Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
DOI: | 10.1111/j.1601-5223.2009.02104.x |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16299 |
Resumo: | We report a cytogenetic analysis of three Pristimantis fenestratus frog populations of the Amazon region in Brazil. The specimens were sampled in Borba and Manaus, Amazonas State, and in Rio Branco, Acre State. They were analyzed using Giemsa, silver staining, C-banding and FISH with rDNA probes. The karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of Ag-NORs and in the heterochromatin pattern as well. The NOR was located on the pairs 05 and 07 in the Rio Branco specimens, pair 10 in the Manaus specimens, and pair 1 in the Borba specimens. A small C-band was detected on the telomeric region of the pair 05 in the Borba population, while in the Manaus there was a heterochromatic block adjacent to the centromere of pair 9. An unusual intra-individual variation of chromosome number was observed in metaphases of Rio Branco specimens, comprising fundamental numbers of 33, 34 and 35. Additionally, interchromosome thread connections were detected between telomere - telomere, centromere - telomere and centromere - centromere regions, and among chromosomal heterochromatin-rich sites. The NOR sites were also involved in those connections. We hypothesize that this variation is due to chromosome missegregation during mitosis. The inter- and intraindividual variation in chromosome number suggests chromosomal instability in P. fenestratus, which has not been detected in any other anuran group. Since Borba is the type-locality of P. fenestratus, a taxonomic review of the Manaus and Rio Branco populations should be done, as indicated by the cytogenetic evidence that they could be new species of Pristimantis. © 2009 The Authors. |
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Siqueira, SérgioAguiar-Júnior, OdairSouza, Moisés BarbosaLima, Albertina PimentalPimentel, Shirlei Maria Recco2020-06-03T01:55:49Z2020-06-03T01:55:49Z2009https://repositorio.inpa.gov.br/handle/1/1629910.1111/j.1601-5223.2009.02104.xWe report a cytogenetic analysis of three Pristimantis fenestratus frog populations of the Amazon region in Brazil. The specimens were sampled in Borba and Manaus, Amazonas State, and in Rio Branco, Acre State. They were analyzed using Giemsa, silver staining, C-banding and FISH with rDNA probes. The karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of Ag-NORs and in the heterochromatin pattern as well. The NOR was located on the pairs 05 and 07 in the Rio Branco specimens, pair 10 in the Manaus specimens, and pair 1 in the Borba specimens. A small C-band was detected on the telomeric region of the pair 05 in the Borba population, while in the Manaus there was a heterochromatic block adjacent to the centromere of pair 9. An unusual intra-individual variation of chromosome number was observed in metaphases of Rio Branco specimens, comprising fundamental numbers of 33, 34 and 35. Additionally, interchromosome thread connections were detected between telomere - telomere, centromere - telomere and centromere - centromere regions, and among chromosomal heterochromatin-rich sites. The NOR sites were also involved in those connections. We hypothesize that this variation is due to chromosome missegregation during mitosis. The inter- and intraindividual variation in chromosome number suggests chromosomal instability in P. fenestratus, which has not been detected in any other anuran group. Since Borba is the type-locality of P. fenestratus, a taxonomic review of the Manaus and Rio Branco populations should be done, as indicated by the cytogenetic evidence that they could be new species of Pristimantis. © 2009 The Authors.Volume 146, Número 4, Pags. 141-151Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessRibosome DnaAnimals TissueBrasilC BandingCentromereChromosomal InstabilityChromosome AnalysisChromosome NumberChromosome SegregationControlled StudyCytogeneticsDna ProbeFemaleIn Situ Hybridization, FluorescenceFrogGiemsa StainHeterochromatinHypothesisKaryotypeMaleMetaphaseMitosisNew SpeciesNonhumanSilver StainingTaxonomyTelomereAnimalAnuraBrasilChromosomesCytogenetic AnalysisGenetic VariationGenetics, PopulationIn Situ Hybridization, FluorescenceKaryotypingSpecies SpecificityAnuraProtomelas FenestratusUnusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHereditasengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf3731642https://repositorio.inpa.gov.br/bitstream/1/16299/1/artigo-inpa.pdfc087e84cfcb19d5b932f191cf6695157MD511/162992020-06-02 22:48:30.593oai:repositorio:1/16299Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-06-03T02:48:30Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
title |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
