LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Amazonica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019 |
Resumo: | In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL. |
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LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicusAeromonas hydrophila96-h LD50matrinxãtropical fishesIn order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL.Instituto Nacional de Pesquisas da Amazônia2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019Acta Amazonica v.41 n.2 2011reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/S0044-59672011000200019info:eu-repo/semantics/openAccessOliveira,Sarah Ragonha deSouza,Rondon Tatsuta Yamane Baptista deBrasil,Elenice MartinsAndrade,Jaqueline Inês Alves deNunes,Érica da Silva SantiagoOno,Eduardo AkifumiAffonso,Elizabeth Gusmãoeng2011-05-09T00:00:00Zoai:scielo:S0044-59672011000200019Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2011-05-09T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.none.fl_str_mv |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
title |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
spellingShingle |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus Oliveira,Sarah Ragonha de Aeromonas hydrophila 96-h LD50 matrinxã tropical fishes |
title_short |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
title_full |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
title_fullStr |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
title_full_unstemmed |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
title_sort |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus |
author |
Oliveira,Sarah Ragonha de |
author_facet |
Oliveira,Sarah Ragonha de Souza,Rondon Tatsuta Yamane Baptista de Brasil,Elenice Martins Andrade,Jaqueline Inês Alves de Nunes,Érica da Silva Santiago Ono,Eduardo Akifumi Affonso,Elizabeth Gusmão |
author_role |
author |
author2 |
Souza,Rondon Tatsuta Yamane Baptista de Brasil,Elenice Martins Andrade,Jaqueline Inês Alves de Nunes,Érica da Silva Santiago Ono,Eduardo Akifumi Affonso,Elizabeth Gusmão |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Sarah Ragonha de Souza,Rondon Tatsuta Yamane Baptista de Brasil,Elenice Martins Andrade,Jaqueline Inês Alves de Nunes,Érica da Silva Santiago Ono,Eduardo Akifumi Affonso,Elizabeth Gusmão |
dc.subject.por.fl_str_mv |
Aeromonas hydrophila 96-h LD50 matrinxã tropical fishes |
topic |
Aeromonas hydrophila 96-h LD50 matrinxã tropical fishes |
description |
In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0044-59672011000200019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
Acta Amazonica v.41 n.2 2011 reponame:Acta Amazonica instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Acta Amazonica |
collection |
Acta Amazonica |
repository.name.fl_str_mv |
Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
acta@inpa.gov.br||acta@inpa.gov.br |
_version_ |
1752129839081455616 |