogenetic Analysis
Genetic Variation
Genetics, Population
In Situ Hybridization, Fluorescence
Karyotyping
Species Specificity
Anura
Protomelas Fenestratus
title_short Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
title_full Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
title_fullStr Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
title_full_unstemmed Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
title_sort Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
author Siqueira, Sérgio
author_facet Siqueira, Sérgio
Aguiar-Júnior, Odair
Souza, Moisés Barbosa
Lima, Albertina Pimental
Pimentel, Shirlei Maria Recco
author_role author
author2 Aguiar-Júnior, Odair
Souza, Moisés Barbosa
Lima, Albertina Pimental
Pimentel, Shirlei Maria Recco
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Siqueira, Sérgio
Aguiar-Júnior, Odair
Souza, Moisés Barbosa
Lima, Albertina Pimental
Pimentel, Shirlei Maria Recco
dc.subject.eng.fl_str_mv Ribosome Dna
Animals Tissue
Brasil
C Banding
Centromere
Chromosomal Instability
Chromosome Analysis
Chromosome Number
Chromosome Segregation
Controlled Study
Cytogenetics
Dna Probe
Female
In Situ Hybridization, Fluorescence
Frog
Giemsa Stain
Heterochromatin
Hypothesis
Karyotype
Male
Metaphase
Mitosis
New Species
Nonhuman
Silver Staining
Taxonomy
Telomere
Animal
Anura
Brasil
Chromosomes
Cytogenetic Analysis
Genetic Variation
Genetics, Population
In Situ Hybridization, Fluorescence
Karyotyping
Species Specificity
Anura
Protomelas Fenestratus
topic Ribosome Dna
Animals Tissue
Brasil
C Banding
Centromere
Chromosomal Instability
Chromosome Analysis
Chromosome Number
Chromosome Segregation
Controlled Study
Cytogenetics
Dna Probe
Female
In Situ Hybridization, Fluorescence
Frog
Giemsa Stain
Heterochromatin
Hypothesis
Karyotype
Male
Metaphase
Mitosis
New Species
Nonhuman
Silver Staining
Taxonomy
Telomere
Animal
Anura
Brasil
Chromosomes
Cytogenetic Analysis
Genetic Variation
Genetics, Population
In Situ Hybridization, Fluorescence
Karyotyping
Species Specificity
Anura
Protomelas Fenestratus
description We report a cytogenetic analysis of three Pristimantis fenestratus frog populations of the Amazon region in Brazil. The specimens were sampled in Borba and Manaus, Amazonas State, and in Rio Branco, Acre State. They were analyzed using Giemsa, silver staining, C-banding and FISH with rDNA probes. The karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of Ag-NORs and in the heterochromatin pattern as well. The NOR was located on the pairs 05 and 07 in the Rio Branco specimens, pair 10 in the Manaus specimens, and pair 1 in the Borba specimens. A small C-band was detected on the telomeric region of the pair 05 in the Borba population, while in the Manaus there was a heterochromatic block adjacent to the centromere of pair 9. An unusual intra-individual variation of chromosome number was observed in metaphases of Rio Branco specimens, comprising fundamental numbers of 33, 34 and 35. Additionally, interchromosome thread connections were detected between telomere - telomere, centromere - telomere and centromere - centromere regions, and among chromosomal heterochromatin-rich sites. The NOR sites were also involved in those connections. We hypothesize that this variation is due to chromosome missegregation during mitosis. The inter- and intraindividual variation in chromosome number suggests chromosomal instability in P. fenestratus, which has not been detected in any other anuran group. Since Borba is the type-locality of P. fenestratus, a taxonomic review of the Manaus and Rio Branco populations should be done, as indicated by the cytogenetic evidence that they could be new species of Pristimantis. © 2009 The Authors.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2020-06-03T01:55:49Z
dc.date.available.fl_str_mv 2020-06-03T01:55:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/16299
dc.identifier.doi.none.fl_str_mv 10.1111/j.1601-5223.2009.02104.x
url https://repositorio.inpa.gov.br/handle/1/16299
identifier_str_mv 10.1111/j.1601-5223.2009.02104.x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 146, Número 4, Pags. 141-151
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hereditas
publisher.none.fl_str_mv Hereditas
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
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