spellingShingle |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) Siqueira, Sérgio Ribosome Dna Animals Tissue Brasil C Banding Centromere Chromosomal Instability Chromosome Analysis Chromosome Number Chromosome Segregation Controlled Study Cytogenetics Dna Probe Female In Situ Hybridization, Fluorescence Frog Giemsa Stain Heterochromatin Hypothesis Karyotype Male Metaphase Mitosis New Species Nonhuman Silver Staining Taxonomy Telomere Animal Anura Brasil Chromosomes Cytogenetic Analysis Genetic Variation Genetics, Population In Situ Hybridization, Fluorescence Karyotyping Species Specificity Anura Protomelas Fenestratus |
title_short |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
title_full |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
title_fullStr |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
title_full_unstemmed |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
title_sort |
Unusual intra-individual karyotypical variation and evidence of cryptic species in Amazonian populations of Pristimantis (Anura, Terrarana) |
author |
Siqueira, Sérgio |
author_facet |
Siqueira, Sérgio Aguiar-Júnior, Odair Souza, Moisés Barbosa Lima, Albertina Pimental Pimentel, Shirlei Maria Recco |
author_role |
author |
author2 |
Aguiar-Júnior, Odair Souza, Moisés Barbosa Lima, Albertina Pimental Pimentel, Shirlei Maria Recco |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Siqueira, Sérgio Aguiar-Júnior, Odair Souza, Moisés Barbosa Lima, Albertina Pimental Pimentel, Shirlei Maria Recco |
dc.subject.eng.fl_str_mv |
Ribosome Dna Animals Tissue Brasil C Banding Centromere Chromosomal Instability Chromosome Analysis Chromosome Number Chromosome Segregation Controlled Study Cytogenetics Dna Probe Female In Situ Hybridization, Fluorescence Frog Giemsa Stain Heterochromatin Hypothesis Karyotype Male Metaphase Mitosis New Species Nonhuman Silver Staining Taxonomy Telomere Animal Anura Brasil Chromosomes Cytogenetic Analysis Genetic Variation Genetics, Population In Situ Hybridization, Fluorescence Karyotyping Species Specificity Anura Protomelas Fenestratus |
topic |
Ribosome Dna Animals Tissue Brasil C Banding Centromere Chromosomal Instability Chromosome Analysis Chromosome Number Chromosome Segregation Controlled Study Cytogenetics Dna Probe Female In Situ Hybridization, Fluorescence Frog Giemsa Stain Heterochromatin Hypothesis Karyotype Male Metaphase Mitosis New Species Nonhuman Silver Staining Taxonomy Telomere Animal Anura Brasil Chromosomes Cytogenetic Analysis Genetic Variation Genetics, Population In Situ Hybridization, Fluorescence Karyotyping Species Specificity Anura Protomelas Fenestratus |
description |
We report a cytogenetic analysis of three Pristimantis fenestratus frog populations of the Amazon region in Brazil. The specimens were sampled in Borba and Manaus, Amazonas State, and in Rio Branco, Acre State. They were analyzed using Giemsa, silver staining, C-banding and FISH with rDNA probes. The karyotypes of the three populations revealed 2n = 34 chromosomes, but they differed in the number and position of Ag-NORs and in the heterochromatin pattern as well. The NOR was located on the pairs 05 and 07 in the Rio Branco specimens, pair 10 in the Manaus specimens, and pair 1 in the Borba specimens. A small C-band was detected on the telomeric region of the pair 05 in the Borba population, while in the Manaus there was a heterochromatic block adjacent to the centromere of pair 9. An unusual intra-individual variation of chromosome number was observed in metaphases of Rio Branco specimens, comprising fundamental numbers of 33, 34 and 35. Additionally, interchromosome thread connections were detected between telomere - telomere, centromere - telomere and centromere - centromere regions, and among chromosomal heterochromatin-rich sites. The NOR sites were also involved in those connections. We hypothesize that this variation is due to chromosome missegregation during mitosis. The inter- and intraindividual variation in chromosome number suggests chromosomal instability in P. fenestratus, which has not been detected in any other anuran group. Since Borba is the type-locality of P. fenestratus, a taxonomic review of the Manaus and Rio Branco populations should be done, as indicated by the cytogenetic evidence that they could be new species of Pristimantis. © 2009 The Authors. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2020-06-03T01:55:49Z |
dc.date.available.fl_str_mv |
2020-06-03T01:55:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16299 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1601-5223.2009.02104.x |
url |
https://repositorio.inpa.gov.br/handle/1/16299 |
identifier_str_mv |
10.1111/j.1601-5223.2009.02104.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 146, Número 4, Pags. 141-151 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Hereditas |
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Hereditas |
